Construct: ORF TRCN0000470694

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018322.1_s317c1
Derived from:
ccsbBroadEn_02913
DNA Barcode:
GCTTGAAGCTGGCGCGCCCGTGAT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
HERC4 (26091)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000470694
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 26091 HERC4 HECT and RLD domain contain... NM_015601.4 100% 100%
2 human 26091 HERC4 HECT and RLD domain contain... XM_024447931.1 100% 100%
3 human 26091 HERC4 HECT and RLD domain contain... NM_022079.3 99.2% 99.2% 1926_1949del
4 human 26091 HERC4 HECT and RLD domain contain... XM_024447929.1 99.2% 99.2% 1926_1949del
5 human 26091 HERC4 HECT and RLD domain contain... XM_024447930.1 99.2% 99.2% 1926_1949del
6 human 26091 HERC4 HECT and RLD domain contain... XM_011539593.3 97.7% 97.7% 384_455del
7 human 26091 HERC4 HECT and RLD domain contain... XM_011539592.3 97% 97% 384_455del;1998_2021del
8 human 26091 HERC4 HECT and RLD domain contain... XM_024447927.1 97% 97% 384_455del;1998_2021del
9 human 26091 HERC4 HECT and RLD domain contain... XM_024447928.1 97% 97% 384_455del;1998_2021del
10 human 26091 HERC4 HECT and RLD domain contain... XM_011539596.2 92.5% 92.1% (many diffs)
11 human 26091 HERC4 HECT and RLD domain contain... NM_001278185.2 91.8% 91.8% 1926_1949del;2360_2361ins234
12 human 26091 HERC4 HECT and RLD domain contain... XM_011539594.2 90.5% 90.1% (many diffs)
13 human 26091 HERC4 HECT and RLD domain contain... XM_011539595.2 90.5% 90.1% (many diffs)
14 human 26091 HERC4 HECT and RLD domain contain... XM_017016041.2 87.7% 87.7% 1442_1443ins363;1563_1586del
15 human 26091 HERC4 HECT and RLD domain contain... XM_011539597.3 85.8% 85.8% 384_455del;1514_1515ins363;1635_1658del
16 human 26091 HERC4 HECT and RLD domain contain... NM_001278186.2 75.5% 75.3% (many diffs)
17 human 26091 HERC4 HECT and RLD domain contain... XR_001747081.2 53.3% (many diffs)
18 human 26091 HERC4 HECT and RLD domain contain... XM_024447932.1 50.5% 50.5% 0_1ins1545;381_404del
19 human 26091 HERC4 HECT and RLD domain contain... NM_001278187.2 9.3% 7.5% (many diffs)
20 human 26091 HERC4 HECT and RLD domain contain... XM_024447933.1 9.3% 7.5% (many diffs)
21 mouse 67345 Herc4 hect domain and RLD 4 NM_026101.4 91.6% 96.9% (many diffs)
22 mouse 67345 Herc4 hect domain and RLD 4 NM_030114.2 90.9% 96.2% (many diffs)
23 mouse 67345 Herc4 hect domain and RLD 4 XM_006513985.1 84.9% 89.4% (many diffs)
24 mouse 67345 Herc4 hect domain and RLD 4 XM_006513987.2 80.6% 85.5% (many diffs)
25 mouse 67345 Herc4 hect domain and RLD 4 XM_006513986.3 80% 84.8% (many diffs)
26 mouse 67345 Herc4 hect domain and RLD 4 XM_006513988.3 77.8% 80.9% (many diffs)
27 mouse 67345 Herc4 hect domain and RLD 4 XR_380431.3 72.1% (many diffs)
28 mouse 67345 Herc4 hect domain and RLD 4 XM_006513990.3 69.1% 73.1% (many diffs)
29 mouse 67345 Herc4 hect domain and RLD 4 XM_006513989.3 68.6% 72.5% (many diffs)
30 mouse 67345 Herc4 hect domain and RLD 4 XR_380432.3 50.3% (many diffs)
31 mouse 67345 Herc4 hect domain and RLD 4 XM_006513991.3 47.5% 49.7% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
3213
ORF length:
3147
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgtt gtgctgggga aatgcatcct ttgggcagct aggtttgggt ggaattgatg
121aagaaattgt actagagccc agaaaaagtg acttctttat aaataaaagg gtccgagatg
181taggatgtgg actcagacat actgtgtttg ttctggatga tggaacagtg tacacatgtg
241gatgtaatga tctaggacag ctaggtcatg aaaaatccag aaagaaacca gagcaggttg
301ttgccctgga tgcccaaaat attgtagctg tttcatgtgg agaagctcat acgttagcgc
361taaatgacaa aggccaggtg tatgcttggg gtctcgattc tgatggacag cttggcctgg
421taggatcaga ggaatgcatc agagtaccca gaaatattaa aagtttgtca gatatccaga
481ttgtacaggt tgcttgtggt tactatcatt cacttgcact ttctaaagca agtgaagtct
541tctgttgggg acagaataaa tatggccaat tgggtttagg tactgactgt aaaaagcaaa
601cttcaccgca gctgcttaag tctttgcttg gaatcccttt catgcaagtt gcagcaggag
661gagcccatag ttttgtactc accctttctg gagctatctt tggatgggga cgcaacaagt
721ttggtcagct aggtcttaat gatgaaaatg ataggtatgt tcctaattta ctaaagtcac
781taagatctca gaaaatagtt tatatttgtt gtggagaaga tcatactgct gctctaacca
841aggaaggtgg agtgtttact tttggagctg gagggtatgg tcagttgggc cataattcta
901ccagtcatga aataaaccca aggaaagttt ttgaacttat gggaagcatt gtcactgaga
961ttgcttgtgg acggcagcac acttctgctt ttgttccttc atcaggacga atttactctt
1021ttgggcttgg tggtaatggg cagctgggaa ccggttcaac aagcaacagg aaaagcccct
1081ttactgtaaa aggaaattgg tacccctata atgggcagtg tctaccagat attgattctg
1141aagaatattt ctgtgtaaaa agaattttct cagggggaga tcaaagcttt tcacattact
1201ctagtcccca gaactgtggg ccaccagatg acttcagatg tcccaatccg acaaagcaga
1261tctggacagt gaatgaagct ctaattcaga aatggctgag ctatccttct ggaaggtttc
1321ctgtggagat agccaatgag atagatggaa cgttttcttc ctctggttgc ctaaatggaa
1381gttttttagc tgttagcaat gatgatcact atagaacagg taccagattt tcaggggttg
1441atatgaatgc tgctaggctt ttattccaca aacttataca acctgatcat ccgcagatat
1501ctcagcaggt ggcagctagt ttggaaaaga atcttattcc taaactgact agctccttac
1561ctgatgttga agcattgagg ttttatctta ctctaccaga atgtcccctg atgagtgatt
1621ccaacaattt cacaacaata gcaattccct ttggtacagc tcttgtgaac ctagaaaagg
1681caccactgaa agtacttgaa aactggtggt cagtacttga acctccacta ttcctcaaga
1741tagtagaact ttttaaggaa gttgtggtac atcttttgaa actctacaag atcggtattc
1801ccccttctga aagaagaatt ttcaacagtt ttcttcatac tgcattaaag gttttagaaa
1861tactacatag ggtaaatgag aaaatgggac agattataca gtatgataaa ttttatatac
1921atgaagtaca agaattgata gacataagaa atgattatat caactgggtc caacagcagg
1981cctatggaat gttggcagat atccctgtta caatctgtac atatccattt gtatttgatg
2041cccaagcaaa aactactctg ttacagaccg atgcagtctt acagatgcag atggctattg
2101atcaggccca caggcagaat gtctcctctc tttttctccc agtgattgaa tctgtgaatc
2161cctgcttaat tctagtggtg cgtagagaaa atattgtagg agatgcaatg gaagtcctta
2221ggaaaacaaa gaacatagat tacaagaagc cactcaaggt tatatttgtt ggagaagatg
2281ctgtggatgc aggaggggtg cgcaaagaat ttttcttgct catcatgagg gaattattgg
2341atcctaaata cggcatgttt aggtattatg aagattccag gctcatttgg ttttctgata
2401agacatttga agacagtgat ttgttccatt tgattggtgt tatctgtggc ttagcaattt
2461ataattgtac cattgtggac ctccattttc ctttggcttt atataagaaa ctactgaaaa
2521agaagccatc cttggatgat ttgaaagaac taatgcctga tgttgggaga agcatgcaac
2581agttactgga ttatccagaa gatgacatag aggaaacatt ttgtcttaat tttacgatca
2641cagttgaaaa ctttggtgca acagaagtga aagagctggt tctaaatggt gcagacacag
2701ctgttaacaa acaaaatcgg caagagtttg tcgatgctta tgtggattac atattcaata
2761aatcagtggc ttccttattt gatgcttttc atgcgggctt tcataaggtc tgtggaggaa
2821aagtccttct gctctttcag cctaatgaac tacaagcaat ggtcattGGA AATACAAATT
2881ATGATTGGAA GGAACTGGAA AAGAATACAG AATACAAAGG GGAATATTGG GCAGAACATC
2941CTACGATAAA AATTTTTTGG GAAGTATTTC ACGAATTACC ATTGGAAAAG AAGAAACAGT
3001TTCTGTTATT TTTGACAGGT AGTGATCGCA TTCCTATTCT TGGTATGAAG AGTCTGAAAC
3061TAGTCATCCA GTCCACAGGA GGTGGTGAGG AGTATCTCCC AGTTTCCCAT ACTTGTTTTA
3121ATCTTCTGGA TCTTCCAAAA TATACAGAAA AAGAAACTCT ACGCTCTAAA CTGATCCAAG
3181CTATTGATCA CAATGAAGGC TTCAGTTTAA TATACCCAAC TTTCTTGTAC AAAGTGGTTG
3241ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA
3301GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAGC
3361TTGAAGCTGG CGCGCCCGTG ATACGCGTTA AGTCgacaat caacctctgg attacaaaat
3421ttgtgaaaga tt

Download FASTA (ORF) (Full)