Construct: ORF TRCN0000470647
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014661.1_s317c1
- Derived from:
- ccsbBroadEn_09274
- DNA Barcode:
- TTCCCAGCTACGACTCCCCGATTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CHRFAM7A (89832)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470647
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 89832 | CHRFAM7A | CHRNA7 (exons 5-10) and FAM... | NM_139320.1 | 99.6% | 100% | (many diffs) |
| 2 | human | 89832 | CHRFAM7A | CHRNA7 (exons 5-10) and FAM... | XM_011522153.2 | 99.6% | 100% | (many diffs) |
| 3 | human | 1139 | CHRNA7 | cholinergic receptor nicoti... | XM_011521177.2 | 95.6% | 93.9% | (many diffs) |
| 4 | human | 1139 | CHRNA7 | cholinergic receptor nicoti... | XM_017021883.2 | 89.2% | 84.8% | (many diffs) |
| 5 | human | 1139 | CHRNA7 | cholinergic receptor nicoti... | XM_017021882.1 | 89% | 84.6% | (many diffs) |
| 6 | human | 1139 | CHRNA7 | cholinergic receptor nicoti... | XM_011521176.3 | 82.7% | 79.4% | (many diffs) |
| 7 | human | 1139 | CHRNA7 | cholinergic receptor nicoti... | NM_000746.6 | 80% | 78% | (many diffs) |
| 8 | human | 1139 | CHRNA7 | cholinergic receptor nicoti... | XM_017021884.1 | 77.6% | 77.9% | (many diffs) |
| 9 | human | 89832 | CHRFAM7A | CHRNA7 (exons 5-10) and FAM... | NM_148911.1 | 77.5% | 77.9% | (many diffs) |
| 10 | human | 89832 | CHRFAM7A | CHRNA7 (exons 5-10) and FAM... | XM_005254750.3 | 77.5% | 77.9% | (many diffs) |
| 11 | human | 1139 | CHRNA7 | cholinergic receptor nicoti... | NM_001190455.3 | 75.7% | 73.8% | (many diffs) |
| 12 | human | 89832 | CHRFAM7A | CHRNA7 (exons 5-10) and FAM... | XM_011522155.3 | 70.1% | 55.5% | (many diffs) |
| 13 | human | 1139 | CHRNA7 | cholinergic receptor nicoti... | XM_011521178.3 | 55.9% | 41.7% | (many diffs) |
| 14 | human | 1139 | CHRNA7 | cholinergic receptor nicoti... | NR_046324.1 | 36.4% | (many diffs) | |
| 15 | mouse | 11441 | Chrna7 | cholinergic receptor, nicot... | NM_007390.3 | 70.2% | 72.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1305
- ORF length:
- 1236
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcaaaaatat tgcatctacc agcattttca gttccaattg ctaatccagc 121 atttgtggat agctgcaaac tgcgatattg ctgatgagcg ctttgacgcc acattccaca 181 ctaacgtgtt ggtgaattct tctgggcatt gccagtacct gcctccaggc atattcaaga 241 gttcctgcta catcgatgta cgctggtttc cctttgatgt gcagcactgc aaactgaagt 301 ttgggtcctg gtcttacgga ggctggtcct tggatctgca gatgcaggag gcagatatca 361 gtggctatat ccccaatgga gaatgggacc tagtgggaat ccccggcaag aggagtgaaa 421 ggttctatga gtgctgcaaa gagccctacc ccgatgtcac cttcacagtg accatgcgcc 481 gcaggacact ctactatggc ctcaacctgc tgatcccctg tgtgctcatc tccgccctcg 541 ccctgctggt gttcctgctt cctgcagatt ccggggagaa gatttccctg gggataacag 601 tcttactctc tcttaccgtc ttcatgctgc tcgtggctga gatcatgccc gcaacatccg 661 attcggtacc attgatagcc cagtacttcg ccagcaccat gatcatcgtg ggcctctcgg 721 tggtggtgac ggtgatcgtg ctgcagtacc accaccacga ccccgacggg ggcaagatgc 781 ccaagtggac cagagtcatc cttctgaact ggtgcgcgtg gttcctgcga atGAAGAGGC 841 CCGGGGAGGA CAAGGTGCGC CCGGCCTGCC AGCACAAGCA GCGGCGCTGC AGCCTGGCCA 901 GTGTGGAGAT GAGCGCCGTG GCGCCGCCGC CCGCCAGCAA CGGGAACCTG CTGTACATCG 961 GCTTCCGCGG CCTGGACGGC GTGCACTGTG TCCCGACCCC CGACTCTGGG GTAGTGTGTG 1021 GCCGCATGGC CTGCTCCCCC ACGCACGATG AGCACCTCCT GCACGGTGGG CAACCCCCCG 1081 AGGGGGACCC GGACTTGGCC AAGATCCTGG AGGAGGTCCG CTACATTGCC AACCGCTTCC 1141 GCTGCCAGGA CGAAAGCGAG GCGGTCTGCA GCGAGTGGAA GTTCGCCGCC TGTGTGGTGG 1201 ACCGCCTGTG CCTCATGGCC TTCTCGGTCT TCACCATCAT CTGCACCATC GGCATCCTGA 1261 TGTCGGCTCC CAACTTCGTG GAGGCCGTGT CCAAAGACTT TGCGTTGCCA ACTTTCTTGT 1321 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1381 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1441 GAAAGGACGA TTCCCAGCTA CGACTCCCCG ATTCACGCGT TAAGTCgaca atcaacctct 1501 ggattacaaa atttgtgaaa gatt