Construct: ORF TRCN0000470429
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013487.1_s317c1
- Derived from:
- ccsbBroadEn_04020
- DNA Barcode:
- TGTATACAGTGCAGACGAGCGACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SCNM1 (79005)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470429
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79005 | SCNM1 | sodium channel modifier 1 | NM_024041.4 | 100% | 100% | |
| 2 | human | 100534012 | TNFAIP8L2-SCNM1 | TNFAIP8L2-SCNM1 readthrough | NM_001204848.2 | 84.7% | 84.7% | 0_1ins105 |
| 3 | human | 79005 | SCNM1 | sodium channel modifier 1 | NM_001204856.2 | 84.7% | 84.7% | 0_1ins105 |
| 4 | human | 100534012 | TNFAIP8L2-SCNM1 | TNFAIP8L2-SCNM1 readthrough | NR_144937.2 | 34.3% | (many diffs) | |
| 5 | human | 79005 | SCNM1 | sodium channel modifier 1 | NR_037937.2 | 33.4% | 1_9delACAGTCGTG;404_405ins50;650_1863del | |
| 6 | mouse | 69269 | Scnm1 | sodium channel modifier 1 | NM_001163573.1 | 62.9% | 64.3% | (many diffs) |
| 7 | mouse | 69269 | Scnm1 | sodium channel modifier 1 | XM_017319715.1 | 53.5% | 52.1% | (many diffs) |
| 8 | mouse | 69269 | Scnm1 | sodium channel modifier 1 | XM_017319716.1 | 44.6% | 40.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 756
- ORF length:
- 690
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc tttcaagagg gaaggagacg attggagtca actcaatgtg ctcaaaaaaa 121 gaagagtcgg ggacctccta gccagttaca ttccagagga tgaggcgctg atgcttcggg 181 atggacgctt tgcttgtgcc atctgccccc atcgaccggt actggacacc ctggccatgc 241 tgactgccca ccgtgcaggc aagaaacatc tgtccagctt gcagcttttc tatggcaaga 301 agcagccggg aaaggaaaga aagcagaaTC CAAAACATCA GAATGAATTG AGAAGGGAAG 361 AAACCAAAGC TGAGGCTCCT CTGCTAACTC AGACACGACT TATCACCCAG AGTGCTCTGC 421 ACAGAGCTCC CCACTATAAC AGTTGCTGCC GCCGGAAGTA CAGACCAGAA GCCCCTGGTC 481 CCTCTGTCTC CCTTTCCCCT ATGCCACCCT CAGAGGTCAA ACTCCAAAGT GGGAAGATCA 541 GTAGGGAACC TGAACCTGCG GCTGGCCCAC AGGCCGAGGA GTCAGCAACT GTCTCAGCCC 601 CTGCACCCAT GAGCCCCACA AGAAGACGAG CCCTGGACCA TTATCTCACC CTTCGAAGCT 661 CTGGATGGAT CCCAGATGGA CGAGGTCGAT GGGTAAAAGA TGAAAATGTT GAGTTTGACT 721 CTGATGAGGA GGAACCACCT GATCTCCCCT TGGACTGCCC AACTTTCTTG TACAAAGTGG 781 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 841 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 901 ATGTATACAG TGCAGACGAG CGACCACGCG TTAAGTCgac aatcaacctc tggattacaa 961 aatttgtgaa agatt