Construct: ORF TRCN0000470659
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007272.1_s317c1
- Derived from:
- ccsbBroadEn_14082
- DNA Barcode:
- CGTTAAGTTTAGGACCCAGTAGAC
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ECPAS (23392)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470659
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23392 | ECPAS | Ecm29 proteasome adaptor an... | XM_017014557.1 | 36.4% | 36% | 1_2709del;4245_4246insAA |
| 2 | human | 23392 | ECPAS | Ecm29 proteasome adaptor an... | NM_001363756.1 | 28.2% | 27.9% | 1_3960del;5496_5497insAA |
| 3 | human | 23392 | ECPAS | Ecm29 proteasome adaptor an... | NM_001364929.1 | 28.2% | 27.9% | 1_3960del;5496_5497insAA |
| 4 | human | 23392 | ECPAS | Ecm29 proteasome adaptor an... | NM_001364930.1 | 28.2% | 27.9% | 1_3960del;5496_5497insAA |
| 5 | human | 23392 | ECPAS | Ecm29 proteasome adaptor an... | XM_005251853.3 | 28.2% | 27.9% | 1_3960del;5496_5497insAA |
| 6 | human | 23392 | ECPAS | Ecm29 proteasome adaptor an... | NM_001364931.1 | 28.1% | 27.8% | 1_3978del;5514_5515insAA |
| 7 | human | 23392 | ECPAS | Ecm29 proteasome adaptor an... | XM_011518422.3 | 25.7% | 25.6% | (many diffs) |
| 8 | mouse | 230249 | AI314180 | expressed sequence AI314180 | XM_011250007.2 | 27.6% | 28.9% | (many diffs) |
| 9 | mouse | 230249 | AI314180 | expressed sequence AI314180 | XM_006537850.3 | 25.8% | 27% | (many diffs) |
| 10 | mouse | 230249 | AI314180 | expressed sequence AI314180 | NM_172381.2 | 25.1% | 26.3% | (many diffs) |
| 11 | mouse | 230249 | AI314180 | expressed sequence AI314180 | XM_006537848.3 | 25.1% | 26.3% | (many diffs) |
| 12 | mouse | 230249 | AI314180 | expressed sequence AI314180 | XM_006537849.3 | 25.1% | 26.3% | (many diffs) |
| 13 | mouse | 230249 | AI314180 | expressed sequence AI314180 | XM_006537847.3 | 25% | 26.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1626
- ORF length:
- 1560
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga tagtgctcgg cttagtgctg ccaaatcttc tccaatgatg gaaacaatca 121 acatgtgcct gcaatacctt gatgtgtcag tgctgggcga gctagttcct aggttgtgtg 181 aactgatcag aagtggtgta ggtcttggaa ctaagggtgg ctgtgccagt gtcattgtgt 241 cattaactac tcagtgtcct caggacctaa caccttactc aggtaaactt atgagtgctt 301 tgctgagtgg cctgacagat cggaacagtg tgattcagaa atcttgtgca tttgctatgg 361 gccatttagt tcggacctca cgggatagca gcactgaaaa actcctgcag aagctcaatg 421 ggtggtatat ggagaaagaa gaacctatct acaagacctc ttgtgctttg actattcatg 481 ctattggacg atacagccct gatgtattaa agaatcatgc aaaagaagtc ctgcctctgg 541 catttttagg catgcatgaa attgctgatg aggagaaatc cgaaaaagaa gaatgtaatt 601 tatggaccga agtgtggcag gaaaacgtac ctggatcctt tggtggcatt cgattatacc 661 tgcaggagtt aattactatt acccagaagg ctttgcagtc tcagtcctgg aaaatgaaag 721 cccagggtgc aattgccatg gcatcaattg caaaacagac tagctctcta gtacctccat 781 atctcggaat gatactgacc gcattgctgc aaggcctggc tggaagaacg tgggcaggaa 841 aggaggagct attgaaagcc attgcctgtg tggtgacagc ttgcagtgca gagctggaaa 901 agtctgtgcc caatcaaccc agcacaaatg aaattcttca agctgttctg aaggaatgta 961 gcaaagagaa tgtcaaatac aagattgtag caatcagctg tgcagctgat atcttgaagg 1021 ccaccaaaga ggacagattc caggagttct ctaacattgt catacctctc atcaagaaga 1081 actcacttga aagcagtggg gtccggacaa ccaaaaatga agaggagaat gaaaaggaaa 1141 aggagctcca gctggaatat ctgctgggtg cctttGAAAG CCTGGGCAAA GCCTGGCCGC 1201 GAAACGCGGA GACCCAACGT TGTTATCGTC AGGAGCTGTG CAAACTGATG TGTGAACGGC 1261 TAAAACTCAG CACGTGGAAA GTGCAGCTAG GAGTCCTGCA ATCAATGAAT GCCTTTTTTC 1321 AGGGGTTAAT GCTTTTGGAA GAAGAACATG CCGATCCTGA GGCTTTGGCT GAAATTCTGC 1381 TTGAAACTTG TAAATCAATC ACATATTCTT TAGAAAATAA GACCTACTCA TCTGTGAGAA 1441 CAGAAGCTTT ATCTGTGATA GAATTGCTGC TTAAAAAACT TGAAGAATCT AAACAGTGGG 1501 AATGTTTGAC ATCTGAATGC AGAGTGCTCC TAATTGAGTC TTTAGCTACT ATGGAGCCAG 1561 ACAGCAGACC TGAACTGCAG GAGAAAGCAG CGTTACTGAA GAAAAAACAC TTGAAAATCT 1621 GGAATACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT 1681 CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC 1741 TTGGCTTTAT ATATCTTGTG GAAAGGACGA CGTTAAGTTT AGGACCCAGT AGACACGCGT 1801 TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt