Construct: ORF TRCN0000471165
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005849.1_s317c1
- Derived from:
- ccsbBroadEn_09782
- DNA Barcode:
- TCGTGAAGCGCCATCCACTGCGAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NUDT10 (170685)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471165
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 170685 | NUDT10 | nudix hydrolase 10 | NM_001304963.2 | 99.3% | 99.3% | 201C>T;310C>T;403G>A |
| 2 | human | 170685 | NUDT10 | nudix hydrolase 10 | NM_153183.3 | 99.3% | 99.3% | 201C>T;310C>T;403G>A |
| 3 | human | 55190 | NUDT11 | nudix hydrolase 11 | NM_018159.4 | 98.3% | 98.7% | (many diffs) |
| 4 | human | 440672 | NUDT4B | nudix hydrolase 4B | NM_001355407.1 | 73.1% | 81.2% | (many diffs) |
| 5 | human | 11163 | NUDT4 | nudix hydrolase 4 | NM_199040.4 | 73.1% | 81.2% | (many diffs) |
| 6 | human | 11163 | NUDT4 | nudix hydrolase 4 | NM_019094.6 | 72.7% | 80.6% | (many diffs) |
| 7 | mouse | 58242 | Nudt11 | nudix (nucleoside diphospha... | NM_021431.2 | 87.6% | 94.5% | (many diffs) |
| 8 | mouse | 102954 | Nudt10 | nudix (nucleoside diphospha... | NM_001031664.1 | 87.3% | 94.5% | (many diffs) |
| 9 | mouse | 102954 | Nudt10 | nudix (nucleoside diphospha... | XM_006527546.2 | 87.3% | 94.5% | (many diffs) |
| 10 | mouse | 71207 | Nudt4 | nudix (nucleoside diphospha... | XM_006514116.2 | 74.3% | 82.2% | (many diffs) |
| 11 | mouse | 71207 | Nudt4 | nudix (nucleoside diphospha... | NM_027722.4 | 73.9% | 81.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 558
- ORF length:
- 492
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gtgcaaaccc aaccagacac ggaccTACGA CCCCGAGGGG TTCAAGAAGC 121 GGGCGGCGTG CCTGTGCTTC CGGAGCGAGC GCGAGGACGA GGTCCTGTTA GTGAGTAGCA 181 GCCGGTACCC GGACCGCTGG ATCGTGCCGG GCGGGGGCAT GGAGCCCGAG GAGGAGCCGG 241 GCGGTGCGGC GGTCCGAGAG GTGTATGAAG AGGCGGGAGT CAAGGGGAAG TTAGGCCGGC 301 TCCTGGGCGT CTTCGAACAG AACCAGGACC CCAAGCACAG AACGTACGTG TATGTACTGA 361 CTGTCACGGA GCTGTTGGAG GATTGGGAAG ATTCGGTTAG CATTGGGAGG AAGCGAGAGT 421 GGTTCAAAGT CGAAGATGCC ATCAAGGTTC TCCAGTGCCA CAAGCCCATG CACGCCGAAT 481 ATCTGGAGAA ACTAAAGCTG GGCGGTTCCC CAACCAATGG AAACTCCATG GCCCCATCCT 541 CGCCAGATAG CGATCCCTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 601 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 661 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATCGTGAA GCGCCATCCA 721 CTGCGAAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt