Transcript: Human NM_178581.2

Homo sapiens histocompatibility minor 13 (HM13), transcript variant 3, mRNA.

Source:
NCBI, updated 2019-09-21
Taxon:
Homo sapiens (human)
Gene:
HM13 (81502)
Length:
1771
CDS:
143..1423

Additional Resources:

NCBI RefSeq record:
NM_178581.2
NBCI Gene record:
HM13 (81502)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_178581.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000073751 CGGACTCTTCATCTACGATGT pLKO.1 781 CDS 100% 4.050 5.670 N HM13 n/a
2 TRCN0000289305 CGGACTCTTCATCTACGATGT pLKO_005 781 CDS 100% 4.050 5.670 N HM13 n/a
3 TRCN0000310266 GTCCTTCGAGGCACCAATAAA pLKO_005 844 CDS 100% 15.000 12.000 N HM13 n/a
4 TRCN0000073749 GCGCTTTGACATCAGCTTGAA pLKO.1 973 CDS 100% 4.950 3.960 N HM13 n/a
5 TRCN0000289303 GCGCTTTGACATCAGCTTGAA pLKO_005 973 CDS 100% 4.950 3.960 N HM13 n/a
6 TRCN0000073748 CCTCTGTTTCCTTCTCCCTTT pLKO.1 1573 3UTR 100% 4.050 2.835 N HM13 n/a
7 TRCN0000296186 TCAGGGCCAGACCAGACAGAT pLKO_005 1445 3UTR 100% 1.650 1.155 N HM13 n/a
8 TRCN0000073752 GCTTCAGACATGCCTGAAACA pLKO.1 329 CDS 100% 0.495 0.347 N HM13 n/a
9 TRCN0000289304 GCTTCAGACATGCCTGAAACA pLKO_005 329 CDS 100% 0.495 0.347 N HM13 n/a
10 TRCN0000073750 CGAAGCAAACAACTTTGCCAT pLKO.1 901 CDS 100% 0.264 0.185 N HM13 n/a
11 TRCN0000030529 CCAGGAGTACATCAACCTCTT pLKO.1 433 CDS 100% 4.050 2.835 N H13 n/a
12 TRCN0000297555 CCAGGAGTACATCAACCTCTT pLKO_005 433 CDS 100% 4.050 2.835 N H13 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_178581.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04234 pDONR223 100% 88.4% 88.4% None 1035_1181del n/a
2 ccsbBroad304_04234 pLX_304 0% 88.4% 88.4% V5 1035_1181del n/a
3 TRCN0000470515 TAATGTGCCTTCAAACGGCCTTCC pLX_317 37.9% 88.4% 88.4% V5 1035_1181del n/a
4 TRCN0000488993 TTATAGCGGGGCCCCGTCGCTGGC pLX_317 30.2% 88.4% 88.2% V5 (not translated due to prior stop codon) 704C>T;1035_1181del n/a
5 TRCN0000492118 TAGAATGGAGCGGACGGCAGTAGG pLX_317 31.7% 88.3% 88% V5 704C>T;1035_1181del;1278_1279insG n/a
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