Construct: ORF TRCN0000471094

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF012718.1_s317c1
Derived from:
ccsbBroadEn_11046
DNA Barcode:
GGGAGACTGCATGAGGCTTGATTA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
POU2F1 (5451)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000471094
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5451 POU2F1 POU class 2 homeobox 1 XM_011509654.3 100% 100%
2 human 5451 POU2F1 POU class 2 homeobox 1 XM_024447727.1 100% 100%
3 human 5451 POU2F1 POU class 2 homeobox 1 NM_001198783.2 98.4% 98.4% 1_36del
4 human 5451 POU2F1 POU class 2 homeobox 1 NM_001365848.1 98% 95.6% (many diffs)
5 human 5451 POU2F1 POU class 2 homeobox 1 NM_001365849.1 98% 95.6% (many diffs)
6 human 5451 POU2F1 POU class 2 homeobox 1 NM_002697.4 96.9% 96.9% 1_69del
7 human 5451 POU2F1 POU class 2 homeobox 1 XM_011509653.2 96.6% 96.6% 1_78del
8 human 5451 POU2F1 POU class 2 homeobox 1 NM_001198786.2 88.7% 88.7% 1_69del;401_402ins189
9 human 5451 POU2F1 POU class 2 homeobox 1 XM_011509655.1 83.7% 83.4% (many diffs)
10 human 5451 POU2F1 POU class 2 homeobox 1 XM_017001508.2 46.1% 46% 1_78del;1136_1137insAG;1143_1144ins1162
11 human 5451 POU2F1 POU class 2 homeobox 1 NR_037163.2 15.9% 1_182del;2412_13944del
12 mouse 18986 Pou2f1 POU domain, class 2, transc... XM_017319360.1 90.1% 93.1% (many diffs)
13 mouse 18986 Pou2f1 POU domain, class 2, transc... NM_198933.3 88.7% 91.6% (many diffs)
14 mouse 18986 Pou2f1 POU domain, class 2, transc... NM_198932.3 87.5% 90.3% (many diffs)
15 mouse 18986 Pou2f1 POU domain, class 2, transc... XM_006496704.2 87.3% 90.2% (many diffs)
16 mouse 18986 Pou2f1 POU domain, class 2, transc... XM_011238767.2 87.3% 90.2% (many diffs)
17 mouse 18986 Pou2f1 POU domain, class 2, transc... XM_011238769.2 87.3% 90.2% (many diffs)
18 mouse 18986 Pou2f1 POU domain, class 2, transc... NM_198934.3 86% 88.8% (many diffs)
19 mouse 18986 Pou2f1 POU domain, class 2, transc... NM_011137.3 84.8% 87.6% (many diffs)
20 mouse 18986 Pou2f1 POU domain, class 2, transc... XR_373598.1 16.6% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2295
ORF length:
2229
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgaa caatccgtca gaaaccagta aaccatctat ggagagtgga gatggcaaca
121caggcacaca aaccaatggt ctggactttc agaagcagcc tgtgcctgta ggaggagcaa
181tctcaacagc ccaggcgcag gctttccttg gacatctcca tcaggtccaa ctcgctggaa
241caagtttaca ggctgctgct cagtctttaa atgtacagtc taaatctaat gaagaatcgg
301gggattcgca gcagccaagc cagccttccc agcagccttc agtgcaggca gccattcccc
361agacccagct tatgctagct ggaggacaga taactgggct tactttgacg cctgcccagc
421aacagttact actccagcag gcacaggcac aggcacagct gctggctgct gcagtgcagc
481agcactccgc cagccagcag cacagtgctg ctggagccac catctccgcc tctgctgcca
541cgcccatgac gcagatcccc ctgtctcagc ccatacagat cgcacaggat cttcaacaac
601tgcaacagct tcaacagcag aatctcaacc tgcaacagtt tgtgttggtg catccaacca
661ccaatttgca gccagcgcag tttatcatct cacagacgcc ccagggccag cagggtctcc
721tgcaagcgca aaatcttcta acgcaactac ctcagcaaag ccaagccaac ctcctacagt
781cgcagccaag catcaccctc acctcccagc cagcaacccc aacacgcaca atagcagcaa
841ccccaattca gacacttcca cagagccagt caacaccaaa gcgaattgat actcccagct
901tggaggagcc cagtgacctt gaggagcttg agcagtttgc caagaccttc aaacaaagac
961gaatcaaact tggattcact cagggtgatg ttgggctcgc tatggggaaa ctatatggaa
1021atgacttcag ccaaactacc atctctcgat ttgaagcctt gaacctcagc tttaagaaca
1081tgtgcaagtt gaagccactt ttagagaagt ggctaaatga tgcagagaac ctctcatctg
1141attcgtccct ctccagccca agtgccctga attctccagg aattgagggc ttgagccgta
1201ggaggaagaa acgcaccagc atagagacca acatccgtgt ggccttagag aagagtttct
1261tggagaatca aaagcctacc tcggaagaga tcactatgat tgctgatcag ctcaatatgg
1321aaaaagaggt gattcgtgtt tggttctgta accgccgcca gaaagaaaaa agaatcaacc
1381caccaagcag tggtgggacc agcagctcac ctattaaagc aattttcccc agcccaactt
1441cactggtggc gaccacacca agccttgtga ctagcagtgc agcaactacc ctcacagtca
1501gccctgtcct ccctctgacc agtgctgctg tgacgaatct ttcagttaca ggcacttcag
1561acaccacctc caacaacaca gcaaccgtga tttccacagc gcctccagct tcctcagcag
1621tcacgtcccc ctctctgagt ccctcccctt ctgcctcagc ctccacctcc gaggcatcca
1681gtgccagtga gaccagcaca acacagacca cctccactcc tttgtcctcc cctcttggga
1741ccagccaggt gatggtgaca gcatcaggtt tgcaaacagc agcagctgct gcccttcaag
1801gagctgcaca gttgccagca aatgccagtc ttgctgccat ggcagctgct gcaggactaa
1861acccaagccT GATGGCACCC TCACAGTTTG CGGCTGGAGG TGCCTTACTC AGTCTGAATC
1921CAGGGACCCT GAGCGGTGCT CTCAGCCCAG CTCTAATGAG CAACAGTACA CTGGCAACTA
1981TTCAAGCTCT TGCTTCTGGT GGCTCTCTTC CAATAACATC ACTTGATGCA ACTGGGAACC
2041TGGTATTTGC CAATGCGGGA GGAGCCCCCA ACATCGTGAC TGCCCCTCTG TTCCTGAACC
2101CTCAGAACCT CTCTCTGCTC ACCAGCAACC CTGTTAGCTT GGTCTCTGCC GCCGCAGCAT
2161CTGCAGGGAA CTCTGCACCT GTAGCCAGCC TTCACGCCAC CTCCACCTCT GCTGAGTCCA
2221TCCAGAACTC TCTCTTCACA GTGGCCTCTG CCAGCGGGGC TGCGTCCACC ACCACCACCG
2281CCTCCAAGGC ACAGTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC
2341CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA
2401TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA GGGAGACTGC ATGAGGCTTG
2461ATTAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)