Construct: ORF TRCN0000470949
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003629.1_s317c1
- Derived from:
- ccsbBroadEn_01583
- DNA Barcode:
- CAAAATCACACAATAGCTGTAACT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SP4 (6671)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470949
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6671 | SP4 | Sp4 transcription factor | NM_003112.5 | 100% | 100% | |
| 2 | human | 6671 | SP4 | Sp4 transcription factor | NM_001326542.2 | 97.8% | 97.8% | 0_1ins51 |
| 3 | human | 6671 | SP4 | Sp4 transcription factor | XM_011515486.2 | 90.6% | 90.1% | (many diffs) |
| 4 | human | 6671 | SP4 | Sp4 transcription factor | XM_005249829.4 | 89.8% | 89.5% | (many diffs) |
| 5 | human | 6671 | SP4 | Sp4 transcription factor | XM_017012557.1 | 83% | 80.7% | (many diffs) |
| 6 | human | 6671 | SP4 | Sp4 transcription factor | XM_011515487.2 | 72.5% | 71.1% | (many diffs) |
| 7 | human | 6671 | SP4 | Sp4 transcription factor | NM_001326543.2 | 60% | 60% | 0_1ins939 |
| 8 | mouse | 20688 | Sp4 | trans-acting transcription ... | NM_009239.4 | 92.6% | 97.4% | (many diffs) |
| 9 | mouse | 20688 | Sp4 | trans-acting transcription ... | NM_001166385.2 | 92.4% | 97.1% | (many diffs) |
| 10 | mouse | 20688 | Sp4 | trans-acting transcription ... | XM_017315007.1 | 90.6% | 95.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2421
- ORF length:
- 2352
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gagcgatcag aagaaggagg aggaggagga ggcggcagcg gcagcggcga 121 tggctacaga aggagggaaa acctctgagc cagagaataa caataaaaaa cccaaaacct 181 caggctccca ggactctcag ccctctcctc tggctttact ggcagctact tgcagcaaaa 241 tagggactcc tggtgaaaat caagcaactg gacaacaaca aattattata gatccaagtc 301 aaggattggt gcaacttcaa aatcaaccac aacagctaga actggtaaca acgcaacttg 361 ctggaaacgc ttggcaactt gttgcctcca ctcctcctgc ttcaaaagag aataacgttt 421 ctcaaccagc ctctagttcg tctagttctt ccagcagtaa taacgggagt gcatctccta 481 caaaaactaa atcaggtaat tcttccaccc ctggtcaatt tcaagtcata caagtacaaa 541 atccaagtgg tagtgtacag taccaagtaa ttccacaact tcagacagtg gaaggtcaac 601 aaattcaaat caatccaact agtagttcat ctctacagga tttgcagggt caaattcagc 661 tcatttctgc aggtaataat caagctatac tcacagctgc taacaggaca gcttctggga 721 atattcttgc tcaaaacctg gcaaatcaga cagttccggt ccaaattaga cctggtgttt 781 caataccact gcagttacag actcttcctg gtactcaggc tcaagttgta acaaccctac 841 caattaacat tggaggagtg actctagctt tgccagtgat aaacaacgtg gctgccggag 901 gagggactgg gcaggttggc cagcctgctg ctactgctga tagtgggact tccaatggga 961 atcaattagt ttccacaccc accaacacca ctacttctgc cagtactatg ccagaatctc 1021 cctcctcctc cactacctgc acaaccactg cttcaacgtc tttgacaagc agtgacacat 1081 tagtgagctc agcagatact ggccagtatg caagcacatc agccagtagt tctgaacgca 1141 ccattgaaga atctcaaaca cctgctgcta ctgagtctga agcccagagc tccagtcagc 1201 ttcagcctaa tggaatgcag aatgcacagg atcaatcaaa ttctcttcag caggtgcaaa 1261 ttgtaggcca acctatctta cagcagatcc agatccaaca gcctcagcaa cagatcattc 1321 aggctattcc accacagtcg tttcaactcc agtcagggca gacgattcag accatccagc 1381 agcagccttt acagaatgtt caacttcaag cagtaaatcc gactcaggtg cttatcaggg 1441 ctccaacttt aacaccttca gggcaaatca gttggcaaac tgtacaggtt cagaatattc 1501 agagtctttc aaatttgcaa gttcagaatg ctgggttatc ccaacaatta accatcaccc 1561 cagtgtcttc aagtggtggc acaactcttg ctcagattgc tcctgtggct gttgctggtg 1621 ccccaataac tttgaatact gcccagcttg catcagtgcc taaccttcag acagtgagcg 1681 ttgccaacct gggtgctgca ggtgttcaag tgcagggagt tcccgttaca atcactagtg 1741 ttgcaggtca gcagcaagga caagatggag taaaagtcca gcaagctact atagctcctg 1801 taactgtagc agttggagga attgctaatg ccacgatagg tgctgttagt cctgaccaac 1861 tcacacaagt gcatttgcag caaggccagc agacttctga tcaagaggta caacctggca 1921 agaggcttcG AAGAGTTGCC TGTTCCTGTC CTAATTGTAG GGAAGGAGAA GGAAGAGGCA 1981 GTAATGAACC AGGAAAAAAG AAGCAGCATA TCTGTCATAT TGAAGGATGT GGTAAAGTTT 2041 ATGGCAAAAC ATCTCATTTA CGAGCACATC TTCGCTGGCA TACTGGAGAA AGACCTTTTA 2101 TATGCAACTG GATGTTTTGT GGCAAAAGAT TCACACGGAG TGATGAGCTC CAGAGACATA 2161 GAAGAACCCA TACAGGTGAA AAGAGATTTG AATGCCCGGA ATGTTCTAAA AGGTTTATGC 2221 GGAGTGATCA TCTCTCCAAA CATGTCAAAA CGCACCAGAA TAAAAAAGGT GGTGGGACAG 2281 CTCTTGCCAT TGTTACCTCG GGAGAACTGG ACTCATCTGT TACAGAGGTG CTTGGCTCCC 2341 CAAGAATTGT CACAGTTGCA GCCATTTCTC AAGATTCGAA TCCAGCAACT CCCAATGTTT 2401 CAACCAACAT GGAAGAATTC TTGCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 2461 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 2521 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACAAA ATCACACAAT 2581 AGCTGTAACT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt