Construct: ORF TRCN0000471201
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003551.3_s317c1
- Derived from:
- ccsbBroadEn_08577
- DNA Barcode:
- TCAACACGTGCAGTGTTACCCGGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AGK (55750)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471201
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55750 | AGK | acylglycerol kinase | NM_018238.4 | 99.9% | 100% | 228C>T |
| 2 | human | 55750 | AGK | acylglycerol kinase | XM_011516397.3 | 99.9% | 100% | 228C>T |
| 3 | human | 55750 | AGK | acylglycerol kinase | XM_024446835.1 | 99.9% | 100% | 228C>T |
| 4 | human | 55750 | AGK | acylglycerol kinase | NM_001364948.2 | 82.7% | 82.7% | 228C>T;1045delG;1050_1051ins217 |
| 5 | mouse | 69923 | Agk | acylglycerol kinase | NM_023538.2 | 87.5% | 91.7% | (many diffs) |
| 6 | mouse | 69923 | Agk | acylglycerol kinase | XM_011241458.1 | 83.6% | 85.7% | (many diffs) |
| 7 | mouse | 69923 | Agk | acylglycerol kinase | XM_006506571.2 | 67.9% | 70.1% | (many diffs) |
| 8 | mouse | 69923 | Agk | acylglycerol kinase | XM_017321733.1 | 43.5% | 44.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1332
- ORF length:
- 1266
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac ggtgttcttt aaaacgcttc gaaatcactg gaagaaaact acagctgggc 121 tctgcctgct gacctgggga ggccattggc tctatggaaa acactgtgat aacctcctaa 181 ggagagcagc ctgtcaagaa gctcaggtgt ttggcaatca actcattcct cccaatgcac 241 aagtgaagaa ggccactgtt tttctcaatc ctgcagcttg caaaggaaaa gctaggactc 301 tatttgaaaa aaatgctgcc ccgattttac atttatctgg catggatgtg actattgtta 361 agacagatta tgagggacaa gccaagaaac tcctggaact gatggaaaac acggatgtga 421 tcattgttgc aggaggagat gggacactgc aggaggttgt tactggtgtt cttcgacgaa 481 cagatgaggc taccttcagt aagattccca ttggatttat cccactggga gagaccagta 541 gtttgagtca taccctcttt gccgaaagtg gaaacaaagt ccaacatatt actgatgcca 601 cacttgccat tgtgaaagga gagacagttc cacttgatgt cttgcagatc aagggtgaaa 661 aggaacagcc tgtatttgca atgaccggcc ttcgatgggg atctttcaga gatgctggcg 721 tcaaagttag caagtactgg tatcttgggc ctctaaaaat caaagcagcc cactttttca 781 gcactcttaa ggagtggcct cagactcatc aagcctctat ctcatacacg ggacctacag 841 agagacctcc caatgaacca gaggagaccc ctgtacaaag gccttctttg tacaggagaa 901 tattacgaag gcttgcgTCC TACTGGGCAC AACCACAGGA TGCCCTTTCC CAAGAGGTGA 961 GCCCGGAGGT CTGGAAAGAT GTGCAGCTGT CCACCATTGA ACTGTCCATC ACAACACGGA 1021 ATAATCAGCT TGACCCGACA AGCAAAGAAG ATTTTCTGAA TATCTGCATT GAACCTGACA 1081 CCATCAGCAA AGGAGACTTT ATAACTATAG GAAGTCGAAA GGTGAGAAAC CCCAAGCTGC 1141 ACGTGGAGGG CACGGAGTGT CTCCAAGCCA GCCAGTGCAC TTTGCTTATC CCGGAGGGAG 1201 CAGGGGGCTC TTTTAGCATT GACAGTGAGG AGTATGAAGC GATGCCTGTG GAGGTGAAAC 1261 TGCTCCCCAG GAAGCTGCAG TTCTTCTGTG ATCCTAGGAA GAGAGAACAG ATGCTCACAA 1321 GCCCCACCCA GTGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1381 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1441 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATCA ACACGTGCAG TGTTACCCGG 1501 GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t