Construct: ORF TRCN0000470278
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001858.1_s317c1
- Derived from:
- ccsbBroadEn_00668
- DNA Barcode:
- AGTCGACGGCATAAACCGTTCCGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GPM6B (2824)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470278
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2824 | GPM6B | glycoprotein M6B | NM_001001996.2 | 100% | 100% | |
| 2 | human | 2824 | GPM6B | glycoprotein M6B | XM_017029432.1 | 93.5% | 93.4% | 0_1ins57;2_3delTGinsAA |
| 3 | human | 2824 | GPM6B | glycoprotein M6B | NM_001001995.3 | 89.2% | 87.5% | (many diffs) |
| 4 | human | 2824 | GPM6B | glycoprotein M6B | NM_005278.4 | 86.8% | 86.8% | 59_60ins120 |
| 5 | human | 2824 | GPM6B | glycoprotein M6B | XM_011545497.2 | 83.3% | 81.4% | (many diffs) |
| 6 | human | 2824 | GPM6B | glycoprotein M6B | NM_001001994.3 | 80.2% | 80.3% | 0_1ins179;2_3delTG |
| 7 | human | 2824 | GPM6B | glycoprotein M6B | XM_005274489.5 | 77.2% | 75.3% | (many diffs) |
| 8 | human | 2824 | GPM6B | glycoprotein M6B | NM_001318729.1 | 71.1% | 69.2% | (many diffs) |
| 9 | mouse | 14758 | Gpm6b | glycoprotein m6b | NM_001177956.1 | 93.4% | 98% | (many diffs) |
| 10 | mouse | 14758 | Gpm6b | glycoprotein m6b | NM_001177957.1 | 93.4% | 98% | (many diffs) |
| 11 | mouse | 14758 | Gpm6b | glycoprotein m6b | XM_017318385.1 | 88.2% | 90.7% | (many diffs) |
| 12 | mouse | 14758 | Gpm6b | glycoprotein m6b | NM_001177955.1 | 83.6% | 85.6% | (many diffs) |
| 13 | mouse | 14758 | Gpm6b | glycoprotein m6b | NM_001177959.1 | 80.7% | 85.5% | (many diffs) |
| 14 | mouse | 14758 | Gpm6b | glycoprotein m6b | NM_001177960.1 | 80.7% | 85.5% | (many diffs) |
| 15 | mouse | 14758 | Gpm6b | glycoprotein m6b | XM_011247784.1 | 79% | 79.1% | (many diffs) |
| 16 | mouse | 14758 | Gpm6b | glycoprotein m6b | NM_001177961.1 | 74.1% | 79% | (many diffs) |
| 17 | mouse | 14758 | Gpm6b | glycoprotein m6b | NM_001177962.1 | 74.1% | 79% | (many diffs) |
| 18 | mouse | 14758 | Gpm6b | glycoprotein m6b | NM_001177958.1 | 71.9% | 74% | (many diffs) |
| 19 | mouse | 14758 | Gpm6b | glycoprotein m6b | NM_023122.3 | 65.9% | 67.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 981
- ORF length:
- 915
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gccagccatg gaaactgcag ccgaggaaaa tactgaacaa agccaagaga 121 gaaaagtgaa cagcagagct gaaatggaaa ttggcaggta ccactggatg tacccaggct 181 caaagaacca ccagtaccat cccgtgccaa ccctggggga cagggctagc cccttgagca 241 gtccaggctg ctttgaatgc tgcatcaagt gtctgggagg agtcccctac gcctccctgg 301 tggccaccat cctctgcttc tccggggtgg ccttattctg cggctgtggg catgtggctc 361 tcgcaggcac cgtggcgatt cttgagcaac acttctccac caacgccagt gaccatgcct 421 tgctgagcga ggtgatacaa ctgatgcagt atgtcatcta tggaattgcg tcctttttct 481 tcttgtatgg gatcattctg ttggcagaag gcttttacac cacaagtgca gtgaaagaac 541 tgcacggtga gtttaaaaca accgcttgtg gccgatgcat cagtggaatg ttcgttttcc 601 tcacctatgt gcttggagtg gcctggctgg gtgtgtttgg tttcTCAGCG GTGCCCGTGT 661 TTATGTTCTA CAACATATGG TCAACTTGTG AAGTCATCAA GTCACCGCAG ACCAACGGGA 721 CCACGGGTGT GGAGCAGATC TGTGTGGATA TCCGACAATA CGGTATCATT CCTTGGAATG 781 CTTTCCCCGG AAAAATATGT GGCTCTGCCC TGGAGAACAT CTGCAACACA AACGAGTTCT 841 ACATGTCCTA TCACCTGTTC ATTGTGGCCT GTGCAGGAGC TGGTGCCACC GTCATTGCCC 901 TGCTGATCTA CATGATGGCT ACTACATATA ACTATGCGGT TTTGAAGTTT AAGAGTCGGG 961 AAGATTGCTG CACTAAATTC TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1021 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1081 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAAGTC GACGGCATAA 1141 ACCGTTCCGG ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt