Construct: ORF TRCN0000470948
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013906.1_s317c1
- Derived from:
- ccsbBroadEn_10805
- DNA Barcode:
- TATCCTAGATGCTGATGGGATATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EPHB6 (2051)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470948
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2051 | EPHB6 | EPH receptor B6 | NM_001280794.2 | 99.8% | 99.8% | 94G>T;894A>G;1011A>G |
| 2 | human | 2051 | EPHB6 | EPH receptor B6 | NM_001280795.2 | 99.8% | 99.8% | 94G>T;894A>G;1011A>G |
| 3 | human | 2051 | EPHB6 | EPH receptor B6 | NM_004445.6 | 71.2% | 71.2% | (many diffs) |
| 4 | human | 2051 | EPHB6 | EPH receptor B6 | XM_011515879.1 | 71.2% | 71.2% | (many diffs) |
| 5 | human | 2051 | EPHB6 | EPH receptor B6 | XM_011515880.1 | 71.2% | 71.2% | (many diffs) |
| 6 | human | 2051 | EPHB6 | EPH receptor B6 | XM_024446674.1 | 71.2% | 71.2% | (many diffs) |
| 7 | human | 2051 | EPHB6 | EPH receptor B6 | XM_011515881.3 | 71.1% | 71.1% | (many diffs) |
| 8 | human | 2051 | EPHB6 | EPH receptor B6 | XM_011515882.2 | 68.1% | 68.1% | (many diffs) |
| 9 | human | 2051 | EPHB6 | EPH receptor B6 | XM_024446675.1 | 68.1% | 68.1% | (many diffs) |
| 10 | human | 2051 | EPHB6 | EPH receptor B6 | NR_104001.2 | 65.2% | (many diffs) | |
| 11 | mouse | 13848 | Ephb6 | Eph receptor B6 | NM_001146351.1 | 62.9% | 66.6% | (many diffs) |
| 12 | mouse | 13848 | Ephb6 | Eph receptor B6 | NM_007680.4 | 62.9% | 66.6% | (many diffs) |
| 13 | mouse | 13848 | Ephb6 | Eph receptor B6 | XR_377419.3 | 22.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2256
- ORF length:
- 2187
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggtagctgtc gggggctgcc gctgccagcc tggataccaa ccagcacgag 121 gagacaaggc ctgccaagcc tgcccacggg ggctctataa gtcttctgct gggaatgctc 181 cctgctcacc atgccctgcc cgcagtcacg ctcccaaccc agcagccccc gtttgcccct 241 gcctggaggg cttctaccgg gccagttccg acccaccaga ggccccctgc actggtcctc 301 catcggctcc ccaggagctt tggtttgagg tgcaaggctc agcactcatg ctacactggc 361 gcctgcctcg ggagctgggg ggtcgagggg acctgctctt caatgtcgtg tgcaaggagt 421 gtgaaggccg ccaggaacct gccagcggtg gtgggggcac ttgtcaccgc tgcagggatg 481 aggtccactt cgaccctcgc cagagaggcc tgactgagag ccgagtgtta gtggggggac 541 tccgggcaca cgtaccctac atcttagagg tgcaggctgt taatggggtg tctgagctca 601 gccctgaccc tcctcaggct gcagccatca atgtcagcac cagccatgaa gtgccctctg 661 ctgtccctgt ggtgcaccag gtgagccggg catccaacag catcacggtg tcctggccgc 721 agcccgacca gaccaatggg aacatcctgg actatcagct ccgctactat gaccaggcag 781 aagacgaatc ccactccttc accctgacca gcgagaccaa cactgccacc gtgacacagc 841 tgagccctgg ccacatctat ggtttccagg tgcgggcccg gactgctgcc ggccacggcc 901 cctacggggg caaagtctat ttccagacac ttcctcaagg ggagctgtct tcccagcttc 961 cggaaagact ctccttggtg atcggctcca tcctgggggc tttggccttc ctcctgctgg 1021 cagccatcac cgtgctggcg gtcgtcttcc agcggaagcg gcgtgggact ggctacacgg 1081 agcagctgca gcaatacagc agcccaggac tcggggtgaa gtattacatc gacccctcca 1141 cctacgagga cccctgtcag gccatccgag aacttgcccg ggaagtcgat cctgcttata 1201 tcaagattga ggaggtcatt gggacaggct cttttggaga agtgcgccag ggccgcctgc 1261 agccacgggg acggagggag cagactgtgg ccatccaggc cctgtgggcc gggggcgccg 1321 aaagcctgca gatgaccttc ctgggccggg ccgcagtgct gggtcagttc cagcacccca 1381 acatcctgcg gctggagggc gtggtcacca agagccgacc cctcatggtg ctgacggagt 1441 tcatggagct tggccccctg gacagcttcc tcaggcagcg ggagggccag ttcagcagcc 1501 tgcagctggt ggccatgcag cggggagtgg ctgctgccat gcagtacctg tccagctttg 1561 ccttcgtcca tcgctcgctg tctgcccaca gcgtgctggt gaatagccac ttggtgtgca 1621 aggtggcccg tcttggccac agtcctcagg gcccaagttg tttgcttcgc tgggcagccc 1681 cagaggtcat tgcacatgga aagcatacaa catccagtga tgtctggagc tttgggatac 1741 tcatgtggga agtgatgagt tatggagaac ggccttactg ggacatgagt gagcaggagg 1801 tactaaatgc aatagagcag gagttccggc tgcccccgcc tccaggctgt ccTCCTGGAT 1861 TACATCTACT TATGTTGGAC ACTTGGCAGA AGGACCGTGC CCGGCGGCCT CATTTTGACC 1921 AGCTGGTGGC TGCATTTGAC AAGATGATCC GCAAGCCAGA TACCCTGCAG GCTGGCGGGG 1981 ACCCAGGGGA AAGGCCTTCC CAGGCCCTTC TGACCCCTGT GGCCCTGGAC TTTCCTTGTC 2041 TGGACTCACC CCAGGCCTGG CTTTCAGCCA TTGGACTGGA GTGCTACCAG GACAACTTCT 2101 CCAAGTTTGG CCTCTGTACC TTCAGTGATG TGGCTCAGCT CAGCCTAGAA GACCTGCCTG 2161 CCCTGGGCAT CACCCTGGCT GGCCACCAGA AGAAGCTGCT GCACCACATC CAGCTCCTTC 2221 AGCAACACCT GAGGCAGCAG GGCTCAGTGG AGGTCTTGCC AACTTTCTTG TACAAAGTGG 2281 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 2341 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 2401 ATATCCTAGA TGCTGATGGG ATATCACGCG TTAAGTCgac aatcaacctc tggattacaa 2461 aatttgtgaa agatt