Construct: ORF TRCN0000471183
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016850.1_s317c1
- Derived from:
- ccsbBroadEn_02352
- DNA Barcode:
- GCCTTTTACTTTGTGCATCCCTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NUTF2 (10204)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471183
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10204 | NUTF2 | nuclear transport factor 2 | NM_001322038.2 | 100% | 100% | |
| 2 | human | 10204 | NUTF2 | nuclear transport factor 2 | NM_001322039.2 | 100% | 100% | |
| 3 | human | 10204 | NUTF2 | nuclear transport factor 2 | NM_001322040.2 | 100% | 100% | |
| 4 | human | 10204 | NUTF2 | nuclear transport factor 2 | NM_001322041.2 | 100% | 100% | |
| 5 | human | 10204 | NUTF2 | nuclear transport factor 2 | NM_005796.3 | 100% | 100% | |
| 6 | mouse | 68051 | Nutf2 | nuclear transport factor 2 | NM_026532.3 | 94.2% | 100% | (many diffs) |
| 7 | mouse | 68051 | Nutf2 | nuclear transport factor 2 | XM_006531325.3 | 94.2% | 100% | (many diffs) |
| 8 | mouse | 621832 | Nutf2-ps2 | nuclear transport factor 2,... | XM_001473957.5 | 93.9% | 100% | (many diffs) |
| 9 | mouse | 100043462 | Nutf2-ps1 | nuclear transport factor 2,... | NR_033574.1 | 39.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 447
- ORF length:
- 381
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttGGCATGGG AGACAAGCCA ATTTGGGAGC AGATTGGATC CAGCTTCATT CAACATTACT 121 ACCAGTTATT TGATAATGAT AGAACCCAAC TAGGCGCAAT TTACATTGAC GCGTCATGCC 181 TTACGTGGGA AGGACAACAG TTCCAGGGGA AAGCTGCCAT TGTGGAGAAG TTGTCTAGCC 241 TTCCGTTCCA GAAAATTCAG CACAGCATCA CCGCGCAGGA CCATCAGCCC ACTCCAGATA 301 GCTGCATCAT CAGCATGGTT GTGGGCCAGC TTAAGGCGGA TGAAGACCCC ATCATGGGGT 361 TCCACCAGAT GTTCCTATTA AAGAACATCA ACGATGCTTG GGTTTGCACC AATGACATGT 421 TCAGGCTCGC CCTGCACAAC TTTGGCTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 481 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 541 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGCCTTTTA 601 CTTTGTGCAT CCCTACACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 661 aagatt