Transcript: Human NM_001326543.2

Homo sapiens Sp4 transcription factor (SP4), transcript variant 3, mRNA.

Source:
NCBI, updated 2019-09-28
Taxon:
Homo sapiens (human)
Gene:
SP4 (6671)
Length:
6043
CDS:
1075..2490

Additional Resources:

NCBI RefSeq record:
NM_001326543.2
NBCI Gene record:
SP4 (6671)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001326543.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000311011 TGTAACAACCCTACCAATTAA pLKO_005 894 5UTR 100% 15.000 21.000 N Sp4 n/a
2 TRCN0000425976 TGTAACAACCCTACCAATTAA pLKO_005 894 5UTR 100% 15.000 21.000 N SP4 n/a
3 TRCN0000421768 CAGACAGTTCCGGTCCAAATT pLKO_005 814 5UTR 100% 13.200 18.480 N SP4 n/a
4 TRCN0000436110 GTTCAACTTCAAGCAGTAAAT pLKO_005 1465 CDS 100% 13.200 18.480 N SP4 n/a
5 TRCN0000419469 ACCACTGCTTCAACGTCTTTG pLKO_005 1111 CDS 100% 10.800 15.120 N SP4 n/a
6 TRCN0000020511 CCCGTTACAATCACTAGTGTT pLKO.1 1789 CDS 100% 0.000 0.000 N SP4 n/a
7 TRCN0000435080 CTTATCAGGGCTCCAACTTTA pLKO_005 1498 CDS 100% 13.200 10.560 N SP4 n/a
8 TRCN0000412698 TGCCTGTTCCTGTCCTAATTG pLKO_005 2004 CDS 100% 13.200 10.560 N SP4 n/a
9 TRCN0000431990 GAATCTTCCCATCTCTTAATA pLKO_005 2757 3UTR 100% 15.000 10.500 N SP4 n/a
10 TRCN0000423687 GTATTTCCTCCATACTATAAG pLKO_005 2697 3UTR 100% 13.200 9.240 N SP4 n/a
11 TRCN0000426367 ACTACTTCTGCCAGTACTATG pLKO_005 1057 5UTR 100% 10.800 7.560 N SP4 n/a
12 TRCN0000020510 GCAGGTAATAATCAAGCTATA pLKO.1 736 5UTR 100% 10.800 7.560 N SP4 n/a
13 TRCN0000020509 CCTCTAGTGCTGCACTTGTTT pLKO.1 2511 3UTR 100% 5.625 3.938 N SP4 n/a
14 TRCN0000020512 GCAACAGATCATTCAGGCTAT pLKO.1 1374 CDS 100% 4.050 2.835 N SP4 n/a
15 TRCN0000020513 GCAGTAATAACGGGAGTGCAT pLKO.1 521 5UTR 100% 2.640 1.848 N SP4 n/a
16 TRCN0000430981 GCCACCATGCCTGGCTAATTT pLKO_005 3933 3UTR 100% 15.000 7.500 Y GTF2IRD2 n/a
17 TRCN0000086294 CCAGCAGTAATAACGGGAGTT pLKO.1 518 5UTR 100% 4.050 2.835 N Sp4 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001326543.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01583 pDONR223 100% 60% 60% None 0_1ins939 n/a
2 ccsbBroad304_01583 pLX_304 0% 60% 60% V5 0_1ins939 n/a
3 TRCN0000470949 CAAAATCACACAATAGCTGTAACT pLX_317 21.1% 60% 60% V5 0_1ins939 n/a
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