Construct: ORF TRCN0000470237
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010057.1_s317c1
- Derived from:
- ccsbBroadEn_11130
- DNA Barcode:
- AACTTCTATATCTTTAAACCTCTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SH3GL3 (6457)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470237
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6457 | SH3GL3 | SH3 domain containing GRB2 ... | NM_003027.5 | 81.9% | 80.8% | (many diffs) |
| 2 | human | 6457 | SH3GL3 | SH3 domain containing GRB2 ... | XM_024450017.1 | 79.5% | 73.6% | 624_625ins104;687_688ins73 |
| 3 | human | 6457 | SH3GL3 | SH3 domain containing GRB2 ... | NM_001324182.1 | 79.2% | 77.5% | (many diffs) |
| 4 | human | 6457 | SH3GL3 | SH3 domain containing GRB2 ... | NM_001301109.1 | 78% | 75.3% | (many diffs) |
| 5 | human | 6457 | SH3GL3 | SH3 domain containing GRB2 ... | NM_001324183.1 | 78% | 75.3% | (many diffs) |
| 6 | human | 6457 | SH3GL3 | SH3 domain containing GRB2 ... | XM_011521889.1 | 78% | 75.3% | (many diffs) |
| 7 | human | 6457 | SH3GL3 | SH3 domain containing GRB2 ... | XM_011521891.2 | 74.8% | 67.2% | (many diffs) |
| 8 | human | 6457 | SH3GL3 | SH3 domain containing GRB2 ... | NM_001324184.1 | 71.8% | 70.7% | (many diffs) |
| 9 | human | 6457 | SH3GL3 | SH3 domain containing GRB2 ... | NM_001324186.1 | 71.8% | 70.7% | (many diffs) |
| 10 | human | 6457 | SH3GL3 | SH3 domain containing GRB2 ... | XM_011521892.2 | 67.9% | 65.8% | (many diffs) |
| 11 | human | 6457 | SH3GL3 | SH3 domain containing GRB2 ... | NM_001301108.1 | 62% | 61% | (many diffs) |
| 12 | human | 6457 | SH3GL3 | SH3 domain containing GRB2 ... | NM_001324185.1 | 62% | 61% | (many diffs) |
| 13 | human | 6457 | SH3GL3 | SH3 domain containing GRB2 ... | NM_001324187.1 | 62% | 61% | (many diffs) |
| 14 | human | 6457 | SH3GL3 | SH3 domain containing GRB2 ... | XM_017022486.2 | 62% | 61% | (many diffs) |
| 15 | human | 6457 | SH3GL3 | SH3 domain containing GRB2 ... | XR_001751373.1 | 47.3% | (many diffs) | |
| 16 | human | 6457 | SH3GL3 | SH3 domain containing GRB2 ... | NR_136714.1 | 43.1% | (many diffs) | |
| 17 | human | 6457 | SH3GL3 | SH3 domain containing GRB2 ... | XR_931880.1 | 42.9% | 1_411del;454_604del;1427_2010del | |
| 18 | human | 6457 | SH3GL3 | SH3 domain containing GRB2 ... | NR_136713.1 | 42.7% | 1_306del;490_491insCAGCATA;1164_1996del | |
| 19 | human | 6457 | SH3GL3 | SH3 domain containing GRB2 ... | XR_931879.2 | 42.4% | 1_411del;454_604del;1427_2037del | |
| 20 | human | 6457 | SH3GL3 | SH3 domain containing GRB2 ... | NR_125370.1 | 42.3% | 1_306del;1171_2040del | |
| 21 | human | 6457 | SH3GL3 | SH3 domain containing GRB2 ... | XR_001751372.1 | 42.3% | 1_411del;454_604del;1427_2041del | |
| 22 | human | 6457 | SH3GL3 | SH3 domain containing GRB2 ... | XR_001751375.1 | 41.4% | (many diffs) | |
| 23 | human | 6457 | SH3GL3 | SH3 domain containing GRB2 ... | XR_001751374.2 | 40.5% | (many diffs) | |
| 24 | human | 6457 | SH3GL3 | SH3 domain containing GRB2 ... | NR_136711.1 | 39.7% | (many diffs) | |
| 25 | human | 6457 | SH3GL3 | SH3 domain containing GRB2 ... | XR_931878.2 | 38.2% | 1_411del;454_604del;1427_2259del | |
| 26 | human | 6457 | SH3GL3 | SH3 domain containing GRB2 ... | XR_002957669.1 | 37.3% | (many diffs) | |
| 27 | human | 6457 | SH3GL3 | SH3 domain containing GRB2 ... | NR_136712.1 | 37% | 1_306del;350_680del;1502_2334del | |
| 28 | human | 6457 | SH3GL3 | SH3 domain containing GRB2 ... | XR_931882.2 | 33.6% | (many diffs) | |
| 29 | mouse | 20408 | Sh3gl3 | SH3-domain GRB2-like 3 | NM_017400.6 | 71% | 69.3% | (many diffs) |
| 30 | mouse | 20408 | Sh3gl3 | SH3-domain GRB2-like 3 | NM_001277954.1 | 53.4% | 51.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 930
- ORF length:
- 864
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc ggtggccggg ctgaagaagc agttccacaa agccagccag ctatttagtg 121 aaaaaataag tggtgctgaa ggaactaaac tagacgatga atttcttgac atggaaagga 181 aaatagatgt taccaataaa gttgttgcag aaattctttc aaaaaccact gaatatcttc 241 agccaaatcc agcatacaga gctaagctag gaatgctgaa cactgtgtcg aagatccgag 301 ggcaggtgaa gaccacagga tacccgcaga cggaaggctt gctgggggac tgtatgctga 361 aatacgggaa ggagctcggg gaagactcca cctttggcaa tgcattgata gaagttggtg 421 aatccatgaa gctaatggct gaggtgaaag actctcttga tattaatgta aagcaaactt 481 ttattgatcc acttcagtta ctacaagata aagatttaaa agagatcggg catcacctga 541 aaaagctggA AGGCCGCCGC CTGGATTACG ATTATAAAAA GAAACGAGTA GGTAAGATAC 601 CAGACGAAGA AGTCAGACAA GCGGTAGAAA AATTTGAAGA GTCAAAGGAG TTGGCTGAAA 661 GAAGCATGTT TAACTTTTTA GAAAATGATG TAGAACAAGT CAGCCAGTTG GCTGTGTTCA 721 TAGAGGCAGC ATTAGACTAT CACAGACAGT CCACAGAGAT TCTGCAGGAG CTGCAGAGCA 781 AGCTACAGAT GCGAATATCA GCTGCATCCA GTGTCCCCAG ACGAGAATAC AAGCCAAGGC 841 CTGTGAAAAG GAGTTCTAGT GAGCTCAATG GAGTTTCCAC CACCTCTGTA GTGAAGACGA 901 CAGCCTACAG CAGACTGGAA CCAGCAGATT ACCCAACTTT CTTGTACAAA GTGGTTGATA 961 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1021 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAAACTT 1081 CTATATCTTT AAACCTCTCA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1141 tgaaagatt