Construct: ORF TRCN0000470941
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003395.1_s317c1
- Derived from:
- ccsbBroadEn_09022
- DNA Barcode:
- TAGCAGGATTGGTCCTCGGCACAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC35F5 (80255)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470941
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80255 | SLC35F5 | solute carrier family 35 me... | NM_025181.5 | 99.9% | 100% | 1563A>C |
| 2 | human | 80255 | SLC35F5 | solute carrier family 35 me... | NM_001330314.2 | 98.2% | 97.7% | (many diffs) |
| 3 | human | 80255 | SLC35F5 | solute carrier family 35 me... | NM_001330315.2 | 97.5% | 97.3% | 921_959del;1602A>C |
| 4 | human | 80255 | SLC35F5 | solute carrier family 35 me... | XM_017005027.1 | 97.5% | 97.3% | 921_959del;1602A>C |
| 5 | human | 80255 | SLC35F5 | solute carrier family 35 me... | NM_001330316.2 | 96.5% | 95.8% | (many diffs) |
| 6 | human | 80255 | SLC35F5 | solute carrier family 35 me... | XM_011511922.2 | 95.8% | 95.1% | (many diffs) |
| 7 | human | 80255 | SLC35F5 | solute carrier family 35 me... | XM_011511923.2 | 94.2% | 93.3% | (many diffs) |
| 8 | human | 80255 | SLC35F5 | solute carrier family 35 me... | NM_001330317.2 | 46.5% | 45.6% | (many diffs) |
| 9 | human | 80255 | SLC35F5 | solute carrier family 35 me... | NR_104470.3 | 14.4% | (many diffs) | |
| 10 | mouse | 74150 | Slc35f5 | solute carrier family 35, m... | NM_028787.4 | 88.4% | 94% | (many diffs) |
| 11 | mouse | 74150 | Slc35f5 | solute carrier family 35, m... | XM_006529910.3 | 84.3% | 88.3% | (many diffs) |
| 12 | mouse | 74150 | Slc35f5 | solute carrier family 35, m... | XM_006529912.3 | 81.1% | 87.2% | (many diffs) |
| 13 | mouse | 74150 | Slc35f5 | solute carrier family 35, m... | XR_001778392.1 | 47% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1635
- ORF length:
- 1569
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt gccgccacga cgccatcgcg gggcaggaag gccaggggtg ctgagttctt 121 cacctccttt tagactgaga tctgccaagt tttccggcat tgctcttgag gatctcagaa 181 gggctcttaa gacaagactg caaatggtgt gtgtatttgt catgaaccga atgaattccc 241 agaacagtgg tttcactcag cgcaggcgaa tggctcttgg gattgttatt cttctgcttg 301 ttgatgtgat atgggttgct tcctctgaac ttacttcgta tgtttttacc cagtacaaca 361 aaccattctt cagcaccttt gcaaaaacat ctatgtttgt tttgtacctt ttgggcttta 421 ttatttggaa gccatggaga caacagtgta caagaggact tcgcggaaag catgctgctt 481 tttttgcaga tgctgaaggt tactttgctg cttgcacaac agatacaact atgaatagtt 541 ctttgagtga acctctgtat gtgcctgtga aattccatga tcttccaagt gaaaaacctg 601 agagcacaaa cattgatact gaaaaaaccc ccaaaaagtc tcgtgtgagg ttcagtaata 661 tcatggagat tcgacagctt ccgtcaagtc atgcattgga agcaaagttg tctcgcatgt 721 catatcctgt gaaagaacaa gaatccatac tgaaaactgt ggggaaactt actgcaactc 781 aagtagcgaa aattagcttt tttttttgct ttgtgtggtt tttggcaaat ttgtcatatc 841 aagaagcact ttcagacaca caagttgcta tagttaatat tttatcttca acttccggac 901 tttttacctt aatccttgct gcagtatttc caagtaacag tggagataga tttacccttt 961 ctaaactatt agctgtaatt ttaagcattg gaggcgttgt actggtaaac ctggcagggt 1021 ctgaaaaacc tgctggaaga gacacagtag gttccatttg gtctcttgct ggagccatgc 1081 tctatgctgt ctatattgtt atgattaaga gaaaagtaga tagagaagac aagttggata 1141 ttccaatgtt ctttggtttt gtaggtttgt ttaatctgct gctcttatgg ccagGTTTCT 1201 TTTTACTTCA TTATACTGGA TTTGAGGACT TCGAGTTTCC CAATAAAGTA GTATTAATGT 1261 GCATTATCAT TAATGGCCTT ATTGGAACAG TACTCTCAGA GTTCCTGTGG TTGTGGGGCT 1321 GCTTTCTTAC CTCATCATTG ATAGGCACAC TTGCACTAAG CCTTACAATA CCTCTGTCCA 1381 TAATAGCTGA CATGTGTATG CAAAAGGTGC AGTTTTCTTG GTTATTTTTT GCAGGAGCTA 1441 TCCCTGTATT TTTTTCATTT TTTATTGTAA CTCTCCTATG CCATTATAAT AATTGGGATC 1501 CTGTGATGGT GGGAATCAGA AGAATATTTG CTTTTATATG CAGAAAACAT CGAATTCAGA 1561 GAGTTCCAGA AGACAGCGAA CAGTGTGAGA GTCTCATTTC TATGCACAGT GTTTCTCAGG 1621 AGGATGGCGC TAGTTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1681 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1741 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA TAGCAGGATT GGTCCTCGGC 1801 ACAAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt