Transcript: Mouse NM_013841.3

Mus musculus vacuolar protein sorting 45 (Vps45), mRNA.

Source:
NCBI, updated 2017-05-27
Taxon:
Mus musculus (mouse)
Gene:
Vps45 (22365)
Length:
2643
CDS:
682..2394

Additional Resources:

NCBI RefSeq record:
NM_013841.3
NBCI Gene record:
Vps45 (22365)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_013841.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000093295 CCGAGATTGGTAGCAATATAA pLKO.1 1523 CDS 100% 15.000 21.000 N Vps45 n/a
2 TRCN0000324438 CCGAGATTGGTAGCAATATAA pLKO_005 1523 CDS 100% 15.000 21.000 N Vps45 n/a
3 TRCN0000093296 GCGGTGAATCCACATTTGTTT pLKO.1 1072 CDS 100% 5.625 4.500 N Vps45 n/a
4 TRCN0000324436 GCGGTGAATCCACATTTGTTT pLKO_005 1072 CDS 100% 5.625 4.500 N Vps45 n/a
5 TRCN0000093297 CCTCAGGATATCATCGTGTTT pLKO.1 2176 CDS 100% 4.950 3.960 N Vps45 n/a
6 TRCN0000324437 CCTCAGGATATCATCGTGTTT pLKO_005 2176 CDS 100% 4.950 3.960 N Vps45 n/a
7 TRCN0000093298 CTGCTCAACCAGTGGACATAT pLKO.1 1357 CDS 100% 13.200 9.240 N Vps45 n/a
8 TRCN0000324366 CTGCTCAACCAGTGGACATAT pLKO_005 1357 CDS 100% 13.200 9.240 N Vps45 n/a
9 TRCN0000093294 GAATGTCATGGCCTCCTCTTA pLKO.1 2416 3UTR 100% 4.950 3.465 N Vps45 n/a
10 TRCN0000324365 GAATGTCATGGCCTCCTCTTA pLKO_005 2416 3UTR 100% 4.950 3.465 N Vps45 n/a
11 TRCN0000157386 GCCAGGAATCAGCAAAGACTT pLKO.1 1437 CDS 100% 4.950 3.465 N VPS45 n/a
12 TRCN0000156836 GATCAGCAAGAGTGACGTGAA pLKO.1 984 CDS 100% 4.050 2.835 N VPS45 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_013841.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02669 pDONR223 100% 90.8% 97% None (many diffs) n/a
2 ccsbBroad304_02669 pLX_304 0% 90.8% 97% V5 (many diffs) n/a
3 TRCN0000470892 GGTATCGAACGTACGTCATTGACT pLX_317 28.4% 90.8% 97% V5 (many diffs) n/a
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