Transcript: Human NM_001002273.2

Homo sapiens Fc fragment of IgG receptor IIb (FCGR2B), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-09-01
Taxon:
Homo sapiens (human)
Gene:
FCGR2B (2213)
Length:
2114
CDS:
128..1000

Additional Resources:

NCBI RefSeq record:
NM_001002273.2
NBCI Gene record:
FCGR2B (2213)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001002273.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000029585 CCTGATGACCAGAACCGTATT pLKO.1 977 CDS 100% 10.800 15.120 N FCGR2C n/a
2 TRCN0000434820 ACCGTATTTAGTCTCCATTGT pLKO_005 990 CDS 100% 4.950 6.930 N FCGR2B n/a
3 TRCN0000029579 TGATGACCAGAACCGTATTTA pLKO.1 979 CDS 100% 15.000 12.000 N FCGR2B n/a
4 TRCN0000416201 CACAATCACCTATTCACTTCT pLKO_005 931 CDS 100% 4.950 3.465 N FCGR2B n/a
5 TRCN0000029583 GCCAATCCCACTAATCCTGAT pLKO.1 884 CDS 100% 4.050 2.835 N FCGR2B n/a
6 TRCN0000433670 ACGTGAGAACAATCATGTAAA pLKO_005 1328 3UTR 100% 13.200 7.920 N FCGR2B n/a
7 TRCN0000029586 GCTGAGAACACAATCACCTAT pLKO.1 923 CDS 100% 4.950 2.970 N FCGR2C n/a
8 TRCN0000029581 CCACAGTCACAGTGGTGATTA pLKO.1 685 CDS 100% 13.200 6.600 Y FCGR2B n/a
9 TRCN0000029584 GCCTTGATCTACTGCAGGAAA pLKO.1 851 CDS 100% 4.950 2.475 Y FCGR2C n/a
10 TRCN0000029587 CAGTGGTTCCACAATGGGAAT pLKO.1 371 CDS 100% 4.050 2.025 Y FCGR2C n/a
11 TRCN0000029582 CATATGCTTCTGTGGACAGCT pLKO.1 206 CDS 100% 0.000 0.000 Y FCGR2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001002273.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00546 pDONR223 100% 93.5% 93.5% None 132_133insGCA;754_755ins57 n/a
2 ccsbBroad304_00546 pLX_304 0% 93.5% 93.5% V5 132_133insGCA;754_755ins57 n/a
3 TRCN0000471042 AAGTCCACGAAAGCGTGGAATATT pLX_317 42.2% 93.5% 93.5% V5 132_133insGCA;754_755ins57 n/a
4 ccsbBroadEn_06198 pDONR223 100% 75% 60.9% None (many diffs) n/a
5 ccsbBroad304_06198 pLX_304 0% 75% 60.9% V5 (many diffs) n/a
6 TRCN0000472330 ACTGGGGCTCGAGCAGACAGCCGC pLX_317 46.5% 75% 60.9% V5 (many diffs) n/a
7 ccsbBroadEn_06199 pDONR223 100% 74.8% 60.3% None (many diffs) n/a
8 ccsbBroad304_06199 pLX_304 0% 74.8% 60.3% V5 (many diffs) n/a
9 TRCN0000475216 TGCCCATTGCGGCCGTTTAATATC pLX_317 25% 74.8% 60.3% V5 (many diffs) n/a
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