Construct: ORF TRCN0000470021

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014817.1_s317c1
Derived from:
ccsbBroadEn_15775
DNA Barcode:
AGCGAGACGGGAACTCCTATTCAC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CNOT10 (25904)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000470021
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 25904 CNOT10 CCR4-NOT transcription comp... NM_001256741.2 99.9% 99.8% 2125C>T
2 human 25904 CNOT10 CCR4-NOT transcription comp... XM_017006112.2 98.2% 98% 1759_1760ins36;2089C>T
3 human 25904 CNOT10 CCR4-NOT transcription comp... XM_017006109.2 96.4% 96.2% 1515_1592del;2203C>T
4 human 25904 CNOT10 CCR4-NOT transcription comp... NM_015442.3 96.3% 96.1% 1515_1595del;2206C>T
5 human 25904 CNOT10 CCR4-NOT transcription comp... XM_017006111.2 94.8% 94.4% 1515_1592del;1837_1838ins36;2167C>T
6 human 25904 CNOT10 CCR4-NOT transcription comp... XM_017006110.2 94.7% 94.3% 1515_1595del;1840_1841ins36;2170C>T
7 human 25904 CNOT10 CCR4-NOT transcription comp... XM_011533566.2 91.7% 91.5% (many diffs)
8 human 25904 CNOT10 CCR4-NOT transcription comp... XM_006713084.3 88.7% 88.4% (many diffs)
9 human 25904 CNOT10 CCR4-NOT transcription comp... NM_001256742.1 88.6% 88.3% (many diffs)
10 human 25904 CNOT10 CCR4-NOT transcription comp... XM_006713085.3 87.1% 86.6% (many diffs)
11 human 25904 CNOT10 CCR4-NOT transcription comp... XR_001740089.1 73.4% (many diffs)
12 human 25904 CNOT10 CCR4-NOT transcription comp... NR_046352.2 71% (many diffs)
13 human 25904 CNOT10 CCR4-NOT transcription comp... XM_011533567.2 65.6% 65.4% 0_1ins684;831_911del;1522C>T
14 mouse 78893 Cnot10 CCR4-NOT transcription comp... XM_006512399.3 85.4% 92.5% (many diffs)
15 mouse 78893 Cnot10 CCR4-NOT transcription comp... NM_153585.5 85.3% 92.4% (many diffs)
16 mouse 78893 Cnot10 CCR4-NOT transcription comp... XM_017313699.1 83.7% 90.7% (many diffs)
17 mouse 78893 Cnot10 CCR4-NOT transcription comp... XR_871264.2 70.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2217
ORF length:
2151
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc tgcagacaag cctgcagatc agggagcaga gaaacatgaa ggcacaggtc
121agtcctctgg gatcactgat caagagaagg agttatccac caatgctttc caagctttca
181catctggaaa ttatgatgcc tgtctacaac accttgcctg tctacaagat ataaacaaag
241atgattataa aataattttg aatacagcag tagctgagtt ttttaaaagt aaccaaacaa
301caacagataa tttgagacaa acacttaacc agctgaagaa tcaggtccac tcagctgttg
361aagaaatgga tggattagat gatgttgaaa acagcatgtt gtactataat caagcagtca
421ttctttatca tctgcggcag tatacagaag ccatatcagt tggtgaaaaa ctttatcagt
481tcatagagcc ttttgaagaa aaatttgccc aagcagtgtg ttttttgctt gtagacctgt
541atatattaac ctaccaagct gagaaagctt tgcatcttct tgctgtccta gaaaaaatga
601tttcacaggg taacaataac aaaaatggaa agaatgagac tggtaataac aacaacaaag
661atggatctaa tcataaagct gaaagtggag ctctaataga agctgcaaaa tcaaagatac
721atcagtacaa agtacgagct tatatccaaa tgaagtctct gaaagcatgt aaaagggaaa
781tcaagtcagt catgaataca gctggaaatt ccgcaccctc tctctttctt aaaagcaatt
841ttgagtactt aagaggtaat tatcgaaaag ccgtgaagct attaaatagt tcaaacattg
901ctgagcatcc aggattcatg aaaacaggtg aatgcttgag atgcatgttc tggaataacc
961ttggttgcat ccattttgcc atgagcaagc acaatttggg aatattctac tttaaaaagg
1021ctctgcaaga gaatgacaat gtctgtgcac agctcagtgc aggtagcact gatccaggta
1081aaaaattttc aggaagaccc atgtgtacgt tactaaccaa taagagatat gagttgctgt
1141ataactgtgg aattcagctt cttcacattg gaaggcctct tgctgccttc gaatgtctga
1201ttgaagctgt tcaggtttat catgcaaatc ctcgcctctg gctacggctg gctgaatgct
1261gcattgctgc caataagggg acttctgaac aagaaactaa aggccttccc agcaaaaaag
1321gaattgtaca gtctattgtt ggtcaaggct atcatcgtaa aatagttttg gcatcacagt
1381ctatacagaa tactgtttat aatgatgggc agtcttcggc cattcctgta gccagtatgg
1441agtttgcagc catatgtctc agaaatgcct tgttgctgct acctgaagaa cagcaagatc
1501caaagcagga aaatggggct aaaaatagta atcaattagg tgggaacaca gagagcagcg
1561aaagcagtga aacttgcagg tgctccatac ttgcttgcag tgcctacgtg gctctggctt
1621tgggtgataa cctcatggct ttgaatcatg cagataaact tcttcagcag cccaagctgt
1681caggatctct taagtttttg ggacatttat atgctgcaga agccctcatc tctctcgaca
1741gaatatctga tgccattact cacttgaacc cggagaatgt cactgatgtc tccttaggga
1801tctcttcaaa tgagcaggac caaggatcag acaaaggtga aaatgaagca atggaatccT
1861CTGGTAAGCG GGCCCCTCAG TGCTACCCCA GTTCCGTCAA CTCTGCCAGG ACTGTGATGC
1921TGTTCAACCT TGGCAGCGCT TACTGCCTGA GGAGCGAATA TGACAAAGCC CGAAAGTGTC
1981TCCACCAGGC GGCTTCAATG ATCCATCCTA AAGAGGTGCC CCCTGAGGCC ATCTTGCTGG
2041CAGTCTACCT TGAACTGCAG AATGGTAATA CTCAGCTGGC CTTACAGATC ATCAAAAGGA
2101ATCAGCTGCT CCCTGCAGTG AAAACACACT CTGAAGTGAG AAAGAAGCCA GTGTTTCAGC
2161CTGTCCACCC GATCCAGCCC ATCCAAATGT CGGCTTTCAC CACTGTGCAG AGAAAGTGCC
2221CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC
2281TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT
2341ATATATCTTG TGGAAAGGAC GAAGCGAGAC GGGAACTCCT ATTCACACGC GTTAAGTCga
2401caatcaacct ctggattaca aaatttgtga aagatt

Download FASTA (ORF) (Full)