Construct: ORF TRCN0000470846
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008761.1_s317c1
- Derived from:
- ccsbBroadEn_04860
- DNA Barcode:
- AAGGCAACAGCAAATGCTGTGGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C2orf76 (130355)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470846
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 130355 | C2orf76 | chromosome 2 open reading f... | NM_001017927.3 | 100% | 100% | |
| 2 | human | 130355 | C2orf76 | chromosome 2 open reading f... | NM_001322329.1 | 100% | 100% | |
| 3 | human | 130355 | C2orf76 | chromosome 2 open reading f... | NM_001322330.1 | 100% | 100% | |
| 4 | human | 130355 | C2orf76 | chromosome 2 open reading f... | NM_001322331.2 | 100% | 100% | |
| 5 | human | 130355 | C2orf76 | chromosome 2 open reading f... | NM_001322332.1 | 86.5% | 86.5% | 132_133ins51 |
| 6 | human | 130355 | C2orf76 | chromosome 2 open reading f... | XM_017003354.2 | 79.2% | 79.2% | 304_402del |
| 7 | human | 130355 | C2orf76 | chromosome 2 open reading f... | XR_001738623.2 | 48.4% | (many diffs) | |
| 8 | mouse | 73103 | 3110009E18Rik | RIKEN cDNA 3110009E18 gene | NM_028439.2 | 86.2% | 83.3% | (many diffs) |
| 9 | mouse | 73103 | 3110009E18Rik | RIKEN cDNA 3110009E18 gene | XM_017312452.1 | 82.3% | 79.5% | (many diffs) |
| 10 | mouse | 73103 | 3110009E18Rik | RIKEN cDNA 3110009E18 gene | NM_001172074.1 | 58.7% | 48.2% | (many diffs) |
| 11 | mouse | 73103 | 3110009E18Rik | RIKEN cDNA 3110009E18 gene | XM_017312453.1 | 56.4% | 46.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 447
- ORF length:
- 378
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggctcctGGA GAAGTGACCA TCACAGTTCG CCTCATCCGT TCCTTTGAAC 121 ATCGCAATTT CAAACCTGTA GTGTATCACG GAGTGAATTT GGACCAAACT GTAAAGGAAT 181 TTATCGTATT TCTAAAGCAA GATATCCCTT TAAGGACCAA CCTGCCACCA CCATTCAGAA 241 ATTATAAATA TGATGCACTA AAGATTATTC ATCAAGCACA TAAATCAAAG ACAAATGAAC 301 TTGTGTTGAG TTTGGAAGAT GACGAAAGAC TCCTGCTGAA AGAAGACAGC ACTCTGAAAG 361 CAGCTGGAAT CGCCAGTGAA ACTGAAATTG CATTCTTCTG TGAAGAAGAT TATAAGAACT 421 ACAAAGCTAA TCCCATTTCA TCCTGGTTGC CAACTTTCTT GTACAAAGTG GTTGATATCG 481 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 541 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAAAGGCAAC 601 AGCAAATGCT GTGGCGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 661 aagatt