Construct: ORF TRCN0000470986

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018862.3_s317c1
Derived from:
ccsbBroadEn_14935
DNA Barcode:
GCGGATCGTTTCACATTGGTTTGT
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
MAPKAPK2 (9261)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000470986
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9261 MAPKAPK2 MAPK activated protein kina... NM_032960.4 95.5% 34% (many diffs)
2 human 9261 MAPKAPK2 MAPK activated protein kina... NM_004759.5 85.8% 36.7% (many diffs)
3 human 9261 MAPKAPK2 MAPK activated protein kina... XM_005273353.3 85.8% 36.6% (many diffs)
4 human 9261 MAPKAPK2 MAPK activated protein kina... XM_017002810.1 79.9% 15.6% (many diffs)
5 mouse 17164 Mapkapk2 MAP kinase-activated protei... NM_008551.2 87.7% 31.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
519
ORF length:
450
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gctgtccaac tcccagggcc agagcccgcc ggtgccgttc cccgccccgg
121ccccgccgcc gcccccgcag cagttcccgc agttccacgt caagtccggc ctgcagatca
181agaagaacgc catcatcgat gactacaagg tcaccagcca ggtcctgggg ctgggcatca
241acggcaaagt tttgcagatc ttcaacaaga ggacccagga gaaattcgcc ctcaaaatgc
301ttcaggactg ccccaaggcc cgcagggagg tggagctgca ctggcgggcc tcccagtgcc
361cgcacatcgt acggatcgtg gatgtgtacg agaatctgta cgcagggagg aagtgcctgc
421tgattgtcat ggaatgtttg gacggtggag aactctttag ccgaatccca ggatcgaggg
481agaccaggca ttcacagaaa gagaagcatc cgaaatcatg aagagcatcg gtgaggccat
541ccagtatctg cattcaatca acattgccca tcgggatgtc aagcctgaga atctcttata
601cacctccaaa aggcccaacg tcatcctgaa actcactgac tttggctttg ccaaggaaac
661caccagccac aactctttga ccactccttg ttatacaccg tactatgtgg ctccagaagt
721gctgggtcca gagaagtatg acaagtcctg tgacatgtgg tccctgggtg tcatcatgta
781catcctgctg tgtgggtatc cccccTTCTA CTCCAACCAC GGCCTTGCCA TCTCTCCGGG
841CATGAAGACT CGCATCCGAA TGGGCCAGTA TGAATTTCCC AACCCAGAAT GGTCAGAAGT
901ATCAGAGGAA GTGAAGATGC TCATTCGGAA TCTGCTGAAA ACAGAGCCCA CCCAGAGAAT
961GACCATCACC GAGTTTATGA ACCACCCTTG GATCATGCAA TCAACAAAGG TCCCTCAAAC
1021CCCACTGCAC ACCAGCCGGG TCCTGAAGGA GGACAAGGAG CGGTGGGAGG ATGTCAAGGA
1081GGAGATGACC AGTGCCTTGG CCACAATGCG CGTTGACTAC GAGCAGATCA AGATAAAAAA
1141GATTGAAGAT GCATCCAACC CTCTGCTGCT GAAGAGGCGG AAGAAAGCTC GGGCCCTGGA
1201GGCTGCGGCT CTGGCCCACT TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC
1261TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA
1321AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGCGGA TCGTTTCACA
1381TTGGTTTGTA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg tgaaagatt

Download FASTA (ORF) (Full)