Construct: ORF TRCN0000470622
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009183.1_s317c1
- Derived from:
- ccsbBroadEn_03644
- DNA Barcode:
- CCATGCCGACCTGTAGCTGACCCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ARFGAP1 (55738)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470622
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55738 | ARFGAP1 | ADP ribosylation factor GTP... | NM_175609.3 | 100% | 100% | |
| 2 | human | 55738 | ARFGAP1 | ADP ribosylation factor GTP... | XM_011528901.2 | 99.5% | 99.5% | 863_868delAGGTCC |
| 3 | human | 55738 | ARFGAP1 | ADP ribosylation factor GTP... | XM_017027950.1 | 99.5% | 99.5% | 863_868delAGGTCC |
| 4 | human | 55738 | ARFGAP1 | ADP ribosylation factor GTP... | XM_006723823.3 | 97.5% | 97.5% | 716_717ins30 |
| 5 | human | 55738 | ARFGAP1 | ADP ribosylation factor GTP... | NM_018209.4 | 97.1% | 97.1% | 716_717ins30;833_838delAGGTCC |
| 6 | human | 55738 | ARFGAP1 | ADP ribosylation factor GTP... | NM_001281483.1 | 86.1% | 86.2% | 1_1delAins166;3G>C;6_11delAACAAC |
| 7 | human | 55738 | ARFGAP1 | ADP ribosylation factor GTP... | NM_001281484.1 | 69.9% | 63.4% | (many diffs) |
| 8 | human | 55738 | ARFGAP1 | ADP ribosylation factor GTP... | NM_001281482.2 | 64.8% | 65.1% | 716_717ins30;833_1077del;1209_1210ins248 |
| 9 | human | 55738 | ARFGAP1 | ADP ribosylation factor GTP... | XR_001754329.2 | 40% | 1_112del;975_980delAGGTCC;1361_3099del | |
| 10 | human | 55738 | ARFGAP1 | ADP ribosylation factor GTP... | XR_001754330.2 | 35.6% | 1_112del;797_1007del;1566_3485del | |
| 11 | human | 55738 | ARFGAP1 | ADP ribosylation factor GTP... | XR_936575.2 | 35.5% | (many diffs) | |
| 12 | human | 55738 | ARFGAP1 | ADP ribosylation factor GTP... | XR_936576.2 | 34.7% | (many diffs) | |
| 13 | human | 55738 | ARFGAP1 | ADP ribosylation factor GTP... | XR_936574.2 | 31.7% | (many diffs) | |
| 14 | human | 55738 | ARFGAP1 | ADP ribosylation factor GTP... | NR_104022.2 | 20.3% | (many diffs) | |
| 15 | mouse | 228998 | Arfgap1 | ADP-ribosylation factor GTP... | XM_006500594.3 | 83.4% | 84.9% | (many diffs) |
| 16 | mouse | 228998 | Arfgap1 | ADP-ribosylation factor GTP... | XM_011239935.1 | 83.4% | 84.9% | (many diffs) |
| 17 | mouse | 228998 | Arfgap1 | ADP-ribosylation factor GTP... | NM_145760.3 | 81.3% | 83% | (many diffs) |
| 18 | mouse | 228998 | Arfgap1 | ADP-ribosylation factor GTP... | XM_017318014.1 | 81.3% | 83% | (many diffs) |
| 19 | mouse | 228998 | Arfgap1 | ADP-ribosylation factor GTP... | NM_001177707.1 | 77.6% | 79.1% | (many diffs) |
| 20 | mouse | 228998 | Arfgap1 | ADP-ribosylation factor GTP... | NM_001177708.1 | 77.6% | 79.1% | (many diffs) |
| 21 | mouse | 228998 | Arfgap1 | ADP-ribosylation factor GTP... | NM_001177706.1 | 77.2% | 78.9% | (many diffs) |
| 22 | mouse | 228998 | Arfgap1 | ADP-ribosylation factor GTP... | NM_001177709.1 | 77.2% | 78.9% | (many diffs) |
| 23 | mouse | 228998 | Arfgap1 | ADP-ribosylation factor GTP... | NM_001177710.1 | 69.2% | 69.8% | (many diffs) |
| 24 | mouse | 228998 | Arfgap1 | ADP-ribosylation factor GTP... | XM_011239936.1 | 57.8% | 52.5% | (many diffs) |
| 25 | mouse | 228998 | Arfgap1 | ADP-ribosylation factor GTP... | XR_001782401.1 | 40% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1308
- ORF length:
- 1242
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cagcccaaga accaggaagg ttcttaaaga agtcagggtg caggatgaga 121 acaacgtttg ttttgagtgt ggcgcgttca atcctcagtg ggtcagtgtg acctacggca 181 tctggatctg cctggagtgc tcggggagac accgcgggct tggggttcac ctcagctttg 241 tgcgctctgt tactatggac aagtggaagg acattgagct tgagaagatg aaagctggtg 301 ggaatgctaa gttccgagag ttcctggagt ctcaggagga ttacgatcct tgctggtcct 361 tgcaggagaa gtacaacagc agagccgcgg ccctctttag ggataaggtg gtcgctctgg 421 ccgaaggcag agagtggtct ctggagtcat cacctgccca gaactggacc ccacctcagc 481 ccaggacgct gccgtccatg gtgcaccgag tctctggcca gccgcagagt gtgaccgcct 541 cctcggacaa ggcttttgaa gactggctga atgatgacct cggctcctat caaggggccc 601 aggggaatcg ctacgtgggg tttgggaaca cgccaccgcc tcagaagaaa gaagatgact 661 tcctcaacaa cgccatgtcc tccctgtact cgggctggag cagcttcacc actggagcca 721 gccggtttgc ctcggcagcc aaggagggcg ctacaaagtt tggatcccaa gcgagtcaga 781 agttttgggg tcacaagcag cagccggagc cggcgtccga gctgggccac agcctgaacg 841 agaacgtcct caagcctgcg caggagaagg tgaaggaggg aaagattttt GATGATGTCT 901 CCAGTGGGGT CTCTCAGTTG GCGTCCAAGG GAGTCGGTAG TAAGGGATGG CGGGACGTCA 961 CCACCTTTTT TTCGGGGAAA GCAGAGGGCC CCTTGGACAG CCCCTCGGAG GGCCACAGTT 1021 ATCAGAACAG CGGTCTGGAC CACTTCCAAA ACAGCAACAT AGACCAGAGC TTCTGGGAGA 1081 CCTTTGGAAG TGCTGAGCCC ACCAAGACCC GCAAGTCCCC GAGCAGCGAC AGCTGGACGT 1141 GCGCGGACAC CTCCACCGAG AGGAGGAGCT CGGACAGCTG GGAGGTGTGG GGCTCGGCCT 1201 CCACCAACAG GAACAGCAAC AGCGACGGCG GGGAGGGCGG GGAGGGCACC AAGAAGGCAG 1261 TGCCGCCGGC CGTGCCCACT GATGATGGCT GGGACAACCA GAACTGGTAC CCAACTTTCT 1321 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1381 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1441 GTGGAAAGGA CGACCATGCC GACCTGTAGC TGACCCTACG CGTTAAGTCg acaatcaacc 1501 tctggattac aaaatttgtg aaagatt