Construct: ORF TRCN0000471139

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014527.2_s317c1
Derived from:
ccsbBroadEn_10928
DNA Barcode:
GACACCTTCTTAACTGTTGTGGTC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ITPKB (3707)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000471139
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 3707 ITPKB inositol-trisphosphate 3-ki... XM_005273120.3 99.8% 99.5% 518G>A;1222T>G;1655C>A
2 human 3707 ITPKB inositol-trisphosphate 3-ki... XM_017001211.2 88% 87.8% (many diffs)
3 human 3707 ITPKB inositol-trisphosphate 3-ki... NM_002221.3 67.9% 67.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1998
ORF length:
1932
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc tgtgtactgc tatgcgctca atagcctggt gatcatgaat agcgccaacg
121agatgaagag cggcggcggc ccggggccca gtggcagcga gacgcccccg cccccgagga
181gggcagtgct gagccccggc agcgttttca gccccgggag aggcgcctct ttcctcttcc
241ccccagccga gtcgctgtcc cccgaggagc cccggagccc cgggggctgg cggagcggcc
301ggcgcaggct gaatagtagc agcggcagtg gcagcggcag cagcggcagt agcgtgagca
361gcccaagttg ggctggtcgc ctgcgagggg accggcagca ggtggtggca gccggtaccc
421tctccccgcc agggccggag gaggccaaga ggaagctgcg gatcttgcag cgcgagttgc
481agaacgtgca ggtgaaccag aaagtgggca tgtttgaggc gcacatccag gcacagagct
541ccgccattca agcgccccgc agcccgcgtt tgggcagggc tcactcgccc tccccgtgcc
601ccttccgcag cagcagtcag ccccctggaa gggtcctggt tcagggcgcc cggagcgagg
661aacggaggac aaagtcctgg ggggagcaat gtccagagac ttcaggaacc gactccggga
721ggaaaggagg gcccagccta tgctcctcgc aggtgaagaa aggaatgcca cctcttcccg
781gccgggctgc ccctacagga tcagaggctc agggtccatc cgcttttgta aggatggaga
841agggtatccc tgccagtccc cgctgtggct cacccacagc tatggaaatt gacaaaaggg
901gctctcctac cccgggaact cggagctgcc tagctccctc attggggctg ttcggagcta
961gcttaacgat ggccacggaa gtggcagcga gagttacatc cactgggcca caccgtccac
1021aggatcttgc cctcactgag ccgtctggga gagcccgtga gcttgaggac ctgcagcccc
1081cagaggccct ggtggagagg caggggcagt ttctgggcag tgagacaagc ccagccccag
1141aaaggggcgg gccccgcgat ggagaacccc ctgggaagat ggggaaagga tatctgccct
1201gtggcatgcc gggctctggg gagcctgaag tgggcaaaag gccagaggag acgactgtga
1261gcgtgcaaag cgcagagtcc tctgatgccc tgagctggtc caggctgccc agggccctgg
1321cctccgtagg ccctgaggag gcccgaagtg gggcccccgt gggcgggggg cgttggcagc
1381tctccgacag agtggaggga gggtccccaa cgctgggctt gcttgggggc agcccctcag
1441cacagccggg gaccgggaat gtggaggcgg gaattccttc tggcagaatg ctggagcctt
1501tgccctgttg ggacgctgcg aaagatctga aagaacctca gtgccctcct ggggacaggg
1561tgggtgtgca gccTGGGAAC TCCAGGGTTT GGCAGGGCAC CATGGAGAAA GCCGGTTTGG
1621CTTGGACGCG TGGCACAGGG GTGCAATCAG AGGGGACTTG GGAAAGCCAG CGGCAGGACA
1681GTGATGCCCT CCCAAGTCCG GAGCTGCTAC CCCAAGATCA GGACAAGCCT TTCCTGAGGA
1741AGGCCTGCAG CCCCAGCAAC ATACCTGCTG TCATCATTAC AGACATGGGC ACCCAGGAGG
1801ATGGGGCCTT GGAGGAGACG CAGGGAAGCC CTCGGGGCAA CCTGCCCCTG AGGAAACTGT
1861CCTCTTCCTC GGCCTCCTCC ACGGGCTTCT CCTCATCCTA CGAAGACTCA GAGGAGGACA
1921TCTCCAGTGA CCCTGAGCGC ACCCTGGACC CCAACTCAGC CTTCCTGCAT ACCCTGGACC
1981AGCAGAAACC TAGAGTGTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA
2041TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA
2101GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAGACACCT TCTTAACTGT
2161TGTGGTCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)