Transcript: Human XM_005249829.4

PREDICTED: Homo sapiens Sp4 transcription factor (SP4), transcript variant X2, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
SP4 (6671)
Length:
7387
CDS:
153..2273

Additional Resources:

NCBI RefSeq record:
XM_005249829.4
NBCI Gene record:
SP4 (6671)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_005249829.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000311011 TGTAACAACCCTACCAATTAA pLKO_005 911 CDS 100% 15.000 21.000 N Sp4 n/a
2 TRCN0000425976 TGTAACAACCCTACCAATTAA pLKO_005 911 CDS 100% 15.000 21.000 N SP4 n/a
3 TRCN0000421768 CAGACAGTTCCGGTCCAAATT pLKO_005 831 CDS 100% 13.200 18.480 N SP4 n/a
4 TRCN0000436110 GTTCAACTTCAAGCAGTAAAT pLKO_005 1482 CDS 100% 13.200 18.480 N SP4 n/a
5 TRCN0000419469 ACCACTGCTTCAACGTCTTTG pLKO_005 1128 CDS 100% 10.800 15.120 N SP4 n/a
6 TRCN0000020511 CCCGTTACAATCACTAGTGTT pLKO.1 1806 CDS 100% 0.000 0.000 N SP4 n/a
7 TRCN0000435080 CTTATCAGGGCTCCAACTTTA pLKO_005 1515 CDS 100% 13.200 10.560 N SP4 n/a
8 TRCN0000412698 TGCCTGTTCCTGTCCTAATTG pLKO_005 2021 CDS 100% 13.200 10.560 N SP4 n/a
9 TRCN0000426367 ACTACTTCTGCCAGTACTATG pLKO_005 1074 CDS 100% 10.800 7.560 N SP4 n/a
10 TRCN0000020510 GCAGGTAATAATCAAGCTATA pLKO.1 753 CDS 100% 10.800 7.560 N SP4 n/a
11 TRCN0000020512 GCAACAGATCATTCAGGCTAT pLKO.1 1391 CDS 100% 4.050 2.835 N SP4 n/a
12 TRCN0000020513 GCAGTAATAACGGGAGTGCAT pLKO.1 538 CDS 100% 2.640 1.848 N SP4 n/a
13 TRCN0000086294 CCAGCAGTAATAACGGGAGTT pLKO.1 535 CDS 100% 4.050 2.835 N Sp4 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_005249829.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01583 pDONR223 100% 89.8% 89.5% None (many diffs) n/a
2 ccsbBroad304_01583 pLX_304 0% 89.8% 89.5% V5 (many diffs) n/a
3 TRCN0000470949 CAAAATCACACAATAGCTGTAACT pLX_317 21.1% 89.8% 89.5% V5 (many diffs) n/a
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