Construct: ORF TRCN0000470827
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013559.1_s317c1
- Derived from:
- ccsbBroadEn_01362
- DNA Barcode:
- GAGCAAGATAAGCATAAGCAACTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RAB27B (5874)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470827
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5874 | RAB27B | RAB27B, member RAS oncogene... | NM_004163.4 | 100% | 100% | |
| 2 | human | 5874 | RAB27B | RAB27B, member RAS oncogene... | XM_005266733.1 | 100% | 100% | |
| 3 | human | 5874 | RAB27B | RAB27B, member RAS oncogene... | XM_017025913.1 | 100% | 100% | |
| 4 | human | 5874 | RAB27B | RAB27B, member RAS oncogene... | XM_017025914.1 | 100% | 100% | |
| 5 | human | 5874 | RAB27B | RAB27B, member RAS oncogene... | XM_024451232.1 | 100% | 100% | |
| 6 | human | 5874 | RAB27B | RAB27B, member RAS oncogene... | XM_006722518.1 | 78.4% | 73.8% | (many diffs) |
| 7 | human | 5874 | RAB27B | RAB27B, member RAS oncogene... | XM_011526131.1 | 78.4% | 73.8% | (many diffs) |
| 8 | human | 5874 | RAB27B | RAB27B, member RAS oncogene... | XR_935245.1 | 11.1% | 1_521del;1176_5858del | |
| 9 | mouse | 80718 | Rab27b | RAB27B, member RAS oncogene... | NM_001082553.2 | 88.9% | 94.4% | (many diffs) |
| 10 | mouse | 80718 | Rab27b | RAB27B, member RAS oncogene... | NM_001302798.1 | 88.9% | 94.4% | (many diffs) |
| 11 | mouse | 80718 | Rab27b | RAB27B, member RAS oncogene... | NM_030554.4 | 88.9% | 94.4% | (many diffs) |
| 12 | mouse | 80718 | Rab27b | RAB27B, member RAS oncogene... | NM_001302799.1 | 61.3% | 61% | (many diffs) |
| 13 | mouse | 80718 | Rab27b | RAB27B, member RAS oncogene... | XM_006526364.2 | 61.3% | 61% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 720
- ORF length:
- 654
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac cgatggagac tatgattatc tgatcaaact cctggccctc ggggattcag 121 gggtggggaa gacaacattt ctttatagat acacagataa taaattcaat cccaaattca 181 tcactacagt aggaatagac tttcgggaaa aacgtgtggt ttataatgca caaggaccga 241 atggatcttc agggaaagca tttaaagtgc atcttcagct ttgggacact gcgggacaag 301 agcggttccg gagtctcacc actgcatttT TCAGAGACGC CATGGGCTTC TTATTAATGT 361 TTGACCTCAC CAGTCAACAG AGCTTCTTAA ATGTCAGAAA CTGGATGAGC CAACTGCAAG 421 CAAATGCTTA TTGTGAAAAT CCAGATATAG TATTAATTGG CAACAAGGCA GACCTACCAG 481 ATCAGAGGGA AGTCAATGAA CGGCAAGCTC GGGAACTGGC TGACAAATAT GGCATACCAT 541 ATTTTGAAAC AAGTGCAGCA ACTGGACAGA ATGTGGAGAA AGCTGTAGAA ACCCTTTTGG 601 ACTTAATCAT GAAGCGAATG GAACAGTGTG TGGAGAAGAC ACAAATCCCT GATACTGTCA 661 ATGGTGGAAA TTCTGGAAAC TTGGATGGGG AAAAGCCACC AGAGAAGAAA TGTATCTGCT 721 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 781 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 841 TTTATATATC TTGTGGAAAG GACGAGAGCA AGATAAGCAT AAGCAACTAA CGCGTTAAGT 901 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt