Transcript: Mouse NM_001146351.1

Mus musculus Eph receptor B6 (Ephb6), transcript variant 1, mRNA.

Source:
NCBI, updated 2017-05-21
Taxon:
Mus musculus (mouse)
Gene:
Ephb6 (13848)
Length:
3760
CDS:
513..3557

Additional Resources:

NCBI RefSeq record:
NM_001146351.1
NBCI Gene record:
Ephb6 (13848)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001146351.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000023646 CCCGAAGTGTGCTAGTGAATA pLKO.1 2884 CDS 100% 13.200 18.480 N Ephb6 n/a
2 TRCN0000321894 CCCGAAGTGTGCTAGTGAATA pLKO_005 2884 CDS 100% 13.200 18.480 N Ephb6 n/a
3 TRCN0000023644 CGAGAGGTTGACCCTACATAT pLKO.1 2478 CDS 100% 13.200 18.480 N Ephb6 n/a
4 TRCN0000321893 CGAGAGGTTGACCCTACATAT pLKO_005 2478 CDS 100% 13.200 18.480 N Ephb6 n/a
5 TRCN0000023645 CCACAGCCTGAGCAAACAAAT pLKO.1 2016 CDS 100% 13.200 9.240 N Ephb6 n/a
6 TRCN0000321892 CCACAGCCTGAGCAAACAAAT pLKO_005 2016 CDS 100% 13.200 9.240 N Ephb6 n/a
7 TRCN0000321896 GAACGAGCAGGAGGTACTAAA pLKO_005 3086 CDS 100% 13.200 9.240 N Ephb6 n/a
8 TRCN0000023647 TGGACCAAAGTGGACACAATT pLKO.1 969 CDS 100% 13.200 9.240 N Ephb6 n/a
9 TRCN0000023648 CCCTGGACTACATCTACTGAT pLKO.1 3152 CDS 100% 4.950 3.465 N Ephb6 n/a
10 TRCN0000321895 CCCTGGACTACATCTACTGAT pLKO_005 3152 CDS 100% 4.950 3.465 N Ephb6 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001146351.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14630 pDONR223 0% 86.4% 91.9% None (many diffs) n/a
2 ccsbBroad304_14630 pLX_304 0% 86.4% 91.9% V5 (many diffs) n/a
3 TRCN0000481066 ACATACGAGACCCATCCACGAAGA pLX_317 11.6% 86.4% 91.9% V5 (many diffs) n/a
4 ccsbBroadEn_10805 pDONR223 100% 62.9% 66.6% None (many diffs) n/a
5 ccsbBroad304_10805 pLX_304 0% 62.9% 66.6% V5 (many diffs) n/a
6 TRCN0000470948 TATCCTAGATGCTGATGGGATATC pLX_317 18.7% 62.9% 66.6% V5 (many diffs) n/a
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