Construct: ORF TRCN0000470138
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012000.1_s317c1
- Derived from:
- ccsbBroadEn_03530
- DNA Barcode:
- GGAGTTGCTAGATCGACCTACCCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RBM28 (55131)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470138
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55131 | RBM28 | RNA binding motif protein 28 | NM_018077.3 | 100% | 100% | |
| 2 | human | 55131 | RBM28 | RNA binding motif protein 28 | XM_011516370.3 | 95.7% | 95.7% | 117_218del |
| 3 | human | 55131 | RBM28 | RNA binding motif protein 28 | XM_017012390.1 | 90.1% | 90.1% | 1562_1563ins225 |
| 4 | human | 55131 | RBM28 | RNA binding motif protein 28 | XM_017012389.1 | 86.2% | 86.2% | 117_218del;1664_1665ins225 |
| 5 | human | 55131 | RBM28 | RNA binding motif protein 28 | NM_001166135.2 | 81.4% | 81.4% | 113_114ins423 |
| 6 | human | 55131 | RBM28 | RNA binding motif protein 28 | XR_001744830.1 | 24.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2343
- ORF length:
- 2277
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cggcctgacc ttatttgtgg gccgcctccc gccctcggcc cgcagtgagc 121 agctggagga actgttcagt caggtggggc cggtgaagca gtgcttcgtg gtgactgaaa 181 aagggagtaa ggcatgtcga ggctttggct atgtcacttt ttcaatgctg gaagatgttc 241 agagggccct caaggagatt accacctttg aaggttgcaa gatcaacgtg actgttgcca 301 agaaaaaact gaggaacaag acaaaggaaa aggggaaaaa tgaaaactca gagtgcccaa 361 agaaggagcc gaaggctaaa aaagccaaag tggcagataa gaaagccaga ttaattattc 421 ggaacctgag ctttaagtgt tcagaagatg acttgaagac agtatttgct caatttggag 481 ctgtcctgga agtaaatatc cctaggaaac cagatgggaa gatgcgcggt tttggttttg 541 ttcagttcaa aaacctccta gaagcaggta aagctctcaa aggcatgaac atgaaagaga 601 taaaaggccg gacagtggct gtggattggg ccgtggcaaa ggataaatat aaagatacac 661 agtctgtttc tgctataggt gaggaaaaga gccatgaatc taaacatcag gaatcagtta 721 aaaagaaggg cagagaggaa gaggatatgg aagaggaaga aaacgatgat gatgacgatg 781 atgatgatga agaagatggg gtttttgatg atgaagatga agaggaagag aatatagaat 841 caaaggtgac caagcctgtg caaattcaga agagagcagt caagagacca gcccctgcaa 901 aaagcagtga tcattctgag gaggacagtg acctagagga aagcgatagt attgatgatg 961 gagaggaact ggctcagagt gataccagca ctgaggagca agaggataaa gctgtgcaag 1021 tctcaaacaa aaagaagagg aaattaccct ctgatgtgaa tgaagggaaa actgttttta 1081 tcagaaatct gtcctttgac tcagaagaag aagaacttgg ggagcttctc caacagtttg 1141 gagaactcaa atatgtccgc attgtcttgc atccagacac agagcattct aaaggttgtg 1201 catttgccca gttcatgact caagaagcag ctcagaaatg ccttctagct gcttctccag 1261 agaatgaggc tggtgggctt aaactggatg gccggcagct caaggttgac ttggcggtga 1321 cccgtgatga ggctgcaaag cttcagacga cgaaggtgaa gaagccgact ggcacccgga 1381 atctctatct ggcccgagaa ggcttgattc gtgctgggac gaaggctgca gagggtgtga 1441 gtgctgctga tatggccaaa agagaacggt ttgagctgct gaagcatcag aaactcaagg 1501 accagaatat ctttgtctcc cgaaccaggc tctgcctgca caatctccca aaggctgtag 1561 atgacaaaca gctcagaaag ctgctgctga gtgctactag tggagagaaa ggggtgcgca 1621 tcaaggagtg tagagtgatg cgagacctca aaggagttca tgggaacatg aagggtcagt 1681 ccctgggcta cgcctttgcg gagttccaag agcacgagca tgccctgaaa gccctccgcc 1741 tcatcaacaa caatccagaa atctttgggc ctctgaagag accaatagtg gagttctctt 1801 tagaagatcg aagaaaactt aaaatgaagg aattaaggat ccagcgcagc ttgcaaaaaa 1861 tgagatccaa gcctgcaact ggtgagcctc agaaggggca accagagcct gcaaaagacc 1921 agcaacagaa ggcagctcaa caCCACACAG AGGAACAAAG CAAGGTGCCC CCAGAGCAGA 1981 AGAGAAAGGC GGGCTCTACC TCATGGACCG GGTTCCAGAC CAAGGCTGAA GTGGAGCAGG 2041 TGGAGCTGCC TGATGGAAAG AAGAGAAGAA AGGTCCTGGC GCTCCCCTCA CACCGAGGCC 2101 CCAAAATCAG GTTGCGGGAC AAAGGCAAAG TGAAGCCCGT CCATCCCAAA AAGCCAAAGC 2161 CACAGATAAA CCAGTGGAAG CAGGAGAAGC AGCAATTATC GTCCGAGCAG GTATCTAGGA 2221 AAAAAGCTAA GGGAAATAAG ACGGAAACCC GCTTCAACCA GCTGGTCGAA CAATATAAGC 2281 AGAAATTATT GGGACCTTCT AAAGGAGCAC CTCTTGCAAA GAGGAGCAAA TGGTTTGATA 2341 GTTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 2401 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 2461 GGCTTTATAT ATCTTGTGGA AAGGACGAGG AGTTGCTAGA TCGACCTACC CGACGCGTTA 2521 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt