Construct: ORF TRCN0000471142
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012879.1_s317c1
- Derived from:
- ccsbBroadEn_10898
- DNA Barcode:
- CTAAGACAGGCGATTACCCGGCTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IDI1 (3422)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471142
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3422 | IDI1 | isopentenyl-diphosphate del... | NM_001317955.2 | 99.8% | 99.5% | 304G>A |
| 2 | human | 3422 | IDI1 | isopentenyl-diphosphate del... | NM_001317956.2 | 99.8% | 99.5% | 304G>A |
| 3 | human | 3422 | IDI1 | isopentenyl-diphosphate del... | XM_024447979.1 | 81.1% | 78.6% | (many diffs) |
| 4 | human | 3422 | IDI1 | isopentenyl-diphosphate del... | XM_017016191.2 | 80.6% | 76% | (many diffs) |
| 5 | human | 3422 | IDI1 | isopentenyl-diphosphate del... | NM_001317957.2 | 80.2% | 79.8% | (many diffs) |
| 6 | human | 3422 | IDI1 | isopentenyl-diphosphate del... | NM_004508.3 | 80.1% | 79.9% | 1_168del;472G>A |
| 7 | human | 3422 | IDI1 | isopentenyl-diphosphate del... | XM_024447980.1 | 79.5% | 78% | (many diffs) |
| 8 | human | 3422 | IDI1 | isopentenyl-diphosphate del... | NR_134301.1 | 22.1% | (many diffs) | |
| 9 | human | 3422 | IDI1 | isopentenyl-diphosphate del... | NR_134300.2 | 20.7% | (many diffs) | |
| 10 | mouse | 102635781 | Gm38481 | predicted gene, 38481 | XM_006498513.3 | 84.5% | 85.9% | (many diffs) |
| 11 | mouse | 319554 | Idi1 | isopentenyl-diphosphate del... | NM_145360.2 | 68.1% | 69.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 750
- ORF length:
- 684
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat gcctgaaata aacactaacc acctcgacaa gcaacaggtt caactcctgg 121 cagagatgtg tatccttatt gatgaaaatg acaataaaat tggagctgag accaagaaga 181 attgtcacct gaacgagaac attgagaaag gattattgca tcgagctttt agtgtcttct 241 tattcaacac cgaaaataag cttctgctac agcaaagatc agatgctaag attacctttc 301 caggttgttt tacgaatacg tgttGTAGTC ATCCATTAAG CAATCCAGCC GAGCTTGAGG 361 AAAGTGACAC CCTTGGAGTG AGGCGAGCAG CACAGAGACG GCTGAAAGCT GAGCTAGGAA 421 TTCCCTTGGA AGAGGTTCCT CCAGAAGAAA TTAATTATTT AACACGAATT CACTACAAAG 481 CTCAGTCTGA TGGTATCTGG GGTGAACATG AAATTGATTA CATTTTGTTG GTGAGGAAGA 541 ATGTAACTTT GAATCCAGAT CCCAATGAGA TTAAAAGCTA TTGTTATGTG TCAAAGGAAG 601 AACTAAAAGA ACTTCTGAAA AAAGCAGCCA GTGGTGAAAT TAAGATAACG CCATGGTTTA 661 AAATTATTGC AGCGACTTTT CTCTTTAAAT GGTGGGATAA CTTAAATCAT TTGAATCAGT 721 TTGTTGACCA TGAGAAAATA TACAGAATGT GCCCAACTTT CTTGTACAAA GTGGTTGATA 781 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 841 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACTAAG 901 ACAGGCGATT ACCCGGCTAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 961 tgaaagatt