Construct: ORF TRCN0000470790

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015994.1_s317c1
Derived from:
ccsbBroadEn_10699
DNA Barcode:
CGAGAAGCCCGACGCTAGTATCCC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
BRCA1 (672)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000470790
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 672 BRCA1 BRCA1 DNA repair associated NM_007297.4 72.3% 72.1% (many diffs)
2 human 672 BRCA1 BRCA1 DNA repair associated NM_007300.4 71.6% 71.4% (many diffs)
3 human 672 BRCA1 BRCA1 DNA repair associated NM_007294.4 70.5% 70.3% (many diffs)
4 human 672 BRCA1 BRCA1 DNA repair associated NR_027676.2 55.2% (many diffs)
5 human 672 BRCA1 BRCA1 DNA repair associated NM_007298.3 47.9% 39.5% (many diffs)
6 human 672 BRCA1 BRCA1 DNA repair associated NM_007299.4 43.7% 33.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
4131
ORF length:
4062
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gataaatcag ggaactaacc aaacggagca gaatggtcaa gtgatgaata
121ttactaatag tggtcatgag aataaaacaa aaggtgattc tattcagaat gagaaaaatc
181ctaacccaat agaatcactc gaaaaagaat ctgctttcaa aacgaaagct gaacctataa
241gcagcagtat aagcaatatg gaactcgaat taaatatcca caattcaaaa gcacctaaaa
301agaataggct gaggaggaag tcttctacca ggcatattca tgcgcttgaa ctagtagtca
361gtagaaatct aagcccacct aattgtactg aattgcaaat tgatagttgt tctagcagtg
421aagagataaa gaaaaaaaag tacaaccaaa tgccagtcag gcacagcaga aacctacaac
481tcatggaagg taaagaacct gcaactggag ccaagaagag taacaagcca aatgaacaga
541caagtaaaag acatgacagt gatactttcc cagagctgaa gttaacaaat gcacctggtt
601cttttactaa gtgtccaaat accagtgaac ttaaagaatt tgtcaatcct agccttccaa
661gagaagaaaa agaagagaaa ctagaaacag ttaaagtgtc taataatgct gaagacccca
721aagatctcat gttaagtgga gaaagggttt tgcaaactga aagatctgta gagagtagca
781gtatttcact ggtacctggt actgattatg gcactcagga aagtatctcg ttactggaag
841ttagcactct agggaaggca aaaacagaac caaataaatg tgtgagtcag tgtgcagcat
901ttgaaaaccc caagggacta attcatggtt gttccaaaga taatagaaat gacacagaag
961gctttaagta tccattggga catgaagtta accacagtcg ggaaacaagc atagaaatgg
1021aagaaagtga actcgatgct cagtatttgc agaatacatt caaggtttca aagcgccagt
1081catttgctct gttttcaaat ccaggaaatg cagaagagga atgtgcaaca ttctctgccc
1141actctgggtc cttaaagaaa caaagtccaa aagtcacttt tgaatgtgaa caaaaggaag
1201aaaatcaagg aaagaatgag tctaatatca agcctgtaca gacagttaat atcactgcag
1261gctttcctgt ggttggtcag aaagataagc cagttgataa tgccaaatgt agtatcaaag
1321gaggctctag gttttgtcta tcatctcagt tcagaggcaa cgaaactgga ctcattactc
1381caaataaaca tggactttta caaaacccat atcgtatacc accacttttt cccatcaagt
1441catttgttaa aactaaatgt aagaaaaatc tgctagagga aaactttgag gaacattcaa
1501tgtcacctga aagagaaatg ggaaatgaga acattccaag tacagtgagc acaattagcc
1561gtaataacat tagagaaaat gtttttaaag gagccagctc aagcaatatt aatgaagtag
1621gttccagtac taatgaagtg ggctccagta ttaatgaaat aggttccagt gatgaaaaca
1681ttcaagcaga actaggtaga aacagagggc caaaattgaa tgctatgctt agattagggg
1741ttttgcaacc tgaggtctat aaacaaagtc ttcctggaag taattgtaag catcctgaaa
1801taaaaaagca agaatatgaa gaagtagttc agactgttaa tacagatttc tctccatatc
1861tgatttcaga taacttagaa cagcctatgg gaagtagtca tgcatctcag gtttgttctg
1921agacacctga tgacctgtta gatgatggtg aaataaagga agatactagt tttgctgaaa
1981atgacattaa ggaaagttct gctgttttta gcaaaagcgt ccagagagga gagcttagca
2041ggagtcctag ccctttcacc catacacatt tggctcaggg ttaccgaaga ggggccaaga
2101aattagagtc ctcagaagag aacttatcta gtgaggatga agagcttccc tgcttccaac
2161acttgttatt tggtaaagta aacaatatac cttctcagtc tactaggcat agcaccgttg
2221ctaccgagtg tctgtctaag aacacagagg agaatttatt atcattgaag aatagcttaa
2281atgactgcag taaccaggta atattggcaa aggcatctca ggaacatcac cttagtgagg
2341aaacaaaatg ttctgctagc ttgttttctt cacagtgcag tgaattggaa gacttgactg
2401caaatacaaa cacccaggat cctttcttga ttggttcttc caaacaaatg aggcatcagt
2461ctgaaagcca gggagttggt ctgagtgaca aggaattggt ttcagatgat gaagaaagag
2521gaacgggctt ggaagaaaat aatcaagaag agcaaagcat ggattcaaac ttaggtgaag
2581cagcatctgg gtgtgagagt gaaacaagcg tctctgaaga ctgctcaggg ctatcctctc
2641agagtgacat tttaaccact cagcagaggg ataccatgca acataacctg ataaagctcc
2701agcaggaaat ggctgaacta gaagctgtgt tagaacagca tgggagccag ccttctaaca
2761gctacccttc catcataagt gactcctctg cccttgagga cctgcgaaat ccagaacaaa
2821gcacatcaga aaaagattcg catatacatg gccaaaggga caactccatg ttttctaaaa
2881ggcctagaga acatatatca gtattaactt cacagaaaag tagtgaatac cctataagcc
2941agaatccaga aggcctttct gctgacaagt ttgaggtgtc tgcagatagt tctaccagta
3001aaaataaaga accaggagtg gaaaggtcat ccccttctaa atgcccatca ttagatgata
3061ggtggtacat gcacagttgc tctgggagtc ttcagaatag aaactaccca tctcaagagg
3121ggctcattaa ggttgttgat gtggaggagc aacagctgga agagtctggg ccacacgatt
3181tgacggaaac atcttacttg ccaaggcaag atctagaggg aaccccttac ctggaatctg
3241gaatcagcct cttctctgat gaccctgaat ctgatccttc tgaagacaga gccccagagt
3301cagctcgtgt tggcaacata ccatcttcaa cctctgcatt gaaagttccc caattgaaag
3361ttgcagaatc tgcccagggt ccagctgctg ctcatactac tgatactgct gggtataatg
3421caatggaaga aagtgtgagc agggagaagc cagaattgac agcttcaaca gaaagggtca
3481acaaaagaat gtccatagtg gtgtctggcc tgaccccaga agaatttatg ctcgtgtaca
3541agtttgccag aaaacaccac atcactttaa ctaatctaat tactgaagag actactcatg
3601ttgttatgaa aacagatgct gagtttgtgt gtgaacggac actgaaatat tttctaggaa
3661ttgcgggagg aaaatgggta gttagctatt tctgGGTGAC CCAGTCTATT AAAGAAAGAA
3721AAATGCTGAA TGAGCATGAT TTTGAAGTCA GAGGAGATGT GGTCAATGGA AGAAACCACC
3781AAGGTCCAAA GCGAGCAAGA GAATCCCAGG ACAGAAAGAT CTTCAGGGGG CTAGAAATCT
3841GTTGCTATGG GCCCTTCACC AACATGCCCA CAGATCAACT GGAATGGATG GTACAGCTGT
3901GTGGTGCTTC TGTGGTGAAG GAGCTTTCAT CATTCACCCT TGGCACAGGT GTCCACCCAA
3961TTGTGGTTGT GCAGCCAGAT GCCTGGACAG AGGACAATGG CTTCCATGCA ATTGGGCAGA
4021TGTGTGAGGC ACCTGTGGTG ACCCGAGAGT GGGTGTTGGA CAGTGTAGCA CTCTACCAGT
4081GCCAGGAGCT GGACACCTAC CTGATACCCC AGATCCCCCA CAGCCACTAC TTGCCAACTT
4141TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT
4201CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT
4261CTTGTGGAAA GGACGACGAG AAGCCCGACG CTAGTATCCC ACGCGTTAAG TCgacaatca
4321acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)