Transcript: Human NM_001278580.1

Homo sapiens activin A receptor type 2A (ACVR2A), transcript variant 3, mRNA.

Source:
NCBI, updated 2019-08-02
Taxon:
Homo sapiens (human)
Gene:
ACVR2A (92)
Length:
5177
CDS:
397..1614

Additional Resources:

NCBI RefSeq record:
NM_001278580.1
NBCI Gene record:
ACVR2A (92)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001487105 CCAGTTGCTTAACGAATATG pXPR_003 TGG 319 26% 5 0.4846 ACVR2A ACVR2A 77930
2 BRDN0001148932 AGTGGTTTCAAACCTAGTAA pXPR_003 TGG 234 19% 5 0.0948 ACVR2A ACVR2A 77929
3 BRDN0001148374 ATTGCAGAAACCATGGCTAG pXPR_003 AGG 566 46% 7 -0.1183 ACVR2A ACVR2A 77931
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001278580.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000244977 GGTATTAGAGGGTGCTATAAA pLKO_005 1179 CDS 100% 15.000 21.000 N ACVR2A n/a
2 TRCN0000244978 TAACCAGTGTTCGGCTTTATT pLKO_005 4299 3UTR 100% 15.000 21.000 N ACVR2A n/a
3 TRCN0000244975 CCCAGTTGCTTAACGAATATG pLKO_005 698 CDS 100% 13.200 18.480 N ACVR2A n/a
4 TRCN0000244976 GGCTAGAGGATTGGCATATTT pLKO_005 960 CDS 100% 15.000 12.000 N ACVR2A n/a
5 TRCN0000244974 ACTGGTGTTGAACCGTGTTAT pLKO_005 205 5UTR 100% 13.200 10.560 N ACVR2A n/a
6 TRCN0000022661 GCTAGAGGATTGGCATATTTA pLKO.1 961 CDS 100% 15.000 10.500 N Acvr2a n/a
7 TRCN0000274494 GCTAGAGGATTGGCATATTTA pLKO_005 961 CDS 100% 15.000 10.500 N Acvr2a n/a
8 TRCN0000000556 CAGGAAGTTGTTGTGCATAAA pLKO.1 1357 CDS 100% 13.200 9.240 N ACVR2A n/a
9 TRCN0000196571 GCTATGAGACTTGTAACTTTA pLKO.1 2745 3UTR 100% 13.200 9.240 N ACVR2A n/a
10 TRCN0000196507 GATGAATACATGTTGCCATTT pLKO.1 1297 CDS 100% 10.800 7.560 N ACVR2A n/a
11 TRCN0000194991 CCTTCTCCATTACTAGGTTTG pLKO.1 619 CDS 100% 6.000 4.200 N ACVR2A n/a
12 TRCN0000000553 CCAGATGCAGAGACTAACAAA pLKO.1 1512 CDS 100% 5.625 3.938 N ACVR2A n/a
13 TRCN0000194990 CGTGTTATGGTGACAAAGATA pLKO.1 218 5UTR 100% 5.625 3.938 N ACVR2A n/a
14 TRCN0000022663 GAGGGCAATATGTGTAATGAA pLKO.1 388 5UTR 100% 5.625 3.938 N Acvr2a n/a
15 TRCN0000022662 CCTCCCAAAGAATCTAGTCTA pLKO.1 1591 CDS 100% 4.950 3.465 N Acvr2a n/a
16 TRCN0000000552 CCTTAAATGAACTACTGCTAT pLKO.1 1969 3UTR 100% 4.950 3.465 N ACVR2A n/a
17 TRCN0000000555 CTATTACAACATCCTGCTCTA pLKO.1 477 CDS 100% 4.050 2.835 N ACVR2A n/a
18 TRCN0000000554 GCTAATGTGGTCTCTTGGAAT pLKO.1 913 CDS 100% 4.950 2.970 N ACVR2A n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001278580.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_05768 pDONR223 100% 78.8% 78.9% None 0_1ins324;30G>A n/a
2 ccsbBroad304_05768 pLX_304 0% 78.8% 78.9% V5 0_1ins324;30G>A n/a
3 TRCN0000476792 GCATCAATGGAAATCAAAGACCTG pLX_317 33.3% 78.8% 78.9% V5 0_1ins324;30G>A n/a
4 ccsbBroadEn_14529 pDONR223 0% 78.8% 78.9% None 0_1ins324;30G>A n/a
5 ccsbBroad304_14529 pLX_304 0% 78.8% 78.9% V5 0_1ins324;30G>A n/a
6 TRCN0000469988 CAAGCGACGGGCTTTCGGACTAAA pLX_317 25.7% 78.8% 78.9% V5 0_1ins324;30G>A n/a
7 TRCN0000489398 TCACAGGTGCGAGCCCAGATTTCA pLX_317 21.5% 78.8% 78.9% V5 (not translated due to prior stop codon) 0_1ins324;30G>A n/a
8 TRCN0000489291 AGTTCGTTACTAGGGTGTGACGGC pLX_317 23.3% 78.7% 78.9% V5 0_1ins324;30G>A;1214_1215delTA n/a
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