Construct: ORF TRCN0000470862
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009315.1_s317c1
- Derived from:
- ccsbBroadEn_06170
- DNA Barcode:
- CCTTATTTCTCCCTATTGCCCGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EPHA8 (2046)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470862
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2046 | EPHA8 | EPH receptor A8 | NM_001006943.3 | 99.9% | 100% | 6C>T |
| 2 | human | 2046 | EPHA8 | EPH receptor A8 | XR_946576.2 | 62.7% | (many diffs) | |
| 3 | human | 2046 | EPHA8 | EPH receptor A8 | NM_020526.5 | 47.7% | 43.9% | (many diffs) |
| 4 | human | 2046 | EPHA8 | EPH receptor A8 | XM_011540969.1 | 40.3% | 36.6% | (many diffs) |
| 5 | human | 2046 | EPHA8 | EPH receptor A8 | XM_011540970.1 | 40.3% | 36.6% | (many diffs) |
| 6 | human | 2046 | EPHA8 | EPH receptor A8 | XM_011540972.1 | 40.3% | 36.6% | (many diffs) |
| 7 | human | 2046 | EPHA8 | EPH receptor A8 | XM_011540973.1 | 40.3% | 36.6% | (many diffs) |
| 8 | mouse | 13842 | Epha8 | Eph receptor A8 | XM_006538530.3 | 79.6% | 84.8% | (many diffs) |
| 9 | mouse | 13842 | Epha8 | Eph receptor A8 | NM_007939.2 | 43.2% | 42.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1551
- ORF length:
- 1485
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tcccgcccgg ggccgcctgc cccctgcgct ctgggtcgtc acggccgcgg 121 cggcggcggc cacctgcgtg tccgcggcgc gcggcgaagt gaatttgctg gacacgtcga 181 ccatccacgg ggactggggc tggctcacgt atccggctca tgggtgggac tccatcaacg 241 aggtggacga gtccttccag cccatccaca cgtaccaggt ttgcaacgtc atgagcccca 301 accagaacaa ctggctgcgc acgagctggg tcccccgaga cggcgcccgg cgcgtctatg 361 ctgagatcaa gtttaccctg cgcgactgca acagcatgcc tggtgtgctg ggcacctgca 421 aggagacctt caacctctac tacctggagt cggaccgcga cctgggggcc agcacacaag 481 aaagccagtt cctcaaaatc gacaccattg cggccgacga gagcttcaca ggtgccgacc 541 ttggtgtgcg gcgtctcaag ctcaacacgg aggtgcgcag tgtgggtccc ctcagcaagc 601 gcggcttcta cctggccttc caggacatag gtgcctgcct ggccatcctc tctctccgca 661 tctactataa gaagtgccct gccatggtgc gcaatctggc tgccttctcg gaggcagtga 721 cgggggccga ctcgtcctca ctggtggagg tgaggggcca gtgcgtgcgg cactcagagg 781 agcgggacac acccaagatg tactgcagcg cggagggcga gtggctcgtg cccatcggca 841 aatgcgtgtg cagtgccggc tacgaggagc ggcgggatgc ctgtgtggcc tgtgagctgg 901 gcttctacaa gtcagcccct ggggaccagc tgtgtgcccg ctgccctccc cacagccact 961 ccgcagctcc agccgcccaa gcctgccact gtgacctcag ctactaccgt gcagccctgg 1021 acccgccgtc ctcagcctgc acccggccac cctcggcacc agtgaacctg atctccagtg 1081 tgaatgggac atcagtgact ctggagtggg cccctcccct ggacccaggt ggccgcagtg 1141 acaTCACCTA CAATGCCGTG TGCCGCCGCT GCCCCTGGGC ACTGAGCCGC TGCGAGGCAT 1201 GTGGGAGCGG CACCCGCTTT GTGCCCCAGC AGACAAGCCT GGTGCAGGCC AGCCTGCTGG 1261 TGGCCAACCT GCTGGCCCAC ATGAACTACT CCTTCTGGAT CGAGGCCGTC AATGGCGTGT 1321 CCGACCTGAG CCCCGAGCCC CGCCGGGCCG CTGTGGTCAA CATCACCACG AACCAGGCAG 1381 GTAGGCGGAG AAACTCCGTC CCGCAGCGTC CTGGTCCCCC AGCTTCCCCT GCCTCAGACC 1441 CATCCAGGGA TCAGAGCTCT GCCGGGGACG TGCTGTGGGC CTTTAGGCAA GTGCCTCTCT 1501 GGCCCTGCGC TCCTCACCAG GACCCAGAGC TGGAGGCTCT TCATTGCCTT TACCCAACTT 1561 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1621 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1681 CTTGTGGAAA GGACGACCTT ATTTCTCCCT ATTGCCCGTC ACGCGTTAAG TCgacaatca 1741 acctctggat tacaaaattt gtgaaagatt