Construct: ORF TRCN0000470375
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007160.1_s317c1
- Derived from:
- ccsbBroadEn_07719
- DNA Barcode:
- CCTGTACGACGGTCTCAATCCCGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IMMT (10989)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470375
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10989 | IMMT | inner membrane mitochondria... | NM_006839.2 | 99.8% | 99.8% | 922G>A;1464T>C;1785A>G |
| 2 | human | 10989 | IMMT | inner membrane mitochondria... | XM_017003194.2 | 99.8% | 99.8% | 922G>A;1464T>C;1785A>G |
| 3 | human | 10989 | IMMT | inner membrane mitochondria... | NM_001100169.1 | 99.7% | 99.7% | (many diffs) |
| 4 | human | 10989 | IMMT | inner membrane mitochondria... | NM_001100170.1 | 98.4% | 98.4% | (many diffs) |
| 5 | human | 10989 | IMMT | inner membrane mitochondria... | XM_005264110.4 | 98.2% | 98.2% | (many diffs) |
| 6 | human | 10989 | IMMT | inner membrane mitochondria... | XM_005264113.2 | 95.6% | 95.5% | (many diffs) |
| 7 | human | 10989 | IMMT | inner membrane mitochondria... | XM_005264114.2 | 94.1% | 94% | (many diffs) |
| 8 | human | 10989 | IMMT | inner membrane mitochondria... | XM_011532497.3 | 60.9% | 60.5% | (many diffs) |
| 9 | human | 10989 | IMMT | inner membrane mitochondria... | XM_017003195.2 | 60.9% | 60.5% | (many diffs) |
| 10 | mouse | 76614 | Immt | inner membrane protein, mit... | NM_029673.3 | 87.7% | 89.9% | (many diffs) |
| 11 | mouse | 76614 | Immt | inner membrane protein, mit... | XM_017321823.1 | 87.2% | 89.3% | (many diffs) |
| 12 | mouse | 76614 | Immt | inner membrane protein, mit... | NM_001253681.1 | 86.5% | 88.7% | (many diffs) |
| 13 | mouse | 76614 | Immt | inner membrane protein, mit... | XM_017321824.1 | 86.3% | 88.6% | (many diffs) |
| 14 | mouse | 76614 | Immt | inner membrane protein, mit... | NM_001253686.1 | 85.9% | 88.1% | (many diffs) |
| 15 | mouse | 76614 | Immt | inner membrane protein, mit... | XM_017321825.1 | 84.4% | 86.4% | (many diffs) |
| 16 | mouse | 76614 | Immt | inner membrane protein, mit... | XM_017321826.1 | 83.9% | 85.7% | (many diffs) |
| 17 | mouse | 76614 | Immt | inner membrane protein, mit... | XM_017321827.1 | 83.1% | 85.2% | (many diffs) |
| 18 | mouse | 76614 | Immt | inner membrane protein, mit... | XM_017321828.1 | 83% | 85% | (many diffs) |
| 19 | mouse | 76614 | Immt | inner membrane protein, mit... | NM_001253687.1 | 82.6% | 84.5% | (many diffs) |
| 20 | mouse | 76614 | Immt | inner membrane protein, mit... | NM_001253688.1 | 79.5% | 82.1% | (many diffs) |
| 21 | mouse | 76614 | Immt | inner membrane protein, mit... | NM_001253689.1 | 54.6% | 55% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2340
- ORF length:
- 2274
