Construct: ORF TRCN0000470230
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004049.1_s317c1
- Derived from:
- ccsbBroadEn_05405
- DNA Barcode:
- TACATATTTGGGTCAACAGTCTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RNF180 (285671)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470230
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 285671 | RNF180 | ring finger protein 180 | NM_178532.4 | 100% | 100% | |
| 2 | human | 285671 | RNF180 | ring finger protein 180 | XM_017009387.1 | 81.9% | 81.4% | (many diffs) |
| 3 | human | 285671 | RNF180 | ring finger protein 180 | NM_001323292.1 | 78.3% | 76.4% | (many diffs) |
| 4 | human | 285671 | RNF180 | ring finger protein 180 | XM_017009386.2 | 76.1% | 75.7% | (many diffs) |
| 5 | human | 285671 | RNF180 | ring finger protein 180 | NM_001113561.2 | 70.1% | 68.5% | (many diffs) |
| 6 | human | 285671 | RNF180 | ring finger protein 180 | XM_017009383.1 | 69.1% | 68.7% | (many diffs) |
| 7 | human | 285671 | RNF180 | ring finger protein 180 | XM_017009388.1 | 69.1% | 68.7% | (many diffs) |
| 8 | human | 285671 | RNF180 | ring finger protein 180 | XM_017009389.1 | 69.1% | 68.7% | (many diffs) |
| 9 | human | 285671 | RNF180 | ring finger protein 180 | XR_001742057.1 | 64.5% | (many diffs) | |
| 10 | human | 285671 | RNF180 | ring finger protein 180 | XR_001742056.2 | 63.1% | (many diffs) | |
| 11 | human | 285671 | RNF180 | ring finger protein 180 | XM_017009385.1 | 56.2% | 54.8% | (many diffs) |
| 12 | human | 285671 | RNF180 | ring finger protein 180 | XM_017009384.1 | 55.5% | 55.1% | (many diffs) |
| 13 | human | 285671 | RNF180 | ring finger protein 180 | NM_001323291.1 | 20.9% | 19.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1317
- ORF length:
- 1248
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaaaagaagc aaagaattga taactaaaaa tcatagtcaa gaggaaacaa 121 gtattcttcg ttgttggaaa tgtagaaaat gtatagcaag ctctggttgt tttatggagt 181 atcttgagaa tcaagtgatt aaggataaag atgattcagt tgatgctcaa aatatttgtc 241 atgtgtggca catgaatgta gaagcccttc cagaatggat aagctgccta atccaaaaag 301 cccagtggac agttggaaaa ctgaattgtc ctttctgtgg ggcccgttta gggggcttta 361 attttgtcag cactccaaaa tgttcctgtg gccagcttgc agctgtacat ctctccaaga 421 gccggactga ttatcagcca acacaggcag gcagactaat gagaccatca gtgaaatact 481 tgtcacatcc tagagttcag tcaggttgtg acaaggaagc tctgctgaca ggtggtggct 541 ctgaaaacag aaatcacagg cttttaaaca tggcccgaaa taataatgac cctggaagat 601 taacagaagc actctgcctg gaggtgcgac caacatattt tgagatgaag aacgaaaaac 661 tgctgtccaa agcatcagaa ccaaaatacc agctttttgt tccccagctt gtgactggca 721 gatgcgctac aagagctttt catagaaaat cacatagttt ggatctgaac atcagtgaga 781 aactgacttt attacccact ttatatgaaa tacatagtaa gactactgcc tattccagac 841 taaatgaaac acagcctatt gaccTTTCAG GCTTGCCTTT ACAATCTAGT AAAAATAGCT 901 ATTCCTTTCA GAATCCATCC AGTTTTGATC CTAGTATGCT GCTGCAAAGA TTTTCAGTGG 961 CCCCCCATGA GACCCAGACA CAAAGAGGAG GAGAATTTCA GTGTGGTCTA GAAGCTGCTT 1021 CAGTGTATTC TGACCATACT AATACTAACA ATCTGACTTT CCTGATGGAC CTGCCCTCAG 1081 CTGGCAGGAG CATGCCGGAG GCCTCAGACC AGGAAGAGCA CCTCTCCCCT CTGGACTTCC 1141 TGCACTCAGC CAATTTTTCA TTGGGCAGCA TTAATCAGAG GCTTAATAAG AGAGAAAGGA 1201 GCAAGTTGAA GAATCTAAGA AGGAAACAAC GAAGGCGTGA AAGATGGCTA CAGAAGCAGG 1261 GTAAATACTC AGGAGTGGGA TTGCTGGATC ATATGGTAAG TATATATTTA CTTATATTGC 1321 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1381 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1441 ATATATCTTG TGGAAAGGAC GATACATATT TGGGTCAACA GTCTTCACGC GTTAAGTCga 1501 caatcaacct ctggattaca aaatttgtga aagatt