Construct: ORF TRCN0000470986
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018862.3_s317c1
- Derived from:
- ccsbBroadEn_14935
- DNA Barcode:
- GCGGATCGTTTCACATTGGTTTGT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- MAPKAPK2 (9261)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470986
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9261 | MAPKAPK2 | MAPK activated protein kina... | NM_032960.4 | 95.5% | 34% | (many diffs) |
| 2 | human | 9261 | MAPKAPK2 | MAPK activated protein kina... | NM_004759.5 | 85.8% | 36.7% | (many diffs) |
| 3 | human | 9261 | MAPKAPK2 | MAPK activated protein kina... | XM_005273353.3 | 85.8% | 36.6% | (many diffs) |
| 4 | human | 9261 | MAPKAPK2 | MAPK activated protein kina... | XM_017002810.1 | 79.9% | 15.6% | (many diffs) |
| 5 | mouse | 17164 | Mapkapk2 | MAP kinase-activated protei... | NM_008551.2 | 87.7% | 31.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 519
- ORF length:
- 450
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gctgtccaac tcccagggcc agagcccgcc ggtgccgttc cccgccccgg 121 ccccgccgcc gcccccgcag cagttcccgc agttccacgt caagtccggc ctgcagatca 181 agaagaacgc catcatcgat gactacaagg tcaccagcca ggtcctgggg ctgggcatca 241 acggcaaagt tttgcagatc ttcaacaaga ggacccagga gaaattcgcc ctcaaaatgc 301 ttcaggactg ccccaaggcc cgcagggagg tggagctgca ctggcgggcc tcccagtgcc 361 cgcacatcgt acggatcgtg gatgtgtacg agaatctgta cgcagggagg aagtgcctgc 421 tgattgtcat ggaatgtttg gacggtggag aactctttag ccgaatccca ggatcgaggg 481 agaccaggca ttcacagaaa gagaagcatc cgaaatcatg aagagcatcg gtgaggccat 541 ccagtatctg cattcaatca acattgccca tcgggatgtc aagcctgaga atctcttata 601 cacctccaaa aggcccaacg tcatcctgaa actcactgac tttggctttg ccaaggaaac 661 caccagccac aactctttga ccactccttg ttatacaccg tactatgtgg ctccagaagt 721 gctgggtcca gagaagtatg acaagtcctg tgacatgtgg tccctgggtg tcatcatgta 781 catcctgctg tgtgggtatc cccccTTCTA CTCCAACCAC GGCCTTGCCA TCTCTCCGGG 841 CATGAAGACT CGCATCCGAA TGGGCCAGTA TGAATTTCCC AACCCAGAAT GGTCAGAAGT 901 ATCAGAGGAA GTGAAGATGC TCATTCGGAA TCTGCTGAAA ACAGAGCCCA CCCAGAGAAT 961 GACCATCACC GAGTTTATGA ACCACCCTTG GATCATGCAA TCAACAAAGG TCCCTCAAAC 1021 CCCACTGCAC ACCAGCCGGG TCCTGAAGGA GGACAAGGAG CGGTGGGAGG ATGTCAAGGA 1081 GGAGATGACC AGTGCCTTGG CCACAATGCG CGTTGACTAC GAGCAGATCA AGATAAAAAA 1141 GATTGAAGAT GCATCCAACC CTCTGCTGCT GAAGAGGCGG AAGAAAGCTC GGGCCCTGGA 1201 GGCTGCGGCT CTGGCCCACT TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC 1261 TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA 1321 AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGCGGA TCGTTTCACA 1381 TTGGTTTGTA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg tgaaagatt