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct gcgggcctgt cagttatcgg gtgtgaccgc cgccgcccag agttgtctct 121 gtgggaagtt tgtcctccgt ccattgcgac catgccgcag atactctact tcaggcagct 181 ctgggttgac tactggcaaa attgctggag ctggcctttt gtttgttggt ggaggtattg 241 gtggcactat cctatatgcc aaatgggatt cccatttccg ggaaagtgta gagaaaacca 301 taccttactc agacaaactc ttcgagatgg ttcttggtcc tgcagcttat aatgttccat 361 tgccaaagaa atcgattcag tcgggtccac taaaaatctc tagtgtatca gaagtaatga 421 aagaatctaa acagcctgcc tcacaactcc aaaaacaaaa gggagatact ccagcttcag 481 caacagcacc tacagaagcg gctcaaatta tttctgcagc aggtgatacc ctgtcggtcc 541 cagcccctgc agttcagcct gaggaatctt taaaaactga tcaccctgaa attggtgaag 601 gaaaacccac acctgcactt tcagaagaag catcctcatc ttctataagg gagcgaccac 661 ctgaagaagt tgcagctcgc cttgcacaac aggaaaaaca agaacaagtt aaaattgagt 721 ctctagccaa gagcttagaa gatgctctga ggcaaactgc aagtgtcact ctgcaggcta 781 ttgcagctca gaatgctgcg gtccaggctg tcaatgcaca ctccaacata ttgaaagccg 841 ccatggacaa ttctgagatt gcaggcgaga agaaatctgc tcagtggcgc acagtggagg 901 gtgcattgaa ggaacgcaga aaggcagtag atgaagctgc cgatgccctt ctcaaagcca 961 aagaagagtt agagaagatg aaaagtatga ttgaaaatgc aaagaaaaaa gaggttgctg 1021 gggccaagcc tcatataact gctgcagagg gtaaacttca caacatgata gttgatctgg 1081 ataatgtggt caaaaaggtc caagcagctc agtctgaggc taaggttgta tctcagtatc 1141 atgagctggt ggtccaagct cgggatgact ttaaacgaga gctggacagt attactccag 1201 aagtccttcc tgggtggaaa ggaatgagtg tttcagactt agctgacaag ctctctactg 1261 atgatctgaa ctccctcatt gctcatgcac atcgtcgtat tgatcagctg aacagagagc 1321 tggcagaaca gaaggccacc gaaaagcagc acatcacgtt agccttggag aaacaaaagc 1381 tggaagaaaa gcgggcattt gactctgcag tagcaaaagc attagaacat cacagaagtg 1441 aaatacaggc tgaacaggac agaaagatag aagaagtcag agatgccatg gaaaatgaaa 1501 tgagaaccca gcttcgccga caggcagccg cccacactga tcacttgcga gatgtcctta 1561 gggtacaaga acaggaattg aagtctgaat ttgagcagaa cctgtctgag aaactctctg 1621 aacaagaatt acaatttcgt cgtctcagtc aagagcaagt tgacaacttt actctggata 1681 taaatactgc ctatgccaga ctcagaggaa tcgaacaggc tgttcagagc catgcagttg 1741 ctgaagagga agccagaaaa gcccaccaac tctggctttc agtggaggca ttaaagtaca 1801 gcatgaagac ctcatctgca gaaacaccta ctatcccgct gggtagtgcg gttGAGGCCA 1861 TCAAAGCCAA CTGTTCTGAT AATGAATTCA CCCAAGCTTT AACCGCAGCT ATCCCTCCAG 1921 AGTCCCTGAC CCGTGGGGTG TACAGTGAAG AGACCCTTAG AGCCCGTTTC TATGCTGTTC 1981 AAAAACTGGC CCGAAGGGTA GCAATGATTG ATGAAACCAG AAATAGCTTG TACCAGTACT 2041 TCCTCTCCTA CCTACAGTCC CTGCTCCTAT TCCCACCTCA GCAACTGAAG CCGCCCCCAG 2101 AGCTCTGCCC TGAGGATATA AACACATTTA AATTACTGTC ATATGCTTCC TATTGCATTG 2161 AGCATGGTGA TCTGGAGCTA GCAGCAAAGT TTGTCAATCA GCTGAAGGGG GAATCCAGAC 2221 GAGTGGCACA GGACTGGCTG AAGGAAGCCC GAATGACCCT AGAAACGAAA CAGATAGTGG 2281 AAATCCTGAC AGCATATGCC AGCGCCGTAG GAATAGGAAC CACTCAGGTG CAGCCAGAGT 2341 GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 2401 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 2461 TTTATATATC TTGTGGAAAG GACGACCTGT ACGACGGTCT CAATCCCGCA CGCGTTAAGT 2521 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt