Construct: ORF TRCN0000470066
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014243.1_s317c1
- Derived from:
- ccsbBroadEn_06435
- DNA Barcode:
- CCAATAGATCCTTCAAGCAGAACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RBPJ (3516)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470066
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3516 | RBPJ | recombination signal bindin... | NM_203283.3 | 99.9% | 100% | 189T>C |
| 2 | human | 3516 | RBPJ | recombination signal bindin... | NM_001374401.1 | 99.4% | 99.1% | (many diffs) |
| 3 | human | 3516 | RBPJ | recombination signal bindin... | NM_001374402.1 | 99.4% | 99.1% | (many diffs) |
| 4 | human | 3516 | RBPJ | recombination signal bindin... | NM_001374403.1 | 99.4% | 99.1% | (many diffs) |
| 5 | human | 3516 | RBPJ | recombination signal bindin... | NM_203284.2 | 99.4% | 99.1% | (many diffs) |
| 6 | human | 3516 | RBPJ | recombination signal bindin... | XM_011513840.3 | 99.4% | 99.1% | (many diffs) |
| 7 | human | 3516 | RBPJ | recombination signal bindin... | XM_017008174.2 | 99.4% | 99.1% | (many diffs) |
| 8 | human | 3516 | RBPJ | recombination signal bindin... | XM_017008175.2 | 99.4% | 99.1% | (many diffs) |
| 9 | human | 3516 | RBPJ | recombination signal bindin... | XM_024454032.1 | 99.4% | 99.1% | (many diffs) |
| 10 | human | 3516 | RBPJ | recombination signal bindin... | NM_015874.4 | 99.2% | 98.3% | (many diffs) |
| 11 | human | 3516 | RBPJ | recombination signal bindin... | NM_001374400.1 | 96.8% | 95.6% | (many diffs) |
| 12 | human | 3516 | RBPJ | recombination signal bindin... | NM_005349.3 | 96.8% | 95.6% | (many diffs) |
| 13 | human | 3516 | RBPJ | recombination signal bindin... | XM_017008171.2 | 95.8% | 96% | (many diffs) |
| 14 | human | 3516 | RBPJ | recombination signal bindin... | NM_001363577.2 | 95.8% | 95.8% | 0_1ins60;129T>C |
| 15 | human | 3516 | RBPJ | recombination signal bindin... | XM_017008170.2 | 95.5% | 95% | (many diffs) |
| 16 | mouse | 19664 | Rbpj | recombination signal bindin... | NM_001080928.1 | 90.3% | 98.3% | (many diffs) |
| 17 | mouse | 19664 | Rbpj | recombination signal bindin... | NM_001080927.2 | 90% | 97.3% | (many diffs) |
| 18 | mouse | 19664 | Rbpj | recombination signal bindin... | XM_006503797.3 | 89.4% | 97.3% | (many diffs) |
| 19 | mouse | 19664 | Rbpj | recombination signal bindin... | XM_006503799.2 | 86.8% | 94.8% | (many diffs) |
| 20 | mouse | 19664 | Rbpj | recombination signal bindin... | NM_001277116.1 | 86.4% | 94% | (many diffs) |
| 21 | mouse | 19664 | Rbpj | recombination signal bindin... | XM_006503796.3 | 83% | 89.7% | (many diffs) |
| 22 | mouse | 19664 | Rbpj | recombination signal bindin... | NM_009035.5 | 82.9% | 90.3% | (many diffs) |
| 23 | mouse | 19664 | Rbpj | recombination signal bindin... | XM_017320743.1 | 71.2% | 69.1% | (many diffs) |
| 24 | mouse | 19664 | Rbpj | recombination signal bindin... | XM_017320742.1 | 65.7% | 63.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1521
- ORF length:
- 1455
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gggctgcagg aaatttggtg agcggcctcc acctaaacga cttactaggg 121 aagctatgcg aaattattta aaagagcgag gggatcaaac agtacttatt cttcatgcaa 181 aagttgcaca gaagtcatat ggaaatgaaa aaaggttttt ttgcccacct ccttgtgtat 241 atcttatggg cagcggatgg aagaaaaaaa aagaacaaat ggaacgcgat ggttgttctg 301 aacaagagtc tcaaccgtgt gcatttattg ggataggaaa tagtgaccaa gaaatgcagc 361 agctaaactt ggaaggaaag aactattgca cagccaaaac attgtatata tctgactcag 421 acaagcgaaa gcacttcatg ttgtctgtaa agatgttcta tggcaacagt gatgacattg 481 gtgtgttcct cagcaagcgg ataaaagtca tctccaaacc ttccaaaaag aagcagtcat 541 tgaaaaatgc tgacttatgc attgcctcag gaacaaaggt ggctctgttt aatcgactac 601 gatcccagac agttagtacc agatacttgc atgtagaagg aggtaatttt catgccagtt 661 cacagcagtg gggagccttt tttattcatc tcttggatga tgatgaatca gaaggagaag 721 aattcacagt ccgagatggc tacatccatt atggacaaac agtcaaactt gtgtgctcag 781 ttactggcat ggcactccca agattgataa ttaggaaagt tgataagcag accgcattat 841 tggatgcaga tgatcctgtg tcacaactcc ataaatgtgc attttacctt aaggatacag 901 aaagaatgta tttgtgcctt tctcaagaaa gaataattca atttcaggcc actccatgtc 961 caaaagaacc aaataaagag atgataaatg atggcgcttc ctggacaatc attagcacag 1021 ataaggcaga gtatacattt tatgagggaa tgggccctgt ccttgcccca gtcactcctg 1081 tgcctgtggt agagagcctt CAGTTGAATG GCGGTGGGGA CGTAGCAATG CTTGAACTTA 1141 CAGGACAGAA TTTCACTCCA AATTTACGAG TGTGGTTTGG GGATGTAGAA GCTGAAACTA 1201 TGTACAGGTG TGGAGAGAGT ATGCTCTGTG TCGTCCCAGA CATTTCTGCA TTCCGAGAAG 1261 GTTGGAGATG GGTCCGGCAA CCAGTCCAGG TTCCAGTAAC TTTGGTCCGA AATGATGGAA 1321 TCATTTATTC CACCAGCCTT ACCTTTACCT ACACACCAGA ACCAGGGCCG CGGCCACATT 1381 GCAGTGCAGC AGGAGCAATC CTTCGAGCCA ATTCAAGCCA GGTGCCCCCT AACGAATCAA 1441 ACACAAACAG CGAGGGAAGT TACACAAACG CCAGCACAAA TTCAACCAGT GTCACATCAT 1501 CTACAGCCAC AGTGGTATCC TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1561 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1621 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACCAA TAGATCCTTC 1681 AAGCAGAACC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt