Construct: ORF TRCN0000471080
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010669.1_s317c1
- Derived from:
- ccsbBroadEn_07032
- DNA Barcode:
- GGATTTCCTACCACAATTAACATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CNTN2 (6900)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471080
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6900 | CNTN2 | contactin 2 | NM_001346083.2 | 99.9% | 99.9% | 89G>A |
| 2 | human | 6900 | CNTN2 | contactin 2 | NM_005076.5 | 99.9% | 99.9% | 89G>A |
| 3 | human | 6900 | CNTN2 | contactin 2 | XM_024449389.1 | 97.9% | 96.5% | (many diffs) |
| 4 | human | 6900 | CNTN2 | contactin 2 | XM_017002198.1 | 87.5% | 83.3% | 89G>A;3014_3341del;3449_3561del |
| 5 | human | 6900 | CNTN2 | contactin 2 | XM_024449386.1 | 85.9% | 80.6% | (many diffs) |
| 6 | human | 6900 | CNTN2 | contactin 2 | XM_017002199.2 | 85.7% | 81.1% | (many diffs) |
| 7 | human | 6900 | CNTN2 | contactin 2 | XM_024449387.1 | 78% | 72.2% | (many diffs) |
| 8 | human | 6900 | CNTN2 | contactin 2 | XM_024449388.1 | 78% | 72.2% | (many diffs) |
| 9 | human | 6900 | CNTN2 | contactin 2 | NR_144350.2 | 38.6% | (many diffs) | |
| 10 | mouse | 21367 | Cntn2 | contactin 2 | NM_177129.5 | 85.8% | 90.2% | (many diffs) |
| 11 | mouse | 21367 | Cntn2 | contactin 2 | XM_006529362.2 | 85.8% | 90.2% | (many diffs) |
| 12 | mouse | 21367 | Cntn2 | contactin 2 | XR_878318.2 | 29.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3189
- ORF length:
- 3120
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggggacagcc accaggagga agccacacct gctgctggta gctgctgtgg 121 cccttgtctc ctcttcagct tggagttcag ccctggaatc ccaaaccacc ttcgggcctg 181 tctttgaaga ccagcccctc agtgtgctat tcccagagga gtccacggag gagcaggtgt 241 tgctggcatg ccgcgcccgg gccagccctc cagccaccta tcggtggaag atgaatggta 301 ccgagatgaa gctggagcca ggttcccgtc accagctggt ggggggcaac ctggtcatca 361 tgaaccccac caaggcacag gatgccgggg tctaccagtg cctggcctcc aacccagtgg 421 gcaccgttgt cagcagggag gccatcctcc gcttcggctt tctgcaggaa ttctccaagg 481 aggagcgaga cccagtgaaa gctcatgaag gctggggggt gatgttgccc tgtaacccac 541 ctgcccacta cccaggcttg tcctaccgct ggctcctcaa cgagttcccc aacttcatcc 601 cgacggacgg gcgtcacttc gtgtcccaga ccacagggaa cctgtacatt gcccgaacca 661 atgcctcaga cctgggcaac tactcctgtt tggccaccag ccacatggac ttctccacca 721 agagcgtctt cagcaagttt gctcagctca acctggctgc tgaagatacc cggctctttg 781 cacccagcat caaggcccgg ttcccagcag agacctatgc actggtgggg cagcaggtca 841 ccctggagtg cttcgccttt gggaaccctg tcccccggat caagtggcgc aaagtggacg 901 gctccctgtc cccgcagtgg accacagctg agcccaccct gcagatcccc agcgtcagct 961 ttgaggatga gggcacctac gagtgtgagg cggagaactc caagggccga gacaccgtgc 1021 agggccgcat catcgtgcag gctcagcctg agtggctaaa agtgatctcg gacacagagg 1081 ctgacattgg ctccaacctg cgttggggct gtgcagccgc cggcaagccc cggcctacag 1141 tgcgctggct gcggaacggg gagcctctgg cctcccagaa ccgggtggag gtgttggctg 1201 gggacctgcg gttctccaag ctgagcctgg aagactcggg catgtaccag tgtgtggcag 1261 agaataagca cggtaccatc tacgccagcg ccgagctagc cgtgcaagca ctcgcccctg 1321 acttcaggct gaatcccgtg aggcgtctga tccccgcggc ccgcggggga gagatcctta 1381 tcccctgcca gccccgggca gctccaaagg ccgtggtgct ctggagcaaa ggcacggaga 1441 ttttggtcaa cagcagcaga gtgactgtaa ctccagatgg caccttgatc ataagaaaca 1501 tcagccggtc agatgaaggc aaatacacct gctttgctga gaacttcatg ggcaaagcca 1561 acagcactgg aatcctatct gtgcgagatg caaccaaaat cactctagcc ccctcaagtg 1621 ccgacatcaa cttgggtgac aacctgaccc tacagtgcca tgcctcccac gaccccacca 1681 tggacctcac cttcacctgg accctggacg acttccccat cgactttgat aagcctggag 1741 ggcactaccg gagaactaat gtgaaggaga ccattgggga tctgaccatc ctgaacgccc 1801 agctgcgcca tggggggaag tacacgtgca tggcccagac ggtggtggac agcgcgtcca 1861 aggaggccac agtcctggtc cgaggtccgc caggtccccc aggaggtgtg gtggtgaggg 1921 acattggcga caccaccatc cagctcagct ggagccgtgg cttcgacaac cacagcccca 1981 tcgctaagta caccctgcaa gctcgcactc cacctgcagg gaagtggaag caggttcgga 2041 ccaatcctgc aaacatcgag ggcaatgccg agactgcaca ggtgctgggc ctcaccccct 2101 ggatggacta tgagttccgg gtcatagcca gcaacattct gggcactggg gagcctagtg 2161 ggccctccag caaaatccgg accagggaag cagccccctc ggtggcaccc tcaggactca 2221 gcggaggagg tggagccccc ggagagctca tcgtcaactg gacgcccatg tcacgggagt 2281 accagaacgg agacggcttc ggctacctgc tgtccttccg caggcagggc agcactcact 2341 ggcagaccgc ccgggtgcct ggcgccgatg cccagtactt tgtctacagc aacgagagcg 2401 tccggcccta cacgcccttt gaggtcaaga tccgcagcta caaccgccgc ggggatgggc 2461 ccgagagcct cactgcactc gtgtactcag ctgaggaaga gcccagggtg gcccctacca 2521 aggtgtgggc caaaggggtc tcatcctcag agatgaacgt gacctgggaa cccgtgcagc 2581 aggacatgaa tggtatcctc ctggggtatg agatccgcta ctggaaagct ggggacaaag 2641 aagcagctgc ggaccgagtg aggacagcag ggctggacac cagtgcccga gtcagcggcc 2701 tgcatcccaa caccaagtac catgtgaccg tgagggccta caaccgggct ggcactgggc 2761 ctgccagccc ttctgccaac gccacgacca tgaagccccc tccgcggcga cctcctggca 2821 acatctcctg gactttctca agctctagtc ttagcattaa gtgggaccct gtggtccctt 2881 tccgaaatga gtctgcagtc accggctata agatgctgta ccagaatgac ttacacctga 2941 ctcccacgct ccacctcacc ggcaagaact ggatagaaat cccagtgcct GAAGACATTG 3001 GCCATGCCCT GGTACAAATT CGGACCACAG GGCCCGGAGG GGATGGGATC CCTGCAGAAG 3061 TCCACATCGT GAGGAATGGA GGCACAAGCA TGATGGTGGA GAACATGGCA GTCCGCCCAG 3121 CACCACACCC TGGCACCGTC ATTTCCCACT CCGTGGCGAT GCTGATCCTC ATAGGCTCCC 3181 TGGAGCTCTT GCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 3241 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 3301 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAGGATTT CCTACCACAA TTAACATCAC 3361 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt