Construct: ORF TRCN0000471048
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002035.1_s317c1
- Derived from:
- ccsbBroadEn_08111
- DNA Barcode:
- TCACCACTGCGTTAATACACGGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CNIH4 (29097)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471048
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 29097 | CNIH4 | cornichon family AMPA recep... | NM_014184.4 | 99.7% | 99.2% | 14T>A |
| 2 | human | 29097 | CNIH4 | cornichon family AMPA recep... | NM_001277200.2 | 95% | 93.5% | (many diffs) |
| 3 | human | 29097 | CNIH4 | cornichon family AMPA recep... | NM_001277198.2 | 81.7% | 62.5% | 14T>A;250_251ins47;342_343ins28 |
| 4 | human | 29097 | CNIH4 | cornichon family AMPA recep... | NM_001277197.2 | 65.9% | 65.4% | 14T>A;250_251ins141 |
| 5 | human | 29097 | CNIH4 | cornichon family AMPA recep... | NM_001277199.2 | 54.6% | 54.6% | 0_1ins189 |
| 6 | human | 29097 | CNIH4 | cornichon family AMPA recep... | NR_102347.2 | 9.1% | (many diffs) | |
| 7 | mouse | 98417 | Cnih4 | cornichon family AMPA recep... | NM_030131.3 | 89.6% | 94.9% | (many diffs) |
| 8 | mouse | 98417 | Cnih4 | cornichon family AMPA recep... | XM_011238890.2 | 73.6% | 59.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 483
- ORF length:
- 417
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ggcggtggag ttcgtcttct ctctcctcga ttgttgcgcg ctcatcttcc 121 tctcggTCTA CTTCATAATT ACATTGTCTG ATTTAGAATG TGATTACATT AATGCTAGAT 181 CATGTTGCTC AAAATTAAAC AAGTGGGTAA TTCCAGAATT GATTGGCCAT ACCATTGTCA 241 CTGTATTACT GCTCATGTCA TTGCACTGGT TCATCTTCCT TCTCAACTTA CCTGTTGCCA 301 CTTGGAATAT ATATCGATAC ATTATGGTGC CGAGTGGTAA CATGGGAGTG TTTGATCCAA 361 CAGAAATACA CAATCGAGGG CAGCTGAAGT CACACATGAA AGAAGCCATG ATCAAGCTTG 421 GTTTCCACTT GCTCTGCTTC TTCATGTATC TTTATAGTAT GATCTTAGCT TTGATAAATG 481 ACTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 541 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 601 GGCTTTATAT ATCTTGTGGA AAGGACGATC ACCACTGCGT TAATACACGG CAACGCGTTA 661 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt