Construct: ORF TRCN0000470053
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013090.1_s317c1
- Derived from:
- ccsbBroadEn_04864
- DNA Barcode:
- GGCACGATCACTATGGCCTGGCGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GALM (130589)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470053
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 130589 | GALM | galactose mutarotase | NM_138801.3 | 100% | 100% | |
| 2 | human | 130589 | GALM | galactose mutarotase | XR_244925.2 | 63.3% | 1_240del;872_942del;1158_1159ins179 | |
| 3 | human | 130589 | GALM | galactose mutarotase | XM_011532540.2 | 62.7% | 62.2% | (many diffs) |
| 4 | mouse | 319625 | Galm | galactose mutarotase | NM_176963.4 | 85.5% | 87.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1092
- ORF length:
- 1026
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ttcggtgacc agggccgtgt ttggagagct gccctcggga ggagggacag 121 tggagaagtt ccagctgcag tcagacctct tgagagtgga catcatctcc tggggctgca 181 cgatcacagc cctagaggtc aaagacaggc aggggagagc ctcggacgtg gtgcttggct 241 tcgccgagtt ggaaggatac ctccaaaagc agccatactt tggagcagtt attgggaggg 301 tggccaaccg aatcgccaaa ggaaccttca aggtggatgg gaaggagtat cacctggcca 361 ttaacaagga acccaacagt ctgcatggag gagtcagagg gtttgataaa gtgctctgga 421 cccctcgggt gctgtcaaat ggcgtccagt tctcgcgcat cagtccagat ggtgaagaag 481 gctaccccgg agagttaaaa gtctgggtga catacaccct ggatggcgga gagctcatag 541 tcaactacag agcacaagcc agtcaggcca caccagtcaa cctgaccaac cattcttact 601 tcaacctggC AGGCCAGGCT TCCCCAAATA TAAATGACCA TGAAGTCACC ATAGAAGCGG 661 ATACTTATTT GCCTGTGGAT GAAACCCTGA TTCCTACAGG AGAAGTTGCC CCAGTGCAAG 721 GCACTGCATT CGACCTGAGA AAGCCAGTGG AGCTTGGAAA ACACCTGCAG GACTTCCATC 781 TCAATGGTTT TGACCACAAT TTCTGTCTGA AGGGATCTAA AGAAAAGCAT TTTTGTGCAA 841 GGGTGCATCA TGCTGCAAGC GGGCGGGTAC TAGAAGTATA CACCACCCAG CCCGGGGTCC 901 AGTTTTACAC GGGCAACTTC CTGGATGGCA CATTAAAGGG CAAGAATGGA GCTGTCTATC 961 CCAAGCACTC CGGTTTCTGC CTGGAGACTC AGAACTGGCC TGATGCAGTC AATCAGCCCC 1021 GCTTCCCTCC TGTGCTGCTG AGGCCTGGTG AGGAGTATGA CCACACCACC TGGTTCAAGT 1081 TTTCTGTGGC TTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1141 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1201 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAGGC ACGATCACTA TGGCCTGGCG 1261 CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t