Construct: ORF TRCN0000470560
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000173.1_s317c1
- Derived from:
- ccsbBroadEn_04066
- DNA Barcode:
- ATGATTTACGCTCCTGAGTCAACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CENPO (79172)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470560
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79172 | CENPO | centromere protein O | NM_001322101.2 | 100% | 100% | |
| 2 | human | 79172 | CENPO | centromere protein O | NM_024322.3 | 100% | 100% | |
| 3 | human | 79172 | CENPO | centromere protein O | NM_001199803.3 | 94.1% | 93.4% | 0_1ins28;6_7insAGTCCAAA;10_27del |
| 4 | human | 79172 | CENPO | centromere protein O | XR_244957.2 | 67.4% | 1_231del;560_561ins101;994_995ins36 | |
| 5 | human | 79172 | CENPO | centromere protein O | NR_136183.2 | 22.1% | (many diffs) | |
| 6 | human | 79172 | CENPO | centromere protein O | NR_136182.2 | 19.5% | (many diffs) | |
| 7 | human | 79172 | CENPO | centromere protein O | NR_136184.2 | 19.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 966
- ORF length:
- 900
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gcaggcgaac cctttacgtc cagatggcga gtccaaagga ggtgttttag 121 ctcacttgga aaggctagag acccaagtga gcagatcccg taaacagtct gaagagctgc 181 agagcgtgca ggcccaggaa ggtgctcttg gaaccaagat tcataaacta aggcgtctgc 241 gagatgagct gagggctgtg gtgcggcacc ggcgagccag cgtgaaagca tgtattgcca 301 atgtagaacc caaccaaaca gtggagatca atgagcaaga agcattggaa gagaaattgg 361 aaaatgtgaa agccattctg caggcatatc attttacagg cctcagtggt aaactgacca 421 gccgaggagt ttgtgtctgc atcagtactg cttttgaggg gaacctattg gattcctatt 481 ttgtggacct tgtcatacag aaaccactcc ggatacatca ccattcagtc ccagtcttca 541 ttcCCCTGGA AGAGATAGCT GCAAAATATT TACAGACCAA CATCCAGCAC TTCCTGTTCA 601 GTCTCTGCGA GTACCTGAAT GCTTACTCTG GGAGGAAGTA CCAGGCAGAC CGGCTTCAGA 661 GTGACTTTGC AGCCCTCCTG ACTGGGCCCT TGCAGAGAAA CCCACTGTGT AACTTGCTGT 721 CATTTACTTA CAAACTGGAT CCAGGGGGTC AGTCCTTCCC GTTCTGTGCT AGATTGCTGT 781 ATAAGGACCT CACAGCAACT CTTCCCACTG ACGTCACCGT GACATGTCAA GGAGTGGAAG 841 TATTATCCAC TTCATGGGAG GAGCAACGAG CATCTCATGA AACTCTGTTC TGTACGAAGC 901 CCTTGCATCA AGTGTTTGCC TCATTTACAA GAAAAGGAGA AAAGTTGGAT ATGAGTCTGG 961 TCTCCTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1021 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1081 CTTGGCTTTA TATATCTTGT GGAAAGGACG AATGATTTAC GCTCCTGAGT CAACAACGCG 1141 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt