Construct: ORF TRCN0000470889
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005587.1_s317c1
- Derived from:
- ccsbBroadEn_09783
- DNA Barcode:
- TGCTCACCGTACAGGGACGCAGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ADAMTS18 (170692)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470889
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 170692 | ADAMTS18 | ADAM metallopeptidase with ... | NM_199355.4 | 99.8% | 99.7% | (many diffs) |
| 2 | human | 170692 | ADAMTS18 | ADAM metallopeptidase with ... | NM_001326358.2 | 85.6% | 76.9% | (many diffs) |
| 3 | human | 170692 | ADAMTS18 | ADAM metallopeptidase with ... | XM_011522923.1 | 85.6% | 76.9% | (many diffs) |
| 4 | human | 170692 | ADAMTS18 | ADAM metallopeptidase with ... | XM_011522924.2 | 79.7% | 71.3% | (many diffs) |
| 5 | human | 170692 | ADAMTS18 | ADAM metallopeptidase with ... | XM_017022988.2 | 66.1% | 66% | (many diffs) |
| 6 | human | 170692 | ADAMTS18 | ADAM metallopeptidase with ... | XM_017022989.1 | 66.1% | 66% | (many diffs) |
| 7 | mouse | 208936 | Adamts18 | a disintegrin-like and meta... | NM_172466.3 | 85.3% | 88.5% | (many diffs) |
| 8 | mouse | 208936 | Adamts18 | a disintegrin-like and meta... | XM_006530795.4 | 83% | 86.1% | (many diffs) |
| 9 | mouse | 208936 | Adamts18 | a disintegrin-like and meta... | XM_006530797.2 | 78% | 76.8% | (many diffs) |
| 10 | mouse | 208936 | Adamts18 | a disintegrin-like and meta... | XM_006530800.2 | 73% | 70.7% | (many diffs) |
| 11 | mouse | 208936 | Adamts18 | a disintegrin-like and meta... | XM_006530801.3 | 73% | 70.7% | (many diffs) |
| 12 | mouse | 208936 | Adamts18 | a disintegrin-like and meta... | XR_878788.2 | 63.3% | (many diffs) | |
| 13 | mouse | 208936 | Adamts18 | a disintegrin-like and meta... | XM_030243369.1 | 56.7% | 59.7% | (many diffs) |
| 14 | mouse | 208936 | Adamts18 | a disintegrin-like and meta... | XM_030243370.1 | 56.7% | 59.7% | (many diffs) |
| 15 | mouse | 208936 | Adamts18 | a disintegrin-like and meta... | XR_001778415.2 | 51.2% | (many diffs) | |
| 16 | mouse | 208936 | Adamts18 | a disintegrin-like and meta... | XM_017312631.1 | 49.8% | 47.7% | (many diffs) |
| 17 | mouse | 208936 | Adamts18 | a disintegrin-like and meta... | XR_001778416.1 | 41.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3729
- ORF length:
- 3663
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gtgcgccctc ctgctcgcgt gtgccttccc ggctgcgggt tcgggcccgc 121 cgaggggcct ggcgggactg gggcgcgtgg ccaaggcgct ccagctgtgc tgcctctgct 181 gtgcgtcggt cgccgcggcc ttagccagtg acagcagcag cggcgccagc ggattaaatg 241 atgattacgt ctttgtcacg ccagtagaag tagactcagc cgggtcatat atttcacacg 301 acattttgca caacggcagg aaaaagcgat cggcgcagaa tgccagaagc tccctgcact 361 accgattttc agcatttgga caggaactgc acttagaact taagccctcg gcgattttga 421 gcagtcactt tattgtccag gtacttggaa aagatggtgc ttcagagact cagaaacccg 481 aggtgcagca atgcttctat cagggattta tcagaaatga cagctcctcc tctgtcgctg 541 tgtctacgtg tgctggcttg tcaggtttaa taaggacacg aaaaaatgaa ttcctcatct 601 cgccattacc tcagcttctg gcccaggaac acaaccacag ctcccctgcg ggtcaccatc 661 ctcacgtact gtacaaaagg acagcagagg agaagatcca gcggtaccgt ggctaccccg 721 gctctggccg gaattatcct ggttactccc caagtcacat tccccatgca tctcagagtc 781 gagagacaga gtatcaccat cgaaggttgc aaaagcagca tttttgtgga cgacgcaaga 841 aatatgctcc caagcctccc acagaggaca cctatctaag gtttgatgaa tatgggagct 901 ctgggcgacc cagaagatca gctggaaaat cacaaaaggg cctcaatgtg gaaaccctcg 961 tggtggcaga caagaaaatg gtggaaaagc atggcaaggg aaatgtcacc