Construct: ORF TRCN0000470593
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015149.1_s317c1
- Derived from:
- ccsbBroadEn_09328
- DNA Barcode:
- TAGGAACGATCGATGTACGAGGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC25A51 (92014)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470593
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 92014 | SLC25A51 | solute carrier family 25 me... | NM_033412.4 | 99.8% | 100% | 880T>C |
| 2 | human | 147407 | SLC25A52 | solute carrier family 25 me... | NM_001034172.4 | 97.5% | 95.9% | (many diffs) |
| 3 | human | 442229 | SLC25A51P1 | SLC25A51 pseudogene 1 | NR_026540.1 | 51.7% | (many diffs) | |
| 4 | human | 494141 | SLC25A51P4 | SLC25A51 pseudogene 4 | NR_026564.1 | 46% | (many diffs) | |
| 5 | human | 494141 | SLC25A51P4 | SLC25A51 pseudogene 4 | NR_026563.1 | 42.5% | (many diffs) | |
| 6 | human | 494141 | SLC25A51P4 | SLC25A51 pseudogene 4 | NR_026541.1 | 38.3% | (many diffs) | |
| 7 | mouse | 230125 | Slc25a51 | solute carrier family 25, m... | NM_001009949.3 | 80.5% | 86.5% | (many diffs) |
| 8 | mouse | 230125 | Slc25a51 | solute carrier family 25, m... | XM_006537835.3 | 80.5% | 86.5% | (many diffs) |
| 9 | mouse | 230125 | Slc25a51 | solute carrier family 25, m... | XM_017320126.1 | 76.2% | 81.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 957
- ORF length:
- 891
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat ggattcagaa gctcatgaaa agaggccacc aatactaaca tcttcaaaac 121 aagatatatc acctcatatt acaaatgttg gtgagatgaa gcattacttg tgtggctgct 181 gtgcagcctt caacaatgtc gcaatcacat ttcccattca gaaggtcctc tttcgacaac 241 agctgtatgg catcaaaacc cgggatgcaa tacttcagtt gagaagggat ggatttcgaa 301 atttgtatcg tggaatcctt cccccattga tgcagaagac aactacgctt gcacttatgt 361 ttggtctgta tgaggattta tcctgccttc tccacaagca tgtcagtgct ccagagtttg 421 caaccagtgg cgtggcggca gtgcttgcag ggacaacaga agcaattttc actccactgg 481 aaagagttca gacattgctt caagaccaca agcatcatga caaatttacc aacacttacc 541 aggctttcaa ggcactgaaa tgtcatggaa ttggagagta ttatcgaggc ttggtgccca 601 ttcttttccg gaatggacTC AGCAATGTCT TGTTTTTCGG CCTTCGAGGT CCCATTAAGG 661 AGCATCTGCC TACCGCAACG ACTCACAGTG CTCATCTGGT CAATGATTTT ATCTGTGGAG 721 GTCTATTGGG TGCCATGTTG GGATTCTTGT TTTTTCCAAT TAATGTTGTA AAAACTCGCA 781 TACAGTCTCA GATTGGTGGG GAATTTCAGT CTTTCCCCAA GGTTTTCCAA AAAATCTGGC 841 TGGAACGGGA CAGAAAACTG ATAAATCTTT TCAGAGGTGC CCATCTGAAT TACCATCGGT 901 CCCTCATCTC TTGGGGCATA ATCAATGCAA CTTATGAGTT CTTGCTAAAG GTTATATGCC 961 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1021 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1081 ATATATCTTG TGGAAAGGAC GATAGGAACG ATCGATGTAC GAGGCAACGC GTTAAGTCga 1141 caatcaacct ctggattaca aaatttgtga aagatt