Construct: ORF TRCN0000470196
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014688.1_s317c1
- Derived from:
- ccsbBroadEn_04325
- DNA Barcode:
- CGTCAGGCTCCCCTGTCCCTCTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C3orf20 (84077)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470196
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84077 | C3orf20 | chromosome 3 open reading f... | NM_001184957.2 | 100% | 100% | |
| 2 | human | 84077 | C3orf20 | chromosome 3 open reading f... | NM_001184958.2 | 100% | 100% | |
| 3 | human | 84077 | C3orf20 | chromosome 3 open reading f... | XM_011534154.3 | 94.7% | 94.7% | 2129_2257del |
| 4 | human | 84077 | C3orf20 | chromosome 3 open reading f... | NM_032137.5 | 86.5% | 86.5% | 1_366del |
| 5 | human | 84077 | C3orf20 | chromosome 3 open reading f... | XM_006713341.2 | 82.5% | 82.5% | 1_366del;2495_2623del |
| 6 | human | 84077 | C3orf20 | chromosome 3 open reading f... | XM_006713342.2 | 82.5% | 82.5% | 1_366del;2495_2623del |
| 7 | human | 84077 | C3orf20 | chromosome 3 open reading f... | XM_011534153.3 | 82.5% | 82.5% | 1_366del;2495_2623del |
| 8 | human | 84077 | C3orf20 | chromosome 3 open reading f... | XM_011534157.2 | 72.7% | 68.7% | 0_1ins400;112_113ins146;1583_1711del |
| 9 | human | 84077 | C3orf20 | chromosome 3 open reading f... | XM_006713343.2 | 60.5% | 58% | 1_366del;1933_1934ins304;2007_2008ins401 |
| 10 | human | 84077 | C3orf20 | chromosome 3 open reading f... | XM_017007310.1 | 47.5% | 43.6% | (many diffs) |
| 11 | human | 84077 | C3orf20 | chromosome 3 open reading f... | XM_011534158.2 | 46.4% | 44.1% | 1_366del;1565_1566ins124;1626_1627ins962 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2412
- ORF length:
- 2346
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ccgtcaggtg cgcacccacc aggagaccct gaacaggttt cagcagcagt 121 ccatccacct gctgacggag ctcctcagac tgaagatgaa ggccatggtg gagtctatgt 181 cggtgggtgc caaccccttg gacatcacca ggcgctttgt ggaggccagc cagctcctcc 241 acctcaatgc caaggagatg gccttcaact gcctgatcag cacagccggg agaagtggct 301 acagcagcgg acagttgtgg aaagagtccc tcgcaaacat gtccgccatt ggggtgaact 361 cgccttacca gctgatctac cactcttcca cagcctgtct gagcttttct ctctctgctg 421 gaaaagaagc caagaagaaa ataggcaaat ctagaactac agaagatgtc agcatgccgc 481 ccctgcatcg aggagtggga acccctgcca acagcctgga gttcagcgac ccctgccctg 541 aggcccggga gaagctgcag gagttgtgtc gccacataga agctgaaagg gccacatgga 601 aagggaggaa tatctcctac cccatgatct tacgaaacta caaggcaaag atgccctctc 661 atctaatgtt ggcccgcaaa ggagactctc agaccccggg tttacattac cctcccactg 721 caggtgctca gactctcagc cccacctctc acccatcttc tgccaaccat catttcagtc 781 agcattgtca agaggggaag gcacccaaga aggccttcaa gtttcattac accttctatg 841 atggctcctc cttcgtttac tatccctctg gaaacgtcgc tgtatgtcag atccccacat 901 gctgcagagg gagaaccatc acctgcctct ttaatgacat acctggattc tccttgctgg 961 ccctattcaa tactgaaggc cagggctgtg ttcactacaa cctaaaaacc agttgcccat 1021 atgtcttaat cttggatgag gaaggtggga ccaccaatga ccagcagggc tatgtagtcc 1081 acaagtggag ctggacttcc aggacagaga ccctgctttc cctggaatac aaggtgaatg 1141 aggaaatgaa actaaaggta ctgggacagg actccatcac agtcaccttc acctccctga 1201 atgagacagt aacactcact gtgtcggcca acaattgtcc ccatggaatg gcatatgaca 1261 aacggctgaa ccgcagaatc agcaacatgg acgacaaggt gtataagatg agccgagccc 1321 tggctgagat caagaagcgg tttcagaaga cagtgactca gttcattaat tctatcttgc 1381 tggccgcagg tctgtttacc attgaatatc ccaccaaaaa ggaggaggaa gaatttgttc 1441 ggttcaagat gagatccaga actcatcccg agcggctccc caagctaagt ttatactcag 1501 gagaaagtct tttacgatct cagtcaggcc acctggaatc ctcaattgca gagactttga 1561 aggatgagcc tgagtctgct cctgtgagcc cagttcggaa gaccaccaaa atccacacca 1621 aagccaaggt cacatccaga gggaaggccc gcgaggggcg cagccccacc aggtgggcgg 1681 ccttgccctc agactgcccg ctggtgctgc ggaagctcat gctcaaggaa gacacccgtg 1741 ctggctgcaa gtgcctggtg aaggcgcccc tggtctctga cgtggagctg gagcgcttcc 1801 tgttggcgcc ccgagacccc agccaagtgc tggtgtttgg gatcatctca agccagaact 1861 acaccagcac tgggcagctc cagtggctgc tgaacactct ctacaaccac cagcagcggg 1921 gccgtggctc cccctgcatc cagtgccggt atgactccta ccgcctgctg cagtatgacc 1981 tggacagccc cctgcaggag gaccctcccc tgatggtgaa gaagaactct gtggtgcagg 2041 ggatgattct gatgtttgcc ggggggaagc tcatttttgg gggccgtgtt ttgaatggat 2101 atggcctcag caagcagaat ctgctgaaac agaTCTTCCG GTCTCAACAG GATTACAAGA 2161 TGGGCTACTT CCTGCCGGAT GACTACAAAT TCAGTGTTCC CAACTCTGTC CTGAGCCTGG 2221 AGGATTCTGA ATCAGTCAAG AAAGCCGAGT CAGAAGATAT CCAAGGAAGC AGCTCCTCAT 2281 TGGCCCTGGA AGACTATGTG GAGAAGGAGT TATCTCTGGA GGCTGAGAAG ACAAGAGAGC 2341 CTGAAGTGGA GCTACATCCT CTCAGCAGGG ACAGCAAGAT AACTAGTTGG AAGAAGCAGG 2401 CCTCCAAGAA GTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 2461 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 2521 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACGT CAGGCTCCCC TGTCCCTCTA 2581 CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t