Construct: ORF TRCN0000471008
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004708.1_s317c1
- Derived from:
- ccsbBroadEn_06291
- DNA Barcode:
- GATTTGGTAGTCACACGCTTACGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GNAI1 (2770)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471008
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2770 | GNAI1 | G protein subunit alpha i1 | NM_002069.6 | 99.7% | 99.4% | 655A>G;819C>T;863C>A |
| 2 | human | 2770 | GNAI1 | G protein subunit alpha i1 | NM_001256414.2 | 85% | 84.7% | (many diffs) |
| 3 | human | 2773 | GNAI3 | G protein subunit alpha i3 | NM_006496.4 | 80.9% | 93.2% | (many diffs) |
| 4 | mouse | 14677 | Gnai1 | guanine nucleotide binding ... | NM_010305.1 | 88.2% | 99.4% | (many diffs) |
| 5 | mouse | 14679 | Gnai3 | guanine nucleotide binding ... | NM_010306.3 | 80.4% | 93.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1128
- ORF length:
- 1062
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg ctgcacgctg agcgccgagg acaaggcggc ggtggagcgg agtaagatga 121 tcgaccgcaa cctccgtgag gacggcgaga aggcggcgcg cgaggtcaag ctgctgctgc 181 tcggtgctgg tgaatctggt aaaagtacaa ttgtgaagca gatgaaaatt atccatgaag 241 ctggttattc agaagaggag tgtaaacaat acaaagcagt ggtctacagt aacaccatcc 301 agtcaattat tgctatcatt agggctatgg ggaggttgaa gatagacttt ggtgactcag 361 cccgggcgga tgatgcacgc caactctttg tgctagctgg agctgctgaa gaaggcttta 421 tgactgcaga acttgctgga gttataaaga gattgtggaa agatagtggt gtacaagcct 481 gtttcaacag atcccgagag taccagctta atgattctgc agcatactat ttgaatgact 541 tggacagaat agctcaacca aattacatcc cgactcaaca agatgttctc agaactagag 601 tgaaaactac aggaattgtt gaaacccatt ttactttcaa agatcttcat tttaaaatgt 661 ttgatgtggg aggtcagaga tctgagcgga agaagtggat tcattgcttc gaaggagtgg 721 cggcgaTCAT CTTCTGTGTA GCACTGAGTG ACTACGACCT GGTTCTAGCT GAAGATGAAG 781 AAATGAACCG AATGCATGAA AGCATGAAAT TGTTTGACAG CATATGTAAC AACAAGTGGT 841 TTACAGATAC ATCCATTATA CTTTTTCTAA ACAAGAAGGA TCTTTTTGAA GAAAAAATCA 901 AAAAGAGCCC TCTCACTATA TGCTATCAAG AATATGCAGG ATCAAACACA TATGAAGAGG 961 CAGCTGCATA TATTCAATGT CAGTTTGAAG ACCTCAATAA AAGAAAGGAC ACAAAGGAAA 1021 TATACACCCA CTTCACATGT GCCACAGATA CTAAGAATGT GCAGTTTGTT TTTGATGCTG 1081 TAACAGATGT CATCATAAAA AATAATCTAA AAGATTGTGG TCTCTTTTAC CCAACTTTCT 1141 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1201 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1261 GTGGAAAGGA CGAGATTTGG TAGTCACACG CTTACGTACG CGTTAAGTCg acaatcaacc 1321 tctggattac aaaatttgtg aaagatt