Construct: ORF TRCN0000470322

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015971.1_s317c1
Derived from:
ccsbBroadEn_02356
DNA Barcode:
TTAATGTTGTCGGCCGCCTCAATT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DDX39A (10212)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000470322
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 10212 DDX39A DExD-box helicase 39A NM_005804.4 100% 100%
2 human 10212 DDX39A DExD-box helicase 39A XM_011527620.1 100% 100%
3 human 7919 DDX39B DExD-box helicase 39B NM_004640.7 78.6% 89.9% (many diffs)
4 human 7919 DDX39B DExD-box helicase 39B NM_080598.6 78.6% 89.9% (many diffs)
5 human 10212 DDX39A DExD-box helicase 39A NR_046366.2 75.1% 1_118del;981_982ins110;1290_1448del
6 human 10212 DDX39A DExD-box helicase 39A XM_024451310.1 59.4% 53.8% (many diffs)
7 human 10212 DDX39A DExD-box helicase 39A XM_006722606.4 51.9% 51.9% 0_1ins615
8 human 10212 DDX39A DExD-box helicase 39A XM_011527621.3 51.9% 51.9% 0_1ins615
9 human 10212 DDX39A DExD-box helicase 39A XM_024451311.1 51.9% 51.9% 0_1ins615
10 human 10212 DDX39A DExD-box helicase 39A XM_024451312.1 51.9% 51.9% 0_1ins615
11 mouse 68278 Ddx39 DEAD (Asp-Glu-Ala-Asp) box ... NM_197982.3 88.9% 97.1% (many diffs)
12 mouse 68278 Ddx39 DEAD (Asp-Glu-Ala-Asp) box ... XM_006531332.3 88.9% 97.1% (many diffs)
13 mouse 68278 Ddx39 DEAD (Asp-Glu-Ala-Asp) box ... XM_017312949.1 82.9% 90.6% (many diffs)
14 mouse 68278 Ddx39 DEAD (Asp-Glu-Ala-Asp) box ... XM_017312950.1 82.9% 90.6% (many diffs)
15 mouse 53817 Ddx39b DEAD (Asp-Glu-Ala-Asp) box ... NM_001252457.1 80.9% 90.1% (many diffs)
16 mouse 53817 Ddx39b DEAD (Asp-Glu-Ala-Asp) box ... NM_019693.3 80.9% 90.1% (many diffs)
17 mouse 68278 Ddx39 DEAD (Asp-Glu-Ala-Asp) box ... XM_017312952.1 47.1% 51.2% (many diffs)
18 mouse 68278 Ddx39 DEAD (Asp-Glu-Ala-Asp) box ... XM_017312951.1 44% 47.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1347
ORF length:
1281
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc agaacaggat gtggaaaacg atcttttgga ttacgatgaa gaggaagagc
121cccaggctcc tcaagagagc acaccagctc cccctaagaa agacatcaag ggatcctacg
181tttccatcca cagctctggc ttccgggact ttctgctgaa gccggagctc ctgcgggcca
241tcgtggactg tggctttgag catccttctg aggtccagca tgagtgcatt ccccaggcca
301tcctgggcat ggacgtcctg tgccaggcca agtccgggat gggcaagaca gcggtcttcg
361tgctggccac cctacagcag attgagcctg tcaacggaca ggtgacggtc ctggtcatgt
421gccacacgag ggagctggcc ttccagatca gcaaggaata tgagcgcttt tccaagtaca
481tgcccagcgt caaggtgtct gtgttcttcg gtggtctctc catcaagaag gatgaagaag
541tgttgaagaa gaactgtccc catgtcgtgg tggggacccc gggccgcatc ctggcgctcg
601tgcggaatag gagcttcagc ctaaagaatg tgaagcactt tgtgctggac gagtgtgaca
661agatgctgga gcagctggac atgcggcggg atgtgcagga gatcttccgc ctgacaccac
721acgagaagca gtgcatgatg ttcagcgcca ccctgagcaa ggacatccgg cctgtgtgca
781ggaagttcat gcaggatccc atggaggtgt ttgtggacga cgagaccaag ctcacgctgc
841acggcctgca gcagtactac gtcaaactca aagacagtga gaagaaccgc aagctctttg
901atctcttgga tgtgctggag tttaaccagg tgataatctt cgtcaagtca gtgcagcgct
961gcatggccct ggcccagctc cTCGTGGAGC AGAACTTCCC GGCCATCGCC ATCCACCGGG
1021GCATGGCCCA GGAGGAGCGC CTGTCACGCT ATCAGCAGTT CAAGGATTTC CAGCGGCGGA
1081TCCTGGTGGC CACCAATCTG TTTGGCCGGG GGATGGACAT CGAGCGAGTC AACATCGTCT
1141TTAACTACGA CATGCCTGAG GACTCGGACA CCTACCTGCA CCGGGTGGCC CGGGCGGGTC
1201GCTTTGGCAC CAAAGGCCTA GCCATCACTT TTGTGTCTGA CGAGAATGAT GCCAAAATCC
1261TCAATGACGT CCAGGACCGG TTTGAAGTTA ATGTGGCAGA ACTTCCAGAG GAAATCGACA
1321TCTCCACATA CATCGAGCAG AGCCGGTACC CAACTTTCTT GTACAAAGTG GTTGATATCG
1381GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT
1441AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATTAATGTT
1501GTCGGCCGCC TCAATTACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga
1561aagatt

Download FASTA (ORF) (Full)