Transcript: Human XM_017016191.2

PREDICTED: Homo sapiens isopentenyl-diphosphate delta isomerase 1 (IDI1), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
IDI1 (3422)
Length:
3852
CDS:
2041..2688

Additional Resources:

NCBI RefSeq record:
XM_017016191.2
NBCI Gene record:
IDI1 (3422)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017016191.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000049173 CGTGTTGTAGTCATCCATTAA pLKO.1 2255 CDS 100% 13.200 9.240 N IDI1 n/a
2 TRCN0000290654 CGTGTTGTAGTCATCCATTAA pLKO_005 2255 CDS 100% 13.200 9.240 N IDI1 n/a
3 TRCN0000049174 GCCAGTGGTGAAATTAAGATA pLKO.1 2563 CDS 100% 5.625 3.938 N IDI1 n/a
4 TRCN0000290723 GCCAGTGGTGAAATTAAGATA pLKO_005 2563 CDS 100% 5.625 3.938 N IDI1 n/a
5 TRCN0000049177 TGGTGGGATAACTTAAATCAT pLKO.1 2626 CDS 100% 5.625 3.938 N IDI1 n/a
6 TRCN0000290653 TGGTGGGATAACTTAAATCAT pLKO_005 2626 CDS 100% 5.625 3.938 N IDI1 n/a
7 TRCN0000049176 ACAGCAAAGATCAGATGCTAA pLKO.1 2205 CDS 100% 4.950 3.465 N IDI1 n/a
8 TRCN0000049175 CCAGATCCCAATGAGATTAAA pLKO.1 2491 CDS 100% 15.000 9.000 N IDI1 n/a
9 TRCN0000290725 CCAGATCCCAATGAGATTAAA pLKO_005 2491 CDS 100% 15.000 9.000 N IDI1 n/a
10 TRCN0000074123 CGCCTGTAATCCCAACACTTT pLKO.1 227 5UTR 100% 4.950 2.475 Y GJD4 n/a
11 TRCN0000166650 CGCCTGTAATCCCAACACTTT pLKO.1 227 5UTR 100% 4.950 2.475 Y C9orf85 n/a
12 TRCN0000179120 CAACATGGTGAAACCCTGTTT pLKO.1 300 5UTR 100% 4.950 2.475 Y LOC339059 n/a
13 TRCN0000138772 GCAGGAGAATCGCTTGAACTT pLKO.1 392 5UTR 100% 4.950 2.475 Y DCAF11 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017016191.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_10897 pDONR223 100% 80.8% 76.4% None (many diffs) n/a
2 TRCN0000473963 TCAAGCGGATCTCGCTTAGTCTGT pLX_317 74.7% 80.8% 76.4% V5 (many diffs) n/a
3 ccsbBroadEn_10898 pDONR223 100% 80.6% 76% None (many diffs) n/a
4 ccsbBroad304_10898 pLX_304 0% 80.6% 76% V5 (many diffs) n/a
5 TRCN0000471142 CTAAGACAGGCGATTACCCGGCTA pLX_317 61.9% 80.6% 76% V5 (many diffs) n/a
6 ccsbBroadEn_15460 pDONR223 0% 80.6% 76% None (many diffs) n/a
7 ccsbBroad304_15460 pLX_304 0% 80.6% 76% V5 (many diffs) n/a
8 TRCN0000479160 TTTGCTGATTAGCCTGACGATGTA pLX_317 59% 80.6% 76% V5 (many diffs) n/a
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