Construct: ORF TRCN0000469991
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018881.1_s317c1
- Derived from:
- ccsbBroadEn_14662
- DNA Barcode:
- GAACACTGGTACGACCTCTTACCG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- GTF2H1 (2965)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469991
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2965 | GTF2H1 | general transcription facto... | NM_001142307.1 | 98.5% | 43.5% | (many diffs) |
| 2 | human | 2965 | GTF2H1 | general transcription facto... | NM_005316.4 | 98.5% | 43.5% | (many diffs) |
| 3 | human | 2965 | GTF2H1 | general transcription facto... | XM_006718208.3 | 98.5% | 43.5% | (many diffs) |
| 4 | human | 2965 | GTF2H1 | general transcription facto... | XM_024448457.1 | 98.5% | 43.5% | (many diffs) |
| 5 | human | 2965 | GTF2H1 | general transcription facto... | XM_024448458.1 | 98.5% | 43.5% | (many diffs) |
| 6 | mouse | 14884 | Gtf2h1 | general transcription facto... | NM_008186.4 | 90.5% | 43% | (many diffs) |
| 7 | mouse | 14884 | Gtf2h1 | general transcription facto... | NM_001291075.1 | 89.6% | 42.5% | (many diffs) |
| 8 | mouse | 14884 | Gtf2h1 | general transcription facto... | XM_006540636.3 | 89.6% | 42.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 885
- ORF length:
- 816
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcaacctca tctgaagaag ttttgctgat tgtaaagaaa gtgcgtcaaa 121 agaagcagga tggagctctg tacctcatgg cagaaagaat tgcttgggca cctgaaggca 181 aagatagatt tacaatcagc catatgtatg cagatattaa atgccagaaa attagtccag 241 aaggaaaagc taaaattcag cttcagctgg tcctacatgc aggggacaca actaacttcc 301 atttttccaa tgaaagcaca gcagtgaaag agcgagatgc agtaaaagac cttcttcagc 361 agctgctgcc caaattcaag aggaaagcaa ataaagaact ggaagagaag aacagaatgc 421 tgcaagaaga tcctgttttg tttcagcttt ataaagacct tgttgtgagt caagtgatca 481 gtgctgagga attctgggcc aatcgtttaa atgtgaatgc aacagatagt tcttccacat 541 ccaatcataa gcaggatgtt ggcatttctg ctgcatttct ggctgatgtc cggccccaaa 601 ctgatggctg taacggtcta agatataatt taacttctga tatcattgag tccatattta 661 ggacctatcc agcagtaaaa atgaaatatg cagaaaatgt tccccacaac atgacagaga 721 aagaattctg gacacgtttt ttccagtccc attattttca cagggatcgg ctgatacagg 781 tcaaaggatc tctttgcaga atgtgcatag atgaaaagct aaaacatggt ttcattagag 841 tgaaaaccac tactagatta acagcttgga agataaccat tagatgaggg ctatggcatt 901 tcctctgtgc catctgcttc caattcttaa tcccataaag agaaatagta atgctgccat 961 catcaagaga tttaaccatc acagtgccat ggtcctggca gctggactca gaaaacaaga 1021 agcacaaaat gaacaaacta gtgagcccag caacatggat ggaaattccg gagatgcaga 1081 ctgctttcag ccagcagtca aaagggcgaa attacaagag tccattgaat atgaagactt 1141 ggggaaaaat aattctgtaa aaacgattgc actaaacctc aagaagtcag ataggtatta 1201 tcatggtcca actccaatcc agtcactaca gtatgcaaca agtcaggaca ttattaattc 1261 ttttcaaagt attagacaag aaatggaagc ttatacaccc aagttaactc aggttctctc 1321 aagtagtgct gccagtagta CCATCACAGC ACTGTCACCT GGAGGGGCAC TTATGCAGGG 1381 AGGAACACAG CAAGCCATAA ACCAGATGGT GCCAAATGAT ATTCAATCTG AATTGAAACA 1441 CTTATATGTA GCTGTTGGAG AACTTCTACG ACATTTCTGG TCCTGCTTTC CTGTTAATAC 1501 GCCATTCCTA GAAGAAAAGG TAGTGAAAAT GAAAAGTAAT TTGGAACGAT TCCAAGTTAC 1561 GAAGCTCTGT CCATTCCAAG AAAAGATTCG GAGACAGTAT TTAAGCACAA ATTTGGTAAG 1621 TCACATAGAA GAGATGCTCC AGACAGCCTA CAACAAGCTC CACACATGGC AGTCACGGCG 1681 TCTGATGAAG AAAACGTTGC CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT 1741 CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG 1801 TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGAACACTG GTACGACCTC 1861 TTACCGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga aagatt