Construct: ORF TRCN0000470892
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005495.1_s317c1
- Derived from:
- ccsbBroadEn_02669
- DNA Barcode:
- GGTATCGAACGTACGTCATTGACT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- VPS45 (11311)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470892
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11311 | VPS45 | vacuolar protein sorting 45... | NM_007259.5 | 100% | 100% | |
| 2 | human | 11311 | VPS45 | vacuolar protein sorting 45... | NM_001279354.2 | 93.6% | 93.6% | 0_1ins108 |
| 3 | human | 11311 | VPS45 | vacuolar protein sorting 45... | XM_024452791.1 | 93.6% | 93.6% | 0_1ins108 |
| 4 | human | 11311 | VPS45 | vacuolar protein sorting 45... | XR_921734.3 | 73.7% | 1_86del;1189_1256del;1865_2318del | |
| 5 | human | 11311 | VPS45 | vacuolar protein sorting 45... | NM_001279353.2 | 72.3% | 67.6% | (many diffs) |
| 6 | human | 11311 | VPS45 | vacuolar protein sorting 45... | XR_921733.3 | 70.5% | 1_86del;1580_1810del;2028_2423del | |
| 7 | human | 11311 | VPS45 | vacuolar protein sorting 45... | NR_103998.2 | 58.7% | (many diffs) | |
| 8 | mouse | 22365 | Vps45 | vacuolar protein sorting 45 | NM_013841.3 | 90.8% | 97% | (many diffs) |
| 9 | mouse | 22365 | Vps45 | vacuolar protein sorting 45 | XM_011240074.1 | 59.3% | 63.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1776
- ORF length:
- 1710
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa cgtggttttt gctgtgaagc agtacatttc caaaatgata gaggacagcg 121 ggcctggtat gaaagtactt ctcatggata aagagacgac tggcatagtg agtatggtat 181 acacacaatc ggagattcta cagaaggaag tgtacctctt tgaacgcatt gattctcaaa 241 atcgagagat catgaaacac ctgaaggcaa tttgttttct tcgacctaca aaggagaatg 301 tggattatat tattcaggag ctccgaagac ccaaatacac tatatatttc atttatttca 361 gtaatgtgat cagcaagagt gacgtgaagt cattggctga agctgatgaa caggaagttg 421 tggctgaggt tcaggaattt tatggtgatt acattgctgt gaacccacat ttgttttccc 481 tcaatatttt gggttgctgc cagggtcgaa attgggatcc agcccagcta tctagaacaa 541 ctcaagggct tacagctctc cttttatctc tgaagaagtg tcccatgatt cgttatcagc 601 tctcatcaga ggcagcaaag agacttgcag agtgcgttaa gcaagtgata actaaagaat 661 atgaactgtt tgaattccgt cggacagagg ttcctccatt gctccttatt ttagatcgct 721 gtgatgatgc catcacccca ttgctaaacc agtggacata tcaggccatg gtccacgaac 781 tactaggcat aaacaacaat cggattgatc tttccagagt gccgggaatc agtaaagact 841 taagagaagt ggtcctatct gctgaaaatg atgaattcta tgctaataat atgtacctga 901 actttgctga gattggtagc aatataaaga atctcatgga agattttcag aagaagaaac 961 caaaagaaca gcaaaaacta gaatcaatag cagacatgaa ggcgtttgtt gagaattatc 1021 cacagttcaa gaaaatgtct gggactgttt caaagcatgt gacagtggtt ggagaactgt 1081 ctcgattggt cagtgaacgg aatctgctgg aggtttcaga ggttgagcaa gaactggcct 1141 gtcaaaatga ccattctagt gctctccaga atataaaaag gcttctgcag aaccccaaag 1201 tgacagagtt tgatgctgcc cgcctggtga tgctttatgc tttacattat gagcgacaca 1261 gcagcaatag cctgccagga ctaatgatgg accTCAGGAA TAAAGGTGTT TCTGAGAAGT 1321 ATCGAAAGCT CGTGTCTGCA GTTGTTGAAT ATGGTGGTAA ACGAGTCAGA GGAAGTGACC 1381 TCTTCAGCCC CAAAGATGCT GTGGCTATCA CCAAACAATT CCTCAAAGGA CTGAAGGGAG 1441 TAGAAAATGT ATATACACAG CATCAACCTT TCCTACATGA AACCCTGGAT CATCTCATCA 1501 AAGGAAGGCT TAAGGAAAAC CTATATCCTT ATTTAGGCCC CAGCACACTC AGAGACAGAC 1561 CTCAGGATAT CATTGTGTTT GTAATTGGAG GAGCCACCTA TGAAGAGGCT CTAACAGTTT 1621 ATAACCTGAA CCGCACCACT CCTGGAGTGA GGATTGTCCT GGGAGGCACC ACAGTGCACA 1681 ACACGAAAAG TTTCCTAGAG GAAGTTCTGG CTTCTGGACT GCACAGCCGA AGCAAGGAGA 1741 GCTCTCAAGT CACATCAAGG TCAGCGAGCA GAAGATGCCC AACTTTCTTG TACAAAGTGG 1801 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1861 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1921 AGGTATCGAA CGTACGTCAT TGACTACGCG TTAAGTCgac aatcaacctc tggattacaa 1981 aatttgtgaa agatt