Construct: ORF TRCN0000470515
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009699.1_s317c1
- Derived from:
- ccsbBroadEn_04234
- DNA Barcode:
- TAATGTGCCTTCAAACGGCCTTCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HM13 (81502)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470515
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 81502 | HM13 | histocompatibility minor 13 | NM_030789.3 | 100% | 100% | |
| 2 | human | 81502 | HM13 | histocompatibility minor 13 | NM_178580.2 | 91.2% | 89.8% | (many diffs) |
| 3 | human | 81502 | HM13 | histocompatibility minor 13 | NM_178581.2 | 88.4% | 88.4% | 1035_1181del |
| 4 | human | 81502 | HM13 | histocompatibility minor 13 | NM_178582.3 | 35.3% | 33.3% | (many diffs) |
| 5 | mouse | 14950 | H13 | histocompatibility 13 | NM_010376.4 | 91.4% | 95.7% | (many diffs) |
| 6 | mouse | 14950 | H13 | histocompatibility 13 | NM_001159551.1 | 85.6% | 86.8% | (many diffs) |
| 7 | mouse | 14950 | H13 | histocompatibility 13 | XM_006498783.3 | 80.9% | 84.7% | (many diffs) |
| 8 | mouse | 14950 | H13 | histocompatibility 13 | XR_374416.1 | 61% | (many diffs) | |
| 9 | mouse | 14950 | H13 | histocompatibility 13 | XR_001780858.1 | 54.2% | (many diffs) | |
| 10 | mouse | 14950 | H13 | histocompatibility 13 | XM_017315666.1 | 41% | 26.3% | (many diffs) |
| 11 | mouse | 14950 | H13 | histocompatibility 13 | NM_001159553.1 | 38.8% | 29.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1197
- ORF length:
- 1131
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ctcggccctc agcgatccgc ataacggcag tgccgaggca ggcggcccca 121 ccaacagcac tacgcggccg ccttccacgc ccgagggcat cgcgctggcc tacggcagcc 181 tcctgctcat ggcgctgctg cccatcttct tcggcgccct gcgctccgta cgctgcgccc 241 gcggcaagaa tgcttcagac atgcctgaaa caatcaccag ccgggatgcc gcccgcttcc 301 ccatcatcgc cagctgcaca ctcttggggc tctacctctt tttcaaaata ttctcccagg 361 agtacatcaa cctcctgctg tccatgtatt tcttcgtgct gggaatcctg gccctgtccc 421 acaccatcag ccccttcatg aataagtttt ttccagccag ctttccaaat cgacagtacc 481 agctgctctt cacacagggt tctggggaaa acaaggaaga gatcatcaat tatgaatttg 541 acaccaagga cctggtgtgc ctgggcctga gcagcatcgt tggcgtctgg tacctgctga 601 ggaagcactg gattgccaac aacctttttg gcctggcctt ctcccttaat ggagtagagc 661 tcctgcacct caacaatgtc agcactggct gcatcctgct gggcggactc ttcatctacg 721 atgtcttctg ggtatttggc accaatgtga tggtgacagt ggccaagtcc TTCGAGGCAC 781 CAATAAAATT GGTGTTTCCC CAGGATCTGC TGGAGAAAGG CCTCGAAGCA AACAACTTTG 841 CCATGCTGGG ACTTGGAGAT GTCGTCATTC CAGGGATCTT CATTGCCTTG CTGCTGCGCT 901 TTGACATCAG CTTGAAGAAG AATACCCACA CCTACTTCTA CACCAGCTTT GCAGCCTACA 961 TCTTCGGCCT GGGCCTTACC ATCTTCATCA TGCACATCTT CAAGCATGCT CAGCCTGCCC 1021 TCCTATACCT GGTCCCCGCC TGCATCGGTT TTCCTGTCCT GGTGGCGCTG GCCAAGGGAG 1081 AAGTGACAGA GATGTTCAGT TATGAGGAGT CAAATCCTAA GGATCCAGCG GCAGTGACAG 1141 AATCCAAAGA GGGAACAGAG GCATCAGCAT CGAAGGGGCT GGAGAAGAAA GAGAAATGCT 1201 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1261 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1321 ATATATCTTG TGGAAAGGAC GATAATGTGC CTTCAAACGG CCTTCCACGC GTTAAGTCga 1381 caatcaacct ctggattaca aaatttgtga aagatt