Construct: ORF TRCN0000470515

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009699.1_s317c1
Derived from:
ccsbBroadEn_04234
DNA Barcode:
TAATGTGCCTTCAAACGGCCTTCC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
HM13 (81502)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000470515
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 81502 HM13 histocompatibility minor 13 NM_030789.3 100% 100%
2 human 81502 HM13 histocompatibility minor 13 NM_178580.2 91.2% 89.8% (many diffs)
3 human 81502 HM13 histocompatibility minor 13 NM_178581.2 88.4% 88.4% 1035_1181del
4 human 81502 HM13 histocompatibility minor 13 NM_178582.3 35.3% 33.3% (many diffs)
5 mouse 14950 H13 histocompatibility 13 NM_010376.4 91.4% 95.7% (many diffs)
6 mouse 14950 H13 histocompatibility 13 NM_001159551.1 85.6% 86.8% (many diffs)
7 mouse 14950 H13 histocompatibility 13 XM_006498783.3 80.9% 84.7% (many diffs)
8 mouse 14950 H13 histocompatibility 13 XR_374416.1 61% (many diffs)
9 mouse 14950 H13 histocompatibility 13 XR_001780858.1 54.2% (many diffs)
10 mouse 14950 H13 histocompatibility 13 XM_017315666.1 41% 26.3% (many diffs)
11 mouse 14950 H13 histocompatibility 13 NM_001159553.1 38.8% 29.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1197
ORF length:
1131
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgga ctcggccctc agcgatccgc ataacggcag tgccgaggca ggcggcccca
121ccaacagcac tacgcggccg ccttccacgc ccgagggcat cgcgctggcc tacggcagcc
181tcctgctcat ggcgctgctg cccatcttct tcggcgccct gcgctccgta cgctgcgccc
241gcggcaagaa tgcttcagac atgcctgaaa caatcaccag ccgggatgcc gcccgcttcc
301ccatcatcgc cagctgcaca ctcttggggc tctacctctt tttcaaaata ttctcccagg
361agtacatcaa cctcctgctg tccatgtatt tcttcgtgct gggaatcctg gccctgtccc
421acaccatcag ccccttcatg aataagtttt ttccagccag ctttccaaat cgacagtacc
481agctgctctt cacacagggt tctggggaaa acaaggaaga gatcatcaat tatgaatttg
541acaccaagga cctggtgtgc ctgggcctga gcagcatcgt tggcgtctgg tacctgctga
601ggaagcactg gattgccaac aacctttttg gcctggcctt ctcccttaat ggagtagagc
661tcctgcacct caacaatgtc agcactggct gcatcctgct gggcggactc ttcatctacg
721atgtcttctg ggtatttggc accaatgtga tggtgacagt ggccaagtcc TTCGAGGCAC
781CAATAAAATT GGTGTTTCCC CAGGATCTGC TGGAGAAAGG CCTCGAAGCA AACAACTTTG
841CCATGCTGGG ACTTGGAGAT GTCGTCATTC CAGGGATCTT CATTGCCTTG CTGCTGCGCT
901TTGACATCAG CTTGAAGAAG AATACCCACA CCTACTTCTA CACCAGCTTT GCAGCCTACA
961TCTTCGGCCT GGGCCTTACC ATCTTCATCA TGCACATCTT CAAGCATGCT CAGCCTGCCC
1021TCCTATACCT GGTCCCCGCC TGCATCGGTT TTCCTGTCCT GGTGGCGCTG GCCAAGGGAG
1081AAGTGACAGA GATGTTCAGT TATGAGGAGT CAAATCCTAA GGATCCAGCG GCAGTGACAG
1141AATCCAAAGA GGGAACAGAG GCATCAGCAT CGAAGGGGCT GGAGAAGAAA GAGAAATGCT
1201CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC
1261TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT
1321ATATATCTTG TGGAAAGGAC GATAATGTGC CTTCAAACGG CCTTCCACGC GTTAAGTCga
1381caatcaacct ctggattaca aaatttgtga aagatt

Download FASTA (ORF) (Full)