Construct: ORF TRCN0000470254
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008982.1_s317c1
- Derived from:
- ccsbBroadEn_11112
- DNA Barcode:
- CCCCTCTTGGAGGTTCAATCCCAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RTN1 (6252)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470254
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6252 | RTN1 | reticulon 1 | NM_206852.2 | 95.6% | 95.6% | 1_27del |
| 2 | human | 6252 | RTN1 | reticulon 1 | NM_001363702.1 | 93.8% | 94.9% | (many diffs) |
| 3 | human | 6252 | RTN1 | reticulon 1 | NM_021136.3 | 24.8% | 24.4% | (many diffs) |
| 4 | mouse | 104001 | Rtn1 | reticulon 1 | NM_001007596.2 | 87.5% | 94.2% | (many diffs) |
| 5 | mouse | 104001 | Rtn1 | reticulon 1 | NM_001286448.1 | 67% | 68.4% | (many diffs) |
| 6 | mouse | 104001 | Rtn1 | reticulon 1 | NR_104446.1 | 36.9% | (many diffs) | |
| 7 | mouse | 104001 | Rtn1 | reticulon 1 | XM_006515323.1 | 25.2% | 26.7% | (many diffs) |
| 8 | mouse | 104001 | Rtn1 | reticulon 1 | XM_006515324.1 | 25.2% | 26.7% | (many diffs) |
| 9 | mouse | 104001 | Rtn1 | reticulon 1 | NM_153457.7 | 22.6% | 23.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 663
- ORF length:
- 597
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ctgtgtgtgg agcaactgga aaagtcaggc tattgacctg ttgtattggc 121 gggacatcaa gcagacgggc atcgtgtttg ggagtttcct gctgctgctc ttctccctga 181 cccagttcag cgtggtgagc gtcgtggcct acctggccct ggccgcactc tcagccacca 241 tcagtttccg catctacaag tctgttttac aagcagtgca gaaaaccgac gaaggccacc 301 ctttcaaggc ctacttGGAG CTTGAGATCA CCCTTTCTCA GGAGCAGATT CAGAAGTACA 361 CGGACTGCCT GCAGTTCTAC GTGAACAGCA CACTTAAGGA ACTGAGGAGG CTCTTCCTTG 421 TCCAGGACCT GGTGGATTCC TTAAAATTTG CAGTCCTGAT GTGGCTCCTG ACCTACGTTG 481 GCGCTCTCTT CAATGGCCTG ACCCTGCTGC TCATGGCTGT GGTTTCAATG TTTACTCTAC 541 CTGTAGTGTA TGTTAAGCAC CAGGCACAGA TTGACCAATA TCTGGGACTT GTGAGGACTC 601 ACATAAATGC TGTTGTGGCA AAGATTCAGG CTAAAATCCC AGGCGCTAAG AGGCACGCTG 661 AGTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 721 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTCTTG 781 GCTTTATATA TCTTGTGGAA AGGACGACCC CTCTTGGAGG TTCAATCCCA TACGCGTTAA 841 GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t