Construct: ORF TRCN0000470969
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014293.1_s317c1
- Derived from:
- ccsbBroadEn_08177
- DNA Barcode:
- AGCATCCGTTTTTAGTTGCCCGCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CHST11 (50515)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470969
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 50515 | CHST11 | carbohydrate sulfotransfera... | NM_018413.6 | 99.9% | 100% | 1038A>C |
| 2 | human | 50515 | CHST11 | carbohydrate sulfotransfera... | NM_001173982.2 | 98.4% | 98.2% | 103_104insGTATGTTGCACCCAG;1023A>C |
| 3 | human | 50515 | CHST11 | carbohydrate sulfotransfera... | XM_017019369.1 | 86.3% | 76.2% | (many diffs) |
| 4 | mouse | 58250 | Chst11 | carbohydrate sulfotransfera... | NM_021439.2 | 91% | 96.8% | (many diffs) |
| 5 | mouse | 58250 | Chst11 | carbohydrate sulfotransfera... | XM_006513909.3 | 89.5% | 95.1% | (many diffs) |
| 6 | mouse | 58250 | Chst11 | carbohydrate sulfotransfera... | XM_006513908.3 | 81.8% | 85.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1122
- ORF length:
- 1056
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gccagcgctg ctggaagtga tgaggatgaa cagaatctgc cggatggtgc 121 tggccacttg cttgggatcc tttatcctgg tcatcttcta tttccaaagt atgttgcacc 181 cagtcatgcg gaggaatccc tttggtgtgg acatctgctg ccggaagggg tcccgaagcc 241 ccctgcagga actctacaac ccaatccagc tggagctctc aaacactgct gtcctgcacc 301 agatgcggcg ggaccaggtg acagacacgt gccgagccaa cagcgccaca agccgtaagc 361 ggagggtgct gacccccaac gacctgaagc acttggtggt ggatgaggac cacgagctca 421 tctactgcta cgtgcccaag gtggcctgca ccaactggaa gcggctcatg atggtcctga 481 ccgggcgggg gaagtacagc gaccccatgg agatcccggc caacgaggca cacgtctccg 541 ccaacctgaa gaccctgaac cagtacagca tcccagaaat caaccaccgc ttgaaaagct 601 acatgaagtt cctgtttgtc cgggagccct tcgagaggct agtgtccgcc taccgcaaca 661 agttcaccca gaagtacaac atctccttcc acaagcggta cggcaccaag atcatcaaac 721 gccagcggaa gaacgccacc caggaggccc tgcgcaaagg GGACGATGTC AAATTCGAGG 781 AGTTTGTGGC CTATCTCATC GACCCACACA CCCAGCGGGA GGAGCCTTTC AACGAACACT 841 GGCAAACCGT CTACTCACTC TGCCATCCCT GCCACATCCA CTATGACCTC GTGGGCAAGT 901 ACGAGACACT GGAAGAGGAT TCTAATTACG TCCTGCAGCT GGCAGGAGTG GGCAGCTACC 961 TGAAGTTCCC CACCTATGCA AAGTCTACGA GAACTACTGA TGAAATGACC ACAGAATTCT 1021 TCCAGAACAT CAGCTCAGAG CACCAAACGC AGCTGTACGA AGTCTACAAA CTCGATTTTT 1081 TAATGTTCAA TTACTCAGTG CCCAGCTACC TGAAATTGGA ATACCCAACT TTCTTGTACA 1141 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1201 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1261 AGGACGAAGC ATCCGTTTTT AGTTGCCCGC TACGCGTTAA GTCgacaatc aacctctgga 1321 ttacaaaatt tgtgaaagat t