Construct: ORF TRCN0000470200

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008003.3_s317c1
Derived from:
ccsbBroadEn_11336
DNA Barcode:
GCACGCCCAACACTTTTACGTCTA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
LATS1 (9113)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000470200
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9113 LATS1 large tumor suppressor kina... NM_001270519.1 99.9% 100% 1446T>C
2 human 9113 LATS1 large tumor suppressor kina... XM_017011482.1 97.2% 97.1% (many diffs)
3 human 9113 LATS1 large tumor suppressor kina... XM_006715603.3 76.9% 76.4% (many diffs)
4 human 9113 LATS1 large tumor suppressor kina... XM_017011479.1 68.6% 68.1% (many diffs)
5 human 9113 LATS1 large tumor suppressor kina... XM_011536252.2 68.1% 67.6% (many diffs)
6 human 9113 LATS1 large tumor suppressor kina... XM_017011477.1 65.9% 65.4% (many diffs)
7 human 9113 LATS1 large tumor suppressor kina... NM_004690.4 60.3% 59.9% (many diffs)
8 human 9113 LATS1 large tumor suppressor kina... XM_017011474.1 58.6% 58.2% (many diffs)
9 human 9113 LATS1 large tumor suppressor kina... XM_024446583.1 58.6% 58.2% (many diffs)
10 human 9113 LATS1 large tumor suppressor kina... NM_001350340.2 50.8% 49.9% (many diffs)
11 human 9113 LATS1 large tumor suppressor kina... NM_001350339.2 49.4% 48.4% (many diffs)
12 human 9113 LATS1 large tumor suppressor kina... NM_001350392.2 35.6% 35.1% (many diffs)
13 human 9113 LATS1 large tumor suppressor kina... XM_017011480.1 34.6% 34.1% (many diffs)
14 human 9113 LATS1 large tumor suppressor kina... NR_073033.1 27.5% (many diffs)
15 mouse 16798 Lats1 large tumor suppressor XM_006512567.1 84.3% 87.5% (many diffs)
16 mouse 16798 Lats1 large tumor suppressor NM_010690.1 52.5% 54% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2136
ORF length:
2070
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgaa gaggagtgaa aagccagaag gatatagaca aatgaggcct aagacctttc
121ctgccagtaa ctatactgtc agtagccggc aaatgttaca agaaattcgg gaatccctta
181ggaatttatc taaaccatct gatgctgcta aggctgagca taacatgagt aaaatgtcaa
241ccgaagatcc tcgacaagtc agaaatccac ccaaatttgg gacgcatcat aaagccttgc
301aggaaattcg aaactctctg cttccatttg caaatgaaac aaattcttct cggagtactt
361cagaagttaa tccacaaatg cttcaagact tgcaagctgc tggatttgat gaggatatgg
421ttatacaagc tcttcagaaa actaacaaca gaagtataga agcagcaatt gaattcatta
481gtaaaatgag ttaccaagat cctcgacgag agcagatggc tgcagcagct gccagaccta
541ttaatgccag catgaaacca gggaatgtgc agcaatcagt taaccgcaaa cagagctgga
601aaggttctaa agaatcctta gttcctcaga ggcatggccc gccactagga gaaagtgtgg
661cctatcattc tgagagtccc aactcacaga cagatgtagg aagacctttg tctggatctg
721gtatatcagc atttgttcaa gctcacccta gcaacggaca gagagtgaac cccccaccac
781cacctcaagt aaggagtgtt actcctccac cacctccaag aggccagact ccccctccaa
841gaggtacaac tccacctccc ccttcatggg aaccaaactc tcaaacaaag cgctattctg
901gaaacatgga atacgtaatc tcccgaatct ctcctgtccc acctggggca tggcaagagg
961gctatcctcc accacctctc aacacttccc ccatgaatcc tcctaatcaa ggacagagag
1021gcattagttc tgttcctgtt ggcagacaac caatcatcat gcagagttct agcaaattta
1081actttccatc agggagacct ggaatgcaga atggtactgg acaaactgat ttcatgatac
1141accaaaatgt tgtccctgct ggcactgtga atcggcagcc accacctcca tatcctctga
1201cagcagctaa tggacaaagc ccttctgctt tacaaacagg gggatctgct gctccttcgt
1261catatacaaa tggaagtatt cctcagtcta tgatggtgcc aaacagaaat agtcataaca
1321tggaactata taacattagt gtacctggac tgcaaacaaa ttggcctcag tcatcttctg
1381ctccagccca gtcatccccg agcagtgggc atgaaatccc tacatggcaa cctaacatac
1441cagtgaggtc aaattctttt aataacccat taggaaatag agcaagtcac tctgctaatt
1501ctcagccttc cgctacaaca gtcactgcaa ttacaccagc tcctattcaa cagcctgtga
1561aaagtatgcg tgtattaaaa ccagagctac agactgcttt agcacctaca cacccttctt
1621ggataccaca gccaattcaa actgttcaac ccagtccttt tcctgaggga accgcttcaa
1681atgtgactgt gatgccacct gttgctgaag ctccaaacta tcaaggacca ccaccaccct
1741acccaaaaca tcTGCTGCAC CAAAACCCAT CTGTTCCTCC ATACGAGTCA ATCAGTAAGC
1801CTAGCAAAGA GGATCAGCCA AGCTTGCCCA AGGAAGATGA GAGTGAAAAG AGTTATGAAA
1861ATGTTGATAG TGGGGATAAA GAAAAGAAAC AGATTACAAC TTCACCTATT ACTGTTAGGA
1921AAAACAAGAA AGATGAAGAG CGAAGGGAAT CTCGTATTCA AAGTTATTCT CCTCAAGCAT
1981TTAAATTCTT TATGGAGCAA CATGTAGAAA ATGTACTCAA ATCTCATCAG CAGCGTCTAC
2041ATCGTAAAAA ACAATTAGAG AATGAAATGA TGCGGGTAAA ACCTTTTAAA ATGTCCATTT
2101TTATACTTAA TCATCTGTTT GCTTGGTGTT TATTTTACCC AACTTTCTTG TACAAAGTGG
2161TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA
2221CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG
2281AGCACGCCCA ACACTTTTAC GTCTAACGCG TTAAGTCgac aatcaacctc tggattacaa
2341aatttgtgaa agatt

Download FASTA (ORF) (Full)