Construct: ORF TRCN0000470478
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001181.1_s317c1
- Derived from:
- ccsbBroadEn_01914
- DNA Barcode:
- ACCAGTGCAGTTCTATCGAGTATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- H4C2 (8366)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470478
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8366 | H4C2 | H4 clustered histone 2 | NM_003544.2 | 100% | 100% | |
| 2 | human | 8359 | H4C1 | H4 clustered histone 1 | NM_003538.3 | 84.7% | 100% | (many diffs) |
| 3 | human | 8294 | H4C9 | H4 clustered histone 9 | NM_003495.2 | 84.4% | 100% | (many diffs) |
| 4 | human | 8362 | H4C12 | H4 clustered histone 12 | NM_003541.2 | 84.1% | 100% | (many diffs) |
| 5 | human | 8367 | H4C5 | H4 clustered histone 5 | NM_003545.3 | 84.1% | 100% | (many diffs) |
| 6 | human | 8363 | H4C11 | H4 clustered histone 11 | NM_021968.3 | 84.1% | 100% | (many diffs) |
| 7 | human | 8364 | H4C3 | H4 clustered histone 3 | NM_003542.3 | 83.8% | 100% | (many diffs) |
| 8 | human | 8361 | H4C6 | H4 clustered histone 6 | NM_003540.3 | 83.4% | 100% | (many diffs) |
| 9 | human | 8365 | H4C8 | H4 clustered histone 8 | NM_003543.3 | 83.4% | 100% | (many diffs) |
| 10 | human | 8368 | H4C13 | H4 clustered histone 13 | NM_003546.2 | 82.2% | 100% | (many diffs) |
| 11 | human | 121504 | H4-16 | H4 histone 16 | NM_175054.2 | 82.2% | 100% | (many diffs) |
| 12 | human | 554313 | H4C15 | H4 clustered histone 15 | NM_001034077.4 | 81.2% | 100% | (many diffs) |
| 13 | human | 8370 | H4C14 | H4 clustered histone 14 | NM_003548.2 | 81.2% | 100% | (many diffs) |
| 14 | human | 8360 | H4C4 | H4 clustered histone 4 | NM_003539.3 | 80.5% | 100% | (many diffs) |
| 15 | mouse | 319158 | Hist1h4i | histone cluster 1, H4i | NM_175656.3 | 84.1% | 100% | (many diffs) |
| 16 | mouse | 100041230 | Hist1h4m | histone cluster 1, H4m | NM_001195421.1 | 83.8% | 100% | (many diffs) |
| 17 | mouse | 69386 | Hist1h4h | histone cluster 1, H4h | NM_153173.4 | 83.8% | 100% | (many diffs) |
| 18 | mouse | 319161 | Hist1h4n | histone cluster 1, H4n | NM_175657.2 | 83.8% | 100% | (many diffs) |
| 19 | mouse | 319156 | Hist1h4d | histone cluster 1, H4d | NM_175654.2 | 83.4% | 100% | (many diffs) |
| 20 | mouse | 326620 | Hist1h4b | histone cluster 1, H4b | NM_178193.2 | 82.8% | 100% | (many diffs) |
| 21 | mouse | 319160 | Hist1h4k | histone cluster 1, H4k | NM_178211.2 | 82.5% | 100% | (many diffs) |
| 22 | mouse | 319155 | Hist1h4c | histone cluster 1, H4c | NM_178208.2 | 82.2% | 100% | (many diffs) |
| 23 | mouse | 319157 | Hist1h4f | histone cluster 1, H4f | NM_175655.2 | 82.2% | 100% | (many diffs) |
| 24 | mouse | 326619 | Hist1h4a | histone cluster 1, H4a | NM_178192.2 | 82.2% | 100% | (many diffs) |
| 25 | mouse | 319159 | Hist1h4j | histone cluster 1, H4j | NM_178210.2 | 81.8% | 100% | (many diffs) |
| 26 | mouse | 320332 | Hist4h4 | histone cluster 4, H4 | NM_175652.3 | 81.2% | 100% | (many diffs) |
| 27 | mouse | 97122 | Hist2h4 | histone cluster 2, H4 | NM_033596.3 | 80.9% | 100% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 399
- ORF length:
- 309
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGTCTGGTCG CGGCAAAGGC GGTAAAGGTT 121 TGGGTAAGGG AGGTGCCAAG CGTCACCGAA AAGTGCTGCG GGATAACATC CAAGGCATCA 181 CCAAACCGGC CATTCGGCGC CTTGCTAGGC GTGGTGGGGT TAAGCGAATT TCCGGTTTGA 241 TTTATGAGGA GACTCGTGGC GTTCTCAAGG TGTTTCTGGA GAACGTGATC CGGGACGCCG 301 TGACCTACAC GGAGCACGCC AAGCGCAAGA CTGTCACTGC CATGGATGTG GTTTACGCGC 361 TCAAGCGTCA AGGACGCACT CTGTACGGCT TCGGCGGTTA CCCAACTTTC TTGTACAAAG 421 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 481 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 541 ACGAACCAGT GCAGTTCTAT CGAGTATTAC GCGTTAAGTC gacaatcaac ctctggatta 601 caaaatttgt gaaagatt