acatacattc 1021 tcacagtaat gaacatggtt tctggcctat ttaaagatgg gactattgga agtgacataa 1081 acgtggttgt ggtgagccta attcttctgg aacaagaacc tggaggatta ttgatcaacc 1141 atcatgcaga ccagtctctg aatagttttt gtcaatggca gtctgccctc attggaaaga 1201 atggcaagag acatgatcat gccatcttac taacaggatt tgatatttgt tcttggaaga 1261 atgaaccatg tgacactcta gggtttgccc ccatcagtgg aatgtgctct aagtaccgaa 1321 gttgtaccat caatgaggac acaggacttg gccttgcctt caccatcgct catgagtcag 1381 ggcacaactt tggtatgatt cacgacggag aagggaatcc ctgcagaaag gctgaaggca 1441 atatcatgtc tcccacactg accggaaaca atggagtgtt ttcatggtct tcctgcagcc 1501 gccagtatct caagaaattc ctcagcacac ctcaggcggg gtgtctagtg gatgagccca 1561 agcaagcagg acagtataaa tatccggaca aactaccagg acagatttat gatgctgaca 1621 cacagtgtaa atggcaattt ggagcaaaag ccaagttatg cagccttggt tttgtgaagg 1681 atatttgcaa atcactttgg tgccaccgag taggccacag gtgtgagacc aagtttatgc 1741 ccgcagcaga agggaccgtt tgtggcttga gtatgtggtg tcggcaaggc cagtgcgtaa 1801 agtttgggga gctcgggccc cggcccatcc acggccagtg gtccgcctgg tcgaagtggt 1861 cagaatgttc ccggacatgt ggtggaggag tcaagttcca ggagagacac tgcaataacc 1921 ccaagcctca gtatggtggc atattctgtc caggttctag ccgtatttat cagctgtgca 1981 atattaaccc ttgcaatgaa aatagcttgg attttcgggc tcaacagtgt gcagaatata 2041 acagcaaacc tttccgtgga tggttctacc agtggaaacc ctatacaaaa gtggaagagg 2101 aagatcgatg caaactgtac tgcaaggctg agaactttga attttttttt gcaatgtccg 2161 gcaaagtgaa agatggaact ccctgctccc caaacaaaaa tgatgtttgt attgacgggg 2221 tttgtgaact agtgggatgt gatcatgaac taggctctaa agcagtttca gatgcttgtg 2281 gcgtttgcaa aggtgataat tcaacttgca agttttataa aggcctgtac ctcaaccagc 2341 ataaagcaaa tgaatattat ccggtggtca tcattccagc tggcgcccga agcatcgaaa 2401 tccaggagct gcaggtttcc tccagttacc tcgcagttcg aagcctcagt caaaagtatt 2461 acctcaccgg gggctggagc atcgactggc ctggggagtt ccccttcgct gggaccacgt 2521 ttgaatacca gcgctctttc aaccgcccgg aacgtctgta cgcgccaggg cccacaaatg 2581 agacgctggt ctttgaaatt ctgatgcaag gcaaaaatcc agggatagct tggaagtatg 2641 cacttcccaa ggtcatgaat ggaactccac cagccacaaa aagacctgcc tatacctgga 2701 gtatcgtgca gtcagagtgc tccgtctcct gtggtggagg ttacataaat gtaaaggcca 2761 tttgcttgcg agatcaaaat actcaagtca attcctcatt ctgcagtgca aaaaccaagc 2821 cagtaactga gcccaaaatc tgcaacgctt tctcctgccc ggcttactgg atgccaggtg 2881 aatggagtac atgcagcaag gcctgtgctg gaggccagca gagccgaaag atccagtgtg 2941 tgcaaaagaa gcccttccaa aaggaggaag cagtgttgca ttctctctgt ccagtgagca 3001 cacccactca ggtccaagcc tgcaacagcc atgcctgccc tccacaatgg agccttggac 3061 cctggtctca gtgttccaag acctgtggac gaggggtgag gaagcgtgaa ctcctctgca 3121 agggctctgc cgcagaaacc ctccccgaga gccagtgtac cagtctcccc agacctgagc 3181 tgcaggaggg ctgtgtgctt ggacgatgcc ccaagaacag ccggctacag tgggtcgcTT 3241 CTTCGTGGAG CGAGTGTTCT GCAACCTGTG GTTTGGGTGT GAGGAAGAGG GAGATGAAGT 3301 GCAGCGAGAA GGGCTTCCAG GGAAAGCTGA TAACTTTCCC AGAGCGAAGA TGCCGTAATA 3361 TTAAGAAACC AAATCTGGAC TTGGAAGAGA CCTGCAACCG ACGGGCTTGC CCAGCCCATC 3421 CAGTGTACAA CATGGTAGCT GGATGGTATT CATTGCCGTG GCAGCAGTGC ACAGTCACCT 3481 GTGGGGGAGG GGTCCAGACC CGGTCAGTCC ACTGTGTTCA GCAAGGCCGG CCTTCCTCAA 3541 GTTGTCTGCT CCATCAGAAA CCTCCGGTGC TACGAGCCTG TAATACAAAC TTCTGTCCAG 3601 CTCCTGAAAA GAGAGAGGAT CCATCCTGCG TAGATTTCTT CAACTGGTGT CACCTAGTTC 3661 CTCAGCATGG TGTCTGCAAC CACAAGTTTT ACGGAAAACA ATGCTGCAAG TCATGCACAA 3721 GGAAGATCTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 3781 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 3841 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATGCTCA CCGTACAGGG ACGCAGACAC 3901 